Morphometric, subcellular, in vitro fertilisation and embryonic developmental assessment of mouse oocytes produced by anti-inhibin serum or pregnant mare serum gonadotrophin superovulation
Liga Wuri
A , Cansu Agca B and Yuksel Agca A B C
+ Author Affiliations
- Author Affiliations
A Division of Animal Sciences, University of Missouri, 920 East Campus Drive, Columbia, MO 65201, USA.
B College of Veterinary Medicine, University of Missouri, 1600 East Rollins Street, Columbia, MO 65211, USA.
C Corresponding author. Email: agcay@missouri.edu
Reproduction, Fertility and Development 32(5) 474-483 https://doi.org/10.1071/RD19131
Submitted: 7 April 2019 Accepted: 1 August 2019 Published: 24 January 2020
Abstract
This study compared the morphometric, subcellular characteristics, in vitro fertilisation (IVF) and embryonic developmental potential of metaphase II (MII) mouse oocytes obtained from females superovulated with either anti-inhibin serum–human chorionic gonadotrophin (AIS-hCG) or pregnant mare serum gonadotrophin (PMSG)-hCG. The oocyte’s quantity, quality, zona pellucida (ZP) thickness, perivitelline space (PVS), diameter, microtubules, F-actin, cortical granules (CGs) and mitochondrial distribution were determined. Superovulation using AIS-hCG resulted in a higher numbers of oocyte/donor compared with PMSG-hCG (P = 0.002). There was no difference in morphologically normal and abnormal oocytes between AIS-hCG and PMSG-hCG (P = 0.425 and P = 0.194, respectively). The morphometric measurements showed no difference in oocyte diameter between AIS-hCG and PMSG-hCG (P = 0.289). However, the thickness of the ZP of oocytes from AIS-hCG females was decreased compared with PMSG-hCG (P < 0.001). The PVS of oocytes from the AIS-hCG was larger than with PMSG-hCG (P < 0.001). The microtubules of oocytes from both AIS-hCG and PMSG-hCG were normal, although there was an increased fluorescence intensity in the AIS-hCG oocytes (P < 0.001). The F-actin and CGs distribution in oocytes from both AIS-hCG and PMSG-hCG were similar (P = 0.330 and P = 0.13, respectively). Although the oocytes from PMSG-hCG females had homogenously distributed mitochondria, AIS-hCG oocytes showed more peripheral distribution with no differences in fluorescence intensity (P = 0.137). The blastocyst development rates after IVF with fresh sperm showed no difference between AIS-hCG and PMSG-hCG (P = 0.235). These data suggested that AIS-hCG superovulation produces high numbers of morphologically normal oocytes that also possess normal subcellular structures, good morphological characteristics and had high in vitro embryonic developmental potential.
Additional keywords: cortical granules, F-actin, microtubules, mitochondria, zona pellucida.
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