Non-invasive assessment of oocyte developmental competence
Tiffany C. Y. Tan
A B C and Kylie R. Dunning A B C *
+ Author Affiliations
- Author Affiliations
A Robinson Research Institute, School of Biomedicine, The University of Adelaide, Adelaide, SA, Australia.
B Australian Research Council Centre of Excellence for Nanoscale Biophotonics, The University of Adelaide, Adelaide, SA, Australia.
C Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, SA, Australia.
* Correspondence to: kylie.dunning@adelaide.edu.au
Reproduction, Fertility and Development 35(2) 39-50 https://doi.org/10.1071/RD22217
Published online: 18 October 2022
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing on behalf of the IETS. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)
Abstract
Oocyte quality is a key factor influencing IVF success. The oocyte and surrounding cumulus cells, known collectively as the cumulus oocyte complex (COC), communicate bi-directionally and regulate each other’s metabolic function to support oocyte growth and maturation. Many studies have attempted to associate metabolic markers with oocyte quality, including metabolites in follicular fluid or ‘spent medium’ following maturation, gene expression of cumulus cells and measuring oxygen consumption in medium surrounding COCs. However, these methods fail to provide spatial metabolic information on the separate oocyte and cumulus cell compartments. Optical imaging of the autofluorescent cofactors – reduced nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] and flavin adenine dinucleotide (FAD) – has been put forward as an approach to generate spatially resolved measurements of metabolism within individual cells of the COC. The optical redox ratio (FAD/[NAD(P)H + FAD]), calculated from these cofactors, can act as an indicator of overall metabolic activity in the oocyte and cumulus cell compartments. Confocal microscopy, fluorescence lifetime imaging microscopy (FLIM) and hyperspectral microscopy may be used for this purpose. This review provides an overview of current optical imaging techniques that capture the inner biochemistry within cells of the COC and discusses the potential for such imaging to assess oocyte developmental competence.
Keywords: autofluorescence, cellular metabolism, FAD, NAD(P)H, non-invasive, oocyte assessment, optical imaging, optical redox ratio.
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