Glucose consumption and gene expression in granulosa cells collected before and after in vitro oocyte maturation in the southern white rhinoceros (Ceratotherium simum simum)
E. Ruggeri
A * , C. Young A , N. Ravida A , M. A. Sirard B , R. Krisher C , M. de la Rey D , C. Herbst D and B. Durrant A
+ Author Affiliations
- Author Affiliations
A Reproductive Sciences, Beckman Center for Conservation Research, San Diego Zoo Wildlife Alliance, 15600 San Pasqual Valley Road, Escondido, CA 92027, USA.
B Departement des Sciences Animales, Centre de Recherce en Reproduction, Développement et Santé Inter-générationnelle (CRDSI), Université Laval, Pavillion Des Services, Local 2732, Quebec, QC G1V 0A6, Canada.
C Genus PLC, 1525 River Road, De Forest, WI 53532, USA.
D Embryo Plus, 41 Hendrik Vrewoerd Avenue, Brits 0250, South Africa.
Reproduction, Fertility and Development 34(13) 875-888 https://doi.org/10.1071/RD22071
Published online: 25 July 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)
Abstract
Context: With two northern white rhinos (NWR) remaining, the continued existence of this species relies on studying their relative, the southern white rhino (SWR).
Aims: (1) Characterise gene expression in granulosa cells (GC) from SWR cumulus oocyte complexes (COCs) prior to (Pre-) and after (Post-) in vitro maturation (IVM), comparing culture media and oocytes from donors treated with or without gonadotropin stimulation prior to ovum recovery; and (2) evaluate COC glucose consumption in spent media.
Methods: COCs were retrieved from four SWRs. Granulosa cells were collected before and after IVM in SDZ or IZW medium. Total RNA was evaluated by qPCR.
Key results: Oocyte maturation was greater in SDZ than IZW media. Expression of genes associated with follicle development increased in Pre-IVM GC. Six genes were differentially expressed in Post-IVM GC from stimulated compared to unstimulated donors. COCs from stimulated animals consumed more glucose. Fifty seven percent of oocytes in SDZ medium consumed all available glucose.
Conclusions: Gene expression changed upon in vitro maturation and gonadotropin stimulation. Higher glucose availability might be needed during IVM.
Implications: This is the first study examining GC gene expression and COC metabolic requirements in rhinoceros, which are critical aspects to optimise IVM of rhinoceros oocytes.
Keywords: assisted reproductive technologies, endangered species, granulosa cell, in vitro maturation, oocyte, ovum pickup, reproduction, rhinoceros.
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