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The Rangeland Journal The Rangeland Journal Society
Journal of the Australian Rangeland Society
RESEARCH ARTICLE

Sampling requirements for predicting cattle diet quality using faecal near-infrared reflectance spectroscopy (F.NIRS) in heterogeneous tropical rangeland pastures

I. A. White A B D , L. P. Hunt B , D. P. Poppi A and S. R. Petty C
+ Author Affiliations
- Author Affiliations

A Schools of Animal Studies and Veterinary Science, University of Queensland, Gatton, Qld 4343, Australia.

B CSIRO Ecosystem Sciences, PMB 44, Winnellie, NT 0822, Australia.

C Northern Development Company, PO Box 447, Kununurra, WA 6743, Australia.

D Corresponding author. Email: G-sec@hotmail.com

The Rangeland Journal 32(4) 435-441 https://doi.org/10.1071/RJ09021
Submitted: 26 March 2009  Accepted: 26 October 2010   Published: 26 November 2010

Abstract

Faecal near-infrared reflectance spectroscopy (F.NIRS) provides predictive information on cattle diets and nutritional levels, useful for livestock management or for research purposes. Potential errors exist throughout the entire F.NIRS process, including the collection method. The accepted collection method involves aggregating equal amounts of faecal material from 5 to 15 animals, mixing and removing a single sample for analysis. The adequacy of this method was tested by collecting and analysing up to 70 samples from individual cattle in different paddocks. Two methods were used to determine sample size based on observed variability in dietary attributes. Variability of dietary non-grass material and crude protein content increased with paddock size, so required sample size also increased. For dietary F.NIRS predictions to be used for research, our results suggest from 20 to 51 samples are needed in small to large paddocks to accurately predict the proportion of dietary non-grass material, from 12 to 50 samples for crude protein content and from 6 to 34 samples for dry matter digestibility. Composite samples from 15 cattle provided representative means in less than 50% of the situations investigated using biologically significant precision levels, but would be adequate for management of animal nutrition. Analysis of individual samples provided additional measures of range and variability which were also informative.


References

Barnes, M. K., Norton, B. E., Maeno, M., and Malechek, J. C. (2008). Paddock size and stocking density affect spatial heterogeneity of grazing. Rangeland Ecology and Management 61, 380–388.
Paddock size and stocking density affect spatial heterogeneity of grazing.Crossref | GoogleScholarGoogle Scholar |

Coates, D. B. (1999). Faecal spectroscopy (NIRS) for nutritional profiling of grazing cattle. In: ‘People and rangelands, building the future. Proceedings of the VIth International Rangeland Congress’. (Eds D. Eldridge and D. Freudenberger.) pp. 466–467. (VIth International Rangeland Congress Inc.: Aitkenvale, Qld.)

Coates, D. B. (2000). Faecal NIRS – what does it offer today’s grazier? Tropical Grasslands 34, 230–239.

Coates, D. B. (2004). Faecal NIRS – technology for improving nutritional management of grazing cattle. Final Report of Project NAP3.121, Meat and Livestock Australia, Sydney.

Coates, D. B., and Dixon, R. M. (2007). Faecal near infrared reflectance spectroscopy (F.NIRS) measurement of non-grass proportions in the diet of cattle grazing tropical rangelands. The Rangeland Journal 29, 51–63.
Faecal near infrared reflectance spectroscopy (F.NIRS) measurement of non-grass proportions in the diet of cattle grazing tropical rangelands.Crossref | GoogleScholarGoogle Scholar |

Coates, D. B., Van der Weide, A. P. A., and Kerr, J. D. (1991). Changes in faecal δ13C in response to changing proportions of legume (C3) and grass (C4) in the diet of sheep and cattle. Journal of Agricultural Science, Cambridge 116, 287–295.
Changes in faecal δ13C in response to changing proportions of legume (C3) and grass (C4) in the diet of sheep and cattle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXlsFOrt7o%3D&md5=c299770252708eaf5b0958774acaabe1CAS |

Dixon, R., and Coates, D. (2009). Near infrared spectroscopy of faeces to evaluate the nutrition and physiology of herbivores. Journal of Near Infrared Spectroscopy 17, 1–31.
Near infrared spectroscopy of faeces to evaluate the nutrition and physiology of herbivores.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXis1ahtLk%3D&md5=9ff06ee57728474d8286b5a2f7144e56CAS |

Dove, H., and Mayes, R. W. (2005). Using n-alkanes and other plant wax components to estimate intake, digestibility and diet composition of grazing/browsing sheep and goats. Small Ruminant Research 59, 123–139.
Using n-alkanes and other plant wax components to estimate intake, digestibility and diet composition of grazing/browsing sheep and goats.Crossref | GoogleScholarGoogle Scholar |

Dove, H., and Mayes, R. W. (2006). Protocol for the analysis of n-alkanes and other plant-wax compounds and for their use as markers for quantifying the nutrient supply of large mammalian herbivores. Nature Protocols 1, 1680–1697.
Protocol for the analysis of n-alkanes and other plant-wax compounds and for their use as markers for quantifying the nutrient supply of large mammalian herbivores.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtFagtrfE&md5=41dd95bc6f7f84ce4fb39a2fe7187f16CAS | 17487151PubMed |

Gardener, C. J., McIvor, J. G., and Jansen, A. (1993). Passage of legume and grass seeds through the digestive tract of cattle and their survival in faeces. Journal of Applied Ecology 30, 63–74.
Passage of legume and grass seeds through the digestive tract of cattle and their survival in faeces.Crossref | GoogleScholarGoogle Scholar |

Hodgkinson, K. C., Terpstra, J. W., and Muller, J. W. (1995). Spatial and temporal pattern in the grazing of grasses by sheep within a semi-arid wooded landscape. The Rangeland Journal 17, 154–165.
Spatial and temporal pattern in the grazing of grasses by sheep within a semi-arid wooded landscape.Crossref | GoogleScholarGoogle Scholar |

iSixSigma Staff (2000). How to determine sample size, determining sample size. Available at: www.isixsigma.com/library/content/c000709a.asp (accessed 11 October 2008).

Jones, R. J., and Jones, R. M. (1997). Grazing management in the tropics. In: ‘Proceedings of the XVIII International Grassland Congress’. Winnipeg, MB and Saskatoon, SK. (Ed. J. G. Buchanan-Smith.) pp. 535–542. (Grasslands 2000, Winnipeg, MB, Canada.) Available at: www.internationalgrasslands.org/publications/pdfs/1997/III_535.PDF

Laca, E. A. (2009). New approaches and tools for grazing management. Rangeland Ecology and Management 62, 407–417.
New approaches and tools for grazing management.Crossref | GoogleScholarGoogle Scholar |

Lyons, R. K., and Stuth, J. W. (1992). Fecal NIRS equations for predicting diet quality of free-ranging cattle. Journal of Range Management 45, 238–244.
Fecal NIRS equations for predicting diet quality of free-ranging cattle.Crossref | GoogleScholarGoogle Scholar |

McCafferty, P., Krebs, G., Ho, K., Dods, K., and Kumara Mahipala, P. (2008). Utilisation of near infrared spectroscopy to optimise productivity of grazing animal in the Western Australian rangelands. In: ‘Multifunctional Grasslands in a Changing World’. Vol. 2. (Eds Organizing Committee of IGC/IRC Congress.) p. 58. (Guangdong People’s Publishing House: Guangdong, China.)

Millard, S. P. (2001). ‘Environmental Stats for S-PLUS Help. Version 2.0.’ (Probability, Statistics and Information: Seattle, WA.)

Millard, S. P., and Neerchal, N. K. (2001). ‘Environmental Statistics.’ (CRC Press: Boca Raton, FL.)

Petty, S. R., Hunt, L. P., Cowley, R. A., McCosker, K. D., and Fisher, A. (2007). Recent advances in grazing management in northern Australia – the Pigeon Hole experience. Recent Advances in Animal Nutrition in Australia 16, 235–239.

Reich, M. M., Hendricksen, R. E., Rideout, J. A., Gazzola, C., and Hill, R. A. (2000). Alkane profiles in tropical forages. Asian-Australasian Journal of Animal Sciences 13, 152.

Sapienza, D., Berzaghi, P., Martin, N., Taysom, D., Owens, F., Mahanna, B., Sevenich, D., and Allen, R. (2008). NIRS White Paper. Near Infrared Spectroscopy for forage and feed testing. Available at: www.uwex.edu/ces/forage/NIRS/nirs_white_paper.pdf (accessed 28 April 2009).

Stokes, C. J., McAllister, R. R. J., and Ash, A. J. (2006). Fragmentation of Australian rangelands: processes, benefits and risks of changing patterns of land use. The Rangeland Journal 28, 83–96.
Fragmentation of Australian rangelands: processes, benefits and risks of changing patterns of land use.Crossref | GoogleScholarGoogle Scholar |

Taylor, J. A., and Cook, S. J. (1993). Management and use of complex landscapes: some implications of spatial variation for pasture and animal production. In: ‘Proceedings of the XVII International Grassland Congress’. New Zealand. pp. 1932–1934. (New Zealand Grassland Association: Palmerston North, NZ)

Taylor, J. A., and Tulloch, D. (1985). Rainfall in the wet-dry tropics: extreme events at Darwin and similarities between years during the period 1870–1983 inclusive. Australian Journal of Ecology 10, 281–295.
Rainfall in the wet-dry tropics: extreme events at Darwin and similarities between years during the period 1870–1983 inclusive.Crossref | GoogleScholarGoogle Scholar |

Teague, W. R., and Dowhower, S. L. (2003). Patch dynamics under rotational and continuous grazing management in large, heterogeneous paddocks. Journal of Arid Environments 53, 211–229.
Patch dynamics under rotational and continuous grazing management in large, heterogeneous paddocks.Crossref | GoogleScholarGoogle Scholar |

Tothill, J. C., Hargreaves, J. N. G., Jones, R. M., and MacDonald, C. K. (1992). BOTANAL – a comprehensive sampling and computing procedure for estimating pasture yield and composition. 1. Field sampling. Tropical Agronomy Technical Memorandum No. 78. CSIRO Division of Tropical Crops and Pastures, Brisbane.