Hypothyroidism affects lipid and glycogen content and peroxisome proliferator-activated receptor δ expression in the ovary of the rabbit
Julia Rodríguez-Castelán A , Maribel Méndez-Tepepa A , Jorge Rodríguez-Antolín B , Francisco Castelán B C D and Estela Cuevas-Romero B D
+ Author Affiliations
- Author Affiliations
A Doctorado en Ciencias Biológicas, Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, 90070, México.
B Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México, 90070.
C Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Unidad Foránea Tlaxcala, Universidad Nacional Autónoma de México, Tlaxcala, 90070, México.
D Corresponding authors. Emails: ecuevas@uatx.mx; fcocastelan@iibiomedicas.unam.mx
Reproduction, Fertility and Development 30(10) 1380-1387 https://doi.org/10.1071/RD17502
Submitted: 12 October 2017 Accepted: 27 March 2018 Published: 3 May 2018
Abstract
Dyslipidaemia and hyperglycaemia are associated with ovarian failure and both have been related to hypothyroidism. Hypothyroidism promotes anovulation and ovarian cysts in women and reduces the size of follicles and the expression of aromatase in the ovary of rabbits. Considering that ovarian steroidogenesis and ovulation depend on lipid metabolism and signalling, the aim of the present study was to analyse the effect of hypothyroidism on the lipid content and expression of peroxisome proliferator-activated receptor (PPAR) δ in the ovary. Ovaries from female rabbits belonging to the control (n = 7) and hypothyroid (n = 7) groups were processed to measure total cholesterol (TC), triacylglycerol (TAG) and glycogen content, as well as to determine the presence of granules containing oxidized lipids (oxysterols and lipofuscin) and the relative expression of perilipin A (PLIN-A) and PPARδ. Hypothyroidism increased TC and glycogen content, but reduced TAG content in the ovary. This was accompanied by a reduction in the expression of PLIN-A in total and cytosolic extracts, changes in the presence of granules containing oxidative lipids and low PPARδ expression. The results of the present study suggest that hypothyroidism modifies the content and signalling of lipids in the ovary, possibly affecting follicle maturation. These results could improve our understanding of the association between hypothyroidism and infertility in females.
Additional keywords: oogenesis, ovary metabolism, ovulation, reproduction.
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