Bioinformatic prediction of the structure and characteristics of human sperm acrosome membrane-associated protein 1 (hSAMP32) and evaluation of its antifertility function in vivo
Tianwu Zhang A * , Junmin Wang B * , Wenbin Niu C , Fang Wang C , Jin Liu B , Yinpei Xing B , Peijun Jia B , Xiuhua Ren B , Jiarui Wang D , Weidong Zang B and Xuemei Chen
B E
+ Author Affiliations
- Author Affiliations
A School of Computer Science, Henan University of Engineering, Xinzheng, Henan Province, 451191, China.
B Department of Human Anatomy, Basic Medical College of Zhengzhou University, Zhengzhou, Henan Province, 450001, China.
C Reproductive Medical Center of the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, 450052, China.
D School of Public Health, Johns Hopkins University, Baltimore, MD 21218, USA.
E Corresponding author. Email: xxchxm@163.com
Reproduction, Fertility and Development 32(16) 1282-1292 https://doi.org/10.1071/RD20198
Submitted: 4 August 2020 Accepted: 8 October 2020 Published: 23 November 2020
Abstract
Human sperm acrosome membrane-associated protein 1 (hSAMP32) plays an important role in the acrosome reaction, sperm–egg primary binding, secondary binding and fusion processes. However, its spatial structural and in vivo antifertility function remain unknown. In this study, we first analysed the physical and chemical characteristics and antigenic epitopes of immunised mice using bioinformatics. Then, we constructed the prokaryotic expression vector pcDNA3.1-hSAMP32 to immunise BALB/c mice in vivo. IgG antibodies in the serum were detected, and the litter size of female mice and the number of the hamster eggs penetrated were counted. hSAMP32 was found to contain six hydrophilic regions and a signal peptide beginning at amino acid position 29. The transmembrane region of hSAMP32 was located within amino acids 217–239 with α-helices and random coil structures. We predicted five antigenic epitopes. The molecular weight of hSAMP32 was 59 kDa. Moreover, the results of in vivo studies revealed that 56 days after the first immunisation, the litter size was significantly smaller for female pcDNA-3.1(+)-hSAMP32-immunised (mean ± s.d. 4.33 ± 1.21) than control mice (9.50 ± 0.55), indicating that the immunocontraception vaccine had an antifertility effect. This experiment presents a theoretical and experimental basis for in-depth study of the hSAMP32 mechanism within the sperm-egg fusing process and for the screening of antigenic epitopes with immunocontraceptive properties.
Keywords: bioinformatics, Homo sapiens sperm acrosome associated 1 (hSPACA1), human sperm acrosome membrane-associated protein 1 (hSAMP32), immune infertility, immunocontraception, protein expression, protein structure.
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