Nitric oxide activation by progesterone suppresses ATP-induced ciliary activity in oviductal ciliated cells
Bredford Kerr A D , Mariana Ríos B , Karla Droguett C and Manuel Villalón C D
+ Author Affiliations
- Author Affiliations
A Centro de Estudios Científicos, Av. Arturo Prat 514, 5110466, Valdivia, Chile.
B Millenium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, 8331150 Santiago, Chile.
C Departamento de Ciencias Fisiológicas, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, 8331150 Santiago, Chile.
D Corresponding authors. Emails: bkerr@cecs.cl; mvillalon@bio.puc.cl
Reproduction, Fertility and Development 30(12) 1666-1674 https://doi.org/10.1071/RD17450
Submitted: 26 October 2017 Accepted: 7 May 2018 Published: 25 June 2018
Abstract
Ciliary beat frequency (CBF) regulates the oviductal transport of oocytes and embryos, which are important components of the reproductive process. Local release of ATP transiently increases CBF by increasing [Ca2+]i. Ovarian hormones also regulate ciliary activity and oviductal transport. Progesterone (P4) induces nitric oxide (NO) production and high P4 concentrations induce ciliary dysfunction. However, the mechanism by which P4 affects CBF has not been elucidated. To evaluate the role of P4 in NO production and its effect on ATP-induced increases in CBF, we measured CBF, NO concentrations and [Ca2+]i in cultures of oviductal ciliated cells treated with P4 or NO signalling-related molecules. ATP induced a [Ca2+]i peak, followed by an increase in NO concentrations that were temporally correlated with the decreased phase of the transiently increased CBF. Furthermore, P4 increased the expression of nitric oxide synthases (iNOS and nNOS) and reduced the ATP-induced increase in CBF via a mechanism that involves the NO signalling pathway. These results have improved our knowledge about intracellular messengers controlling CBF and showed that NO attenuates oviduct cell functions. Furthermore, we showed that P4 regulates neurotransmitter (ATP) actions on CBF via the NO pathway, which could explain pathologies where oviductal transport is altered and fertility decreased.
Additional keywords: ciliary beat frequency, free intracellular Ca2+, oviduct.
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