Distribution and size of lipid droplets in oocytes recovered from young lamb and adult ovine ovaries
Amirhossein Abazarikia
A B D , Federica Ariu B , Mahsa Rasekhi C , Mahdi Zhandi A and Sergio Ledda B
+ Author Affiliations
- Author Affiliations
A Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj 31587-77871, Iran.
B Department of Veterinary Medicine, Section of Obstetrics and Gynecology, University of Sassari, Sassari, Italy.
C Department of Animal and Marine Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran 1497716316, Iran.
D Corresponding author. Email: abazarikia1984@gmail.com
Reproduction, Fertility and Development 32(11) 1022-1026 https://doi.org/10.1071/RD20035
Submitted: 2 February 2020 Accepted: 2 June 2020 Published: 7 July 2020
Abstract
This study evaluated the distribution and size of lipid droplets (LDs) in oocytes recovered from young and adult ovine ovaries. Collected oocytes were categorised on the basis of their major diameter (small (SO), 70–90 µm; medium (MO), >90–110 µm; large (LO), >110–130 µm) and were stained with Nile red to detect LDs. In adult and young oocytes, a diffuse pattern distribution of LDs was dominant in all classes except adult LO and young SO and LO. Larger LDs (i.e. >3 µm) were mostly present in young SO and LO, whereas smaller LDs (1–3 µm) were detected in the other adult and young oocyte categories.
Additional keywords: growth, livestock.
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