Aquaporins in boar spermatozoa. Part II: detection and localisation of aquaglyceroporin 3
Noelia Prieto-Martínez A D , Roser Morató A , Ingrid Vilagran A , Joan E. Rodríguez-Gil B , Sergi Bonet A and Marc Yeste C
+ Author Affiliations
- Author Affiliations
A Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, E-17071 Girona, Spain.
B Unit of Animal Reproduction, Department of Animal Medicine and surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra (Barcelona), Spain.
C Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Level 3, Women’s Centre, John Radcliffe Hospital, Headington, Oxford, OX3 9DU, UK.
D Corresponding author. Email: noelia.prieto@udg.edu
Reproduction, Fertility and Development 29(4) 703-711 https://doi.org/10.1071/RD15164
Submitted: 26 April 2015 Accepted: 26 October 2015 Published: 18 December 2015
Abstract
The proteins belonging to the aquaporin family play a fundamental role in water and solute transport across biological membranes. While the presence of these proteins has been extensively studied in somatic cells, their function in mammalian spermatozoa has been studied less. The present study was designed to identify and localise aquaglyceroporin 3 (AQP3) in boar spermatozoa. With this purpose, 29 fresh ejaculates from post-pubertal Piétrain boars were classified into two groups based upon their sperm quality and subsequently evaluated through western blot and immunofluorescence assessments. Western blotting showed the specific signal band of AQP3 at 25 kDa, whereas immunofluorescence assessments allowed us to identify two different AQP3 localisation patterns: (1) spermatozoa presenting a clear labelling located only in the mid-piece and (2) spermatozoa exhibiting a distribution pattern in the head and along the entire tail. The first staining pattern was predominant in all studied ejaculates. Despite individual differences in AQP3 content and localisation between boar ejaculates, these differences were not correlated with sperm quality. In conclusion, although AQP3 is present in boar spermatozoa in two different localisation patterns, neither the AQP3 content nor its localisation have been found to be associated with conventional sperm parameters.
Additional keywords: aquaglyceroporin 3, boar sperm, mid-piece, sperm tail, western blot.
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