Cytoplasm lipids can be modulated through hormone-sensitive lipase and are related to mitochondrial function in porcine IVM oocytes
Qingrui Zhuan A * , Haojia Ma A * , Jing Chen A * , Yuxi Luo A , Yan Luo B , Lei Gao A , Yunpeng Hou B , Shien Zhu A and Xiangwei Fu
A C
+ Author Affiliations
- Author Affiliations
A National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Haidian District, Beijing 100193, China.
B State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Haidian District, Beijing 100193, China.
C Corresponding author. Email: xiangweifu@126.com
Reproduction, Fertility and Development 32(7) 667-675 https://doi.org/10.1071/RD19047
Submitted: 4 February 2019 Accepted: 14 November 2019 Published: 16 March 2020
Abstract
Intracellular lipids provide energy for oocyte maturation and development. Triglycerides are the main components of cytoplasm lipid droplets, and hydrolysis of triglycerides requires several lipase-mediated steps. The aim of this study was to determine the effects of the β-adrenoceptor agonist isoproterenol (ISO) and the hormone-sensitive lipase (HSL) inhibitor CAY10499 on the IVM of porcine oocytes. ISO (5 mg L−1) and CAY10499 (20 mg L−1) had positive and negative effects respectively on in vitro oocyte maturation and subsequent embryo development. The rates of polar body extrusion, cleavage and blastocyst formation were significantly higher in the ISO-treated group than the control and CAY10499-treated groups. ISO treatment also upregulated intracellular cAMP levels in comparison with the control group, while CAY10499 significantly increased the triglyceride content of matured oocytes when compared with other groups, consistent with the observed decrease in LIPE (HSL) mRNA levels. Furthermore, the inhibitory effects of CAY10499 included decreases in mitochondrial membrane potential and mitochondrial temperature. These results indicate that ISO has a positive effect on the IVM of porcine oocytes, and that intracellular lipid metabolism can be modulated by CAY10499 through inhibition of HSL and is closely related to mitochondrial function.
Additional keywords: lipid content, mitochondria, triglyceride.
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