CASA-Mot technology: how results are affected by the frame rate and counting chamber
Daznia Bompart A E , Almudena García-Molina A , Anthony Valverde B C , Carina Caldeira A B , Jesús Yániz D , Manuel Núñez de Murga B and Carles Soler A B
+ Author Affiliations
- Author Affiliations
A Proiser R+D, Avenuenida Catedrático Agustín Escardino, 9, Building 3 (CUE), Floor 1, 46980, Paterna, Spain.
B University of Valencia, Department of Cellular Biology, Functional Biology and Physical Anthropology, Campus Burjassot, C/ Dr Moliner, 50, 46100, Burjassot, Spain.
C Technological Institute of Costa Rica, San Carlos Campus, School of Agronomy, 223-21001 Alajuela, Costa Rica.
D TECNOGAM Research Group, Environmental Sciences Institute (IUCA), Department of Animal Production and Food Sciences, University of Zaragoza, 50013, Huesca, Spain.
E Corresponding author. Email: daznia.bompart@proiser.com
Reproduction, Fertility and Development 30(6) 810-819 https://doi.org/10.1071/RD17551
Submitted: 25 October 2017 Accepted: 18 January 2018 Published: 4 April 2018
Journal Compilation © CSIRO 2018 Open Access CC BY-NC-ND
Abstract
For over 30 years, CASA-Mot technology has been used for kinematic analysis of sperm motility in different mammalian species, but insufficient attention has been paid to the technical limitations of commercial computer-aided sperm analysis (CASA) systems. Counting chamber type and frame rate are two of the most important aspects to be taken into account. Counting chambers can be disposable or reusable, with different depths. In human semen analysis, reusable chambers with a depth of 10 µm are the most frequently used, whereas for most farm animal species it is more common to use disposable chambers with a depth of 20 µm . The frame rate was previously limited by the hardware, although changes in the number of images collected could lead to significant variations in some kinematic parameters, mainly in curvilinear velocity (VCL). A frame rate of 60 frames s−1 is widely considered to be the minimum necessary for satisfactory results. However, the frame rate is species specific and must be defined in each experimental condition. In conclusion, we show that the optimal combination of frame rate and counting chamber type and depth should be defined for each species and experimental condition in order to obtain reliable results.
Additional keywords: computer-aided sperm analysis (CASA) system, kinematic, motility.
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