Global gene expression in the bovine corpus luteum is altered after stimulatory and superovulatory treatments
Luciana A. Fátima A E , Pietro S. Baruselli B , Lindsay U. Gimenes B , Mario Binelli B , Francisco P. Rennó C , Bruce D. Murphy D and Paula C. Papa A
+ Author Affiliations
- Author Affiliations
A Sector of Anatomy, Department of Surgery, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo – SP, 05508-270 Brazil.
B Department of Animal Reproduction, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga – SP, 13635-900, Brazil.
C Department of Nutrition and Animal Production, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga – SP, 13635-900, Brazil.
D Animal Reproduction Research Centre (CRRA), University of Montreal, St-Hyacinthe, QC J2S 7C6, Canada.
E Corresponding author. Email: lubiologia2000@yahoo.com.br
Reproduction, Fertility and Development 25(7) 998-1011 https://doi.org/10.1071/RD12155
Submitted: 17 May 2012 Accepted: 5 September 2012 Published: 30 October 2012
Abstract
Equine chorionic gonadotrophin (eCG) has been widely used in superovulation and artificial insemination programmes and usually promotes an increase in corpus luteum (CL) volume and stimulates progesterone production. Therefore, to identify eCG-regulated genes in the bovine CL, the transcriptome was evaluated by microarray analysis and the expression of selected genes was validated by qPCR and western blot. Eighteen Nelore crossbred cows were divided into control (n = 5), stimulated (n = 6) and superovulated groups (n = 7). Ovulation was synchronised using a progesterone device-based protocol. Stimulated animals received 400 IU of eCG at device removal and superovulated animals received 2000 IU of eCG 4 days prior. Corpora lutea were collected 7 days after gonadotrophin-releasing hormone administration. Overall, 242 transcripts were upregulated and 111 transcripts were downregulated in stimulated cows (P ≤ 0.05) and 111 were upregulated and 113 downregulated in superovulated cows compared to the control animals (1.5-fold, P ≤ 0.05). Among the differentially expressed genes, many were involved in lipid biosynthesis and progesterone production, such as PPARG, STAR, prolactin receptors and follistatin. In conclusion, eCG modulates gene expression differently depending on the treatment, i.e. stimulatory or superovulatory. Our data contribute to the understanding of the pathways involved in increased progesterone levels observed after eCG treatment.
Additional keywords : eCG, follistatin, lipid metabolism, progesterone, prolactin receptors, PPARG.
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