Expression and function of transcription factor AP-2γ in early embryonic development of porcine parthenotes
Sung-Hyun Lee A , Jung-Woo Kwon A , Inchul Choi B C and Nam-Hyung Kim A C
+ Author Affiliations
- Author Affiliations
A Department of Animal Sciences, Chungbuk National University, Gaesin-dong, Cheongju, Chungbuk 361-763, Republic of Korea.
B Department of Animal Biosystem Sciences, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Republic of Korea.
C Corresponding authors. Email: nhkim@chungbuk.ac.kr; icchoi@cnu.ac.kr
Reproduction, Fertility and Development 28(8) 1197-1205 https://doi.org/10.1071/RD14198
Submitted: 9 June 2014 Accepted: 3 December 2014 Published: 7 January 2015
Abstract
Transcription factor AP-2γ (TFAP2C) is a member of the transcription factor activating enhancer binding protein (AP) family. In the present study we determined the temporal and spatial expression patterns of TFAP2C in porcine parthenotes during preimplantation development. Porcine TFAP2C transcripts were expressed at all stages of preimplantation development, with highest expression at the 8-cell stage. In contrast with the mouse, TFAP2C protein was not restricted to the trophectoderm and was also detected in the ICM in blastocyst stage porcine parthenotes. In knockdown (KD) experiments, most TFAP2C-depleted embryos were arrested before the compacted 8-cell stage. This developmental failure is attributed to abnormal expression of genes involved in cell adhesion, tight junction biogenesis and cell proliferation. Interestingly, although the conserved region 4 (CR4) of the porcine OCT4 5′ upstream regionlacked the AP2C-binding motif, OCT4 transcript levels were elevated in porcine TFAP2C-KD 8-cell embryos, suggesting TFAP2C may be involved in the regulation of OCT4 in porcine embryos through other mechanisms. In summary, the results suggest that TFAP2C is necessary for the transition from de novo transcript synthesis by activation to compaction and further development, and the different expression patterns of TFAP2C in porcine embryos may reflect species-specific functions during preimplantation embryo development.
Additional keywords: cell adhesion, cell polarity, compaction, pig embryos.
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