Tolerance of spermatozoa to hypotonic stress: role of membrane fluidity and correlation with cryosurvival
Harriëtte Oldenhof A F , Anna Heutelbeck A , Anne-Kathrin Blässe B , Heinrich Bollwein B E , Gunilla Martinsson C , Willem F. Wolkers D and Harald Sieme A
+ Author Affiliations
- Author Affiliations
A Clinic for Horses – Unit for Reproductive Medicine, University of Veterinary Medicine Hannover, Bünteweg 15, D-30559 Hannover, Germany.
B Clinic for Cattle, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, D-30173 Hannover, Germany.
C National Stud Lower Saxony, Spörckenstraße 10, D-29221 Celle, Germany.
D Institute of Multiphase Processes, Leibniz Universität Hannover, Callinstrasse 36, D-30167 Hannover, Germany.
E Present address: Clinic of Reproductive Medicine, Vetsuisse-Faculty, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, Switzerland.
F Corresponding author. Email: harriette.oldenhof@tiho-hannover.de
Reproduction, Fertility and Development 27(2) 285-293 https://doi.org/10.1071/RD13177
Submitted: 6 June 2013 Accepted: 3 October 2013 Published: 11 November 2013
Abstract
The aim of this study was to evaluate inter-individual variability in osmotic properties of stallion spermatozoa and its correlation with cryosurvival. In addition, temperature dependency of hypo-osmotic tolerance and membrane fluidity were studied. Stallion sperm membranes exhibited good resistance towards hypotonic stress in the 15–30°C temperature range, whereas membrane stability was found to be decreased at 4 and 37°C. Bull spermatozoa showed greater hypo-osmotic tolerance compared with stallion spermatozoa, especially at temperatures above 30°C, which coincided with decreased membrane fluidity of bovine spermatozoa in this temperature range. The critical osmolality at 22°C, at which half of the sperm population survived exposure to hypotonic saline solution, was found to vary between 55 and 170 mOsm kg–1 among different stallions. Clear correlations were found for pre- versus post-freeze sperm motility and membrane integrity. Pre-freeze percentages of membrane-intact spermatozoa after exposure to hypotonic stress showed a weak correlation with sperm motility after cryopreservation. This correlation, however, was not found when data were corrected for initial numbers of membrane-intact spermatozoa in the sample. We thus conclude that studies on pre-freeze tolerance towards hypotonic stress cannot be used to predict sperm cryosurvival rates for individual stallions.
Additional keywords: cryopreservation, osmotic tolerance, stallion spermatozoa.
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