Successful chimera production in the Hungarian goose (Anser anser domestica) by intracardiac injection of blastodermal cells in 3-day-old embryos
Nikoletta Sztán A , Bence Lázár A B , Nóra Bodzsár A , Barbara Végi A , Krisztina Liptói A , Bertrand Pain C and Eszter Patakiné Várkonyi A D
+ Author Affiliations
- Author Affiliations
A Research Centre for Farm Animal Gene Conservation, Isaszegi Street 200, Gödöllő, H-2100, Hungary.
B National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Szent Györgyi Albert Street 4, H-2100, Hungary.
C U1208, INSERM, USC1361, INRA, Stem Cell and Brain Research Institute, 18, Ae Doyen Lépine, 69500 Bron, France.
D Corresponding author. Email: varkonyi.eszter@hagk.hu
Reproduction, Fertility and Development 29(11) 2206-2216 https://doi.org/10.1071/RD16289
Submitted: 26 July 2016 Accepted: 14 February 2017 Published: 27 March 2017
Abstract
The conservation of genetic resources of avian species has become increasingly important over the past decade. The aim of the present study was to develop a genome preservation technique for the Hungarian goose Anser anser domestica. To this end, we developed a novel approach combining the simplicity of isolating a blastodermal cell suspension, which includes forming primordial germ cells (PGCs), with the efficiency of targeting future gonads by injecting these cells into the cardiac vein of the developing host embryo. First, we determined that the migratory period of PGCs in goose embryos was between 69 and 84 h of development. Then, we injected the blastodermal cell suspension into the bloodstream of recipient embryos at this stage of development and monitored donor cell transmission into the genital tract. In all, 249 embryos were injected; three were found to be chimeras in gonadal tissues, whereas only one was a chimera in other tissues. Based on these results, it is concluded that this method is suitable for producing chimeras in the domestic goose. The optimal time of cell injection was found to be between 74 and 76 h. The present study is the first report of the generation of chimeras in the domestic goose using intracardiac transplantation of embryonic cells.
Additional keywords: developmental stages, embryonic stem cell; genetic conservation, waterfowl.
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