Booroola BMPR1B mutation alters early follicular development and oocyte ultrastructure in sheep
Karen L. Reader A E , Lisa J. Haydon A , Roger P. Littlejohn B D , Jennifer L. Juengel A and Kenneth P. McNatty C
+ Author Affiliations
- Author Affiliations
A AgResearch, Animal Production, Invermay Agricultural Centre, Private Bag 50034, Mosgiel 9053, New Zealand.
B Bioinformatics, Mathematics and Statistics, Invermay Agricultural Centre, Private Bag 50034, Mosgiel 9053, New Zealand.
C Victoria University of Wellington, School of Biological Sciences, PO Box 600, Wellington 6140, New Zealand.
D Dr Roger Littlejohn died on 6 March 2011.
E Corresponding author. Email: karen.reader@agresearch.co.nz
Reproduction, Fertility and Development 24(2) 353-361 https://doi.org/10.1071/RD11095
Submitted: 14 April 2011 Accepted: 18 July 2011 Published: 2 November 2011
Abstract
Booroola ewes homozygous (BB) for a mutation in the bone morphogenetic protein receptor-1b (BMPR1B) gene exhibit higher ovulation rates, have larger diameter oocytes at earlier stages of follicular development (i.e. Type 3) and smaller diameter follicles at ovulation than wild-type (++) sheep. However, it is not known when BMPR1B is first expressed in the developing ovary or the cell types involved. In addition, the effects of the BMPR1B mutation on primordial (Type 1) follicles or during growth to the Type 3 stage are unknown. In the present study, BB and ++ fetal ovaries at Days 30–135 of gestation were screened by in situ hybridisation for BMPR1B mRNA. Ovaries from BB and ++ lambs were examined by microscopy to measure follicular and oocyte ultrastructural characteristics in Type 1–3 follicles. BMPR1B mRNA was observed in ovaries from Day 35 of gestation and was evident in oocytes of newly forming and fully formed Type 1 follicles. In BB animals, the Type 1 follicles had larger mean follicular and oocyte diameters, a greater volume of mitochondria, smooth endoplasmic reticulum and ribosomes and a greater surface area of junctions with the granulosa cells compared with ++ animals. It is concluded that the BMPR1B mutation alters follicular development from the onset of follicular formation.
Additional keywords: BMPR1B mRNA expression, electron microscopy, mitochondria, ovarian development, ribosomes, smooth endoplasmic reticulum.
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