Variations in the adenohypophysis of the expression of proliferating cellular nuclear antigen, oestrogen and androgen receptors in relation to gonadal steroids during pregnancy of viscachas (Lagostomus maximus maximus)
Gabriela J. Rosales A B , Edith Perez A , Graciela B. Rodriguez C , Verónica P. Filippa
A B D and Fabian H. Mohamed A
+ Author Affiliations
- Author Affiliations
A Laboratorio de Histología, Área Morfología, Departamento de Bioquímica y Ciencias Biológicas, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Avenida Ejército de los Andes 950 Bloque I Piso 1°, 5700 San Luis, Argentina.
B Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 5700 San Luis, Argentina.
C Laboratorio de Parasitología, Área de Análisis Clínicos, Departamento de Bioquímica y Ciencias Biológicas, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Avenida Ejercito de los Andes 950, Bloque I, Piso 1°, 5700 San Luis, Argentina.
D Corresponding author. Email: vpfilipp@unsl.edu.ar
Reproduction, Fertility and Development 31(11) 1707-1718 https://doi.org/10.1071/RD18423
Submitted: 24 October 2018 Accepted: 27 May 2019 Published: 27 June 2019
Abstract
Viscachas are native rodents of South America that present a long pregnancy of ~154 days. In this work, we analysed variations in the expression of proliferating cellular nuclear antigen, oestrogen and androgen receptors (ERα and AR) in pituitary pars distalis (PD) and pars tuberalis (PT) in relation to oestradiol and testosterone serum levels in non-pregnant and pregnant viscachas. In PD, cell proliferation increased with pregnancy and lactotrophs proliferated during mid-pregnancy (MP). ERα nuclear-immunoreactive cells (ERαn-ir) were maximal in late pregnancy and AR expression did not vary during pregnancy. In PT, cell proliferation and AR expression increased during pregnancy, but ERα expression was very scarce. The immunostaining pattern of receptors was different in PD and PT. The peak of serum oestradiol and testosterone occurred during MP. Our results suggest that cell proliferation and gonadal receptors might be differentially regulated in the pituitary by oestradiol and testosterone during viscacha pregnancy.
Additional keywords: gestation, hormone receptor, pituitary, proliferation, rodent.
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