MRL/MpJ mice produce more oocytes and exhibit impaired fertilisation and accelerated luteinisation after superovulation treatment
Marina Hosotani
A , Osamu Ichii A , Teppei Nakamura A B , Md Abdul Masum A , Yuki Otani A , Saori Otsuka-Kanazawa A , Yaser H. A. Elewa A C and Yasuhiro Kon A D
+ Author Affiliations
- Author Affiliations
A Laboratory of Anatomy, Division of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-Ku, Sapporo, Hokkaido 060-0818, Japan.
B Section of Biological Safety Research, Chitose Laboratory, Japan Food Research Laboratories, Bunkyo 2–3, Chitose, Hokkaido 066-0052, Japan.
C Department of Histology and Cytology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.
D Corresponding author. Email: y-kon@vetmed.hokudai.ac.jp
Reproduction, Fertility and Development 31(4) 760-773 https://doi.org/10.1071/RD18319
Submitted: 13 April 2018 Accepted: 9 November 2018 Published: 4 December 2018
Abstract
MRL/MpJ mice exhibit distinct phenotypes in several biological processes, including wound healing. Herein we report two unique phenotypes in the female reproductive system of MRL/MpJ mice that affect ovulation and luteinisation. We found that superovulation treatment resulted in the production of significantly more oocytes in MRL/MpJ than C57BL/6 mice (71.0 ± 13.4 vs 26.8 ± 2.8 respectively). However, no exon mutations were detected in genes coding for female reproductive hormones or their receptors in MRL/MpJ mice. In addition, the fertilisation rate was lower for ovulated oocytes from MRL/MpJ than C57BL/6 mice, with most of the fertilised oocytes showing abnormal morphology, characterised by deformation and cytolysis. Histological tracing of luteinisation showed that MRL/MpJ mice formed corpora lutea within 36 h after ovulation, whereas C57BL/6 mice were still at the corpora haemorrhagica formation stage after 36 h. The balance between the expression of matrix metalloproteinases and their tissue inhibitors shifted towards the former earlier after ovulation in MRL/MpJ than C57BL/6 mice. This result indicates a possible link between accelerated extracellular matrix remodelling in the ovulated or ruptured follicles and luteinisation in MRL/MpJ mice. Together, these findings reveal novel phenotypes in MRL/MpJ mice that provide novel insights into reproductive biology.
Additional keywords: extracellular matrix, histology, in vitro fertilisation, ovary.
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