Levels of mRNA for bone morphogenetic proteins, their receptors and SMADs in goat ovarian follicles grown in vivo and in vitro
J. J. N. Costa A , M. J. Passos A , C. C. F. Leitão A , G. L. Vasconcelos A , M. V. A. Saraiva C , J. R. Figueiredo C , R. van den Hurk B and J. R. V. Silva A D
+ Author Affiliations
- Author Affiliations
A Biotechnology Nucleus of Sobral – NUBIS, Federal University of Ceara, CEP 62042-280, Sobral, CE, Brazil.
B Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.163, Utrecht, The Netherlands.
C LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceara, CEP 60740-000, Fortaleza, CE, Brazil.
D Corresponding author. Email: jrvsilva@ufc.br
Reproduction, Fertility and Development 24(5) 723-732 https://doi.org/10.1071/RD11195
Submitted: 5 August 2011 Accepted: 31 October 2011 Published: 13 December 2011
Abstract
This study investigated the stability of housekeeping genes (glyceraldehyde-3-phosphate dehydrogenase, β-tubulin, β-actin, phosphoglycerate kinase (PGK), 18S rRNA, ubiquitin and ribosomal protein 19) and the levels of mRNA for bone morphogenetic protein-2 (BMP-2), -4 (BMP-4), -6 (BMP-6), -7 (BMP-7) and -15 (BMP-15), their receptors (BMPR-IA, -IB and -II) and Similar to Mothers Against Decapentaplegic (SMADs) (-1, -5 and -8) in goat follicles of 0.2, 0.5 and 1.0 mm, as well as in secondary follicles before and after culture for 18 days. β-tubulin and PGK were the most stable housekeeping genes and the levels of mRNA for BMP-2 in follicles of 0.2 mm were higher than in follicles of 0.5 and 1.0 mm. For BMP-4, -6 and -7, the highest levels of mRNA were found in follicles of 1.0 mm. The expression of BMPR-IB was higher in follicles of 0.2 mm, whereas the levels of BMPR-II were higher in follicles of 0.5 mm. The levels of mRNA for SMAD-5 were higher in follicles of 0.2 mm, whereas SMAD-8 had higher levels in 0.5-mm follicles. After culture, follicles showed increased levels of mRNA for BMP-2 and reduced mRNA for BMP-4, BMP-7, BMPR-IA and SMAD-5. In conclusion, β-tubulin and PGK are the most stable reference genes, and BMPs, their receptors and SMADs have variable levels of mRNA in the follicular size classes analysed.
Additional keywords: BMPs, housekeeping genes, ovary.
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