A polymorphism at the melatonin receptor 1A (MTNR1A) gene in Sarda ewes affects fertility after AI in the spring
Vincenzo Carcangiu A B , Sebastiano Luridiana A , Giuseppe Massimo Vacca A , Cinzia Daga A and Maria Consuelo Mura A
+ Author Affiliations
- Author Affiliations
A Dipartimento di Biologia Animale, Università degli Studi di Sassari, Via Vienna 2, 07100, Sassari, Italy.
B Corresponding author. Email: endvet@uniss.it
Reproduction, Fertility and Development 23(2) 376-380 https://doi.org/10.1071/RD10014
Submitted: 26 January 2010 Accepted: 23 August 2010 Published: 4 January 2011
Abstract
The effect of MTNR1A gene polymorphisms on the fertility rate after AI in Sarda sheep was evaluated in 600 lactating adult ewes. Genomic DNA was subjected to amplification of the MTNR1A gene exon II. Amplicons were digested with restriction endonuclease MnlI. Ten samples from each genotype were sequenced. A polymorphism was detected (A612G) and ewes were determined to be +/+, +/– or –/– for the allele. Allelic frequency was 0.77 for the + allele and 0.23 for the – allele. The frequency of the +/+, +/– and –/– genotypes was 68, 19 and 13%, respectively. On 16 May 2009, 60 ewes from each genotype group were synchronised using intravaginal sponges containing 40 mg fluorogestone acetate for 14 days. At sponge removal, the ewes were administered 350 IU pregnant mare’s serum gonadotropin and were then inseminated, 54–56 h later, with 400 × 106 spermatozoa. Pregnancies were confirmed 50 days after AI using transabdominal ultrasonography. Lambing dates and the number of newborn lambs were recorded within 155 days after AI. Conception and lambing rate were higher for ewes with the +/+ and +/– genotypes compared with those with the –/– genotype (P < 0.01). In conclusion, there was a positive correlation between MTNR1A allele polymorphisms the reproductive response following synchronisation and AI in the spring.
Additional keywords: conception rate, estrus synchronisation, FGA sponges, seasonality.
References
Abecia, J. A., Forcada, F., and Zúñiga, O. (2002). The effect of melatonin on secretion of progesterone in sheep and on development of ovine embryos in vitro. Vet. Res. Commun. 26, 151–158.| The effect of melatonin on secretion of progesterone in sheep and on development of ovine embryos in vitro.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD387ovFamug%3D%3D&md5=a932bbcef5dc2cbec7cbfb42755621c0CAS | 11922484PubMed |
Anel, L., Kaabi, M., Abroug, B., Alvarez, M., Anel, E., Boixo, J. C., de la Fuente, L. F., and de Paz, P. (2005). Factors influencing the success of vaginal and laparoscopic artificial insemination in churra ewes: a field assay. Theriogenology 63, 1235–1247.
| Factors influencing the success of vaginal and laparoscopic artificial insemination in churra ewes: a field assay.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2M%2Fot1Kksg%3D%3D&md5=21ed78622a687581dee3f3fa209740c3CAS | 15710206PubMed |
Anel, L., Alvarez, M., Martinez-Pastor, F., Garcia-Macias, V., Anel, E., and de Paz, P. (2006). Improvement strategies in ovine artificial insemination. Reprod. Domest. Anim. 41, 30–42.
| Improvement strategies in ovine artificial insemination.Crossref | GoogleScholarGoogle Scholar | 16984467PubMed |
Arendt, J. (1998). Melatonin and the pineal gland: influence on mammalian seasonal and circadian physiology. Rev. Reprod. 3, 13–22.
| Melatonin and the pineal gland: influence on mammalian seasonal and circadian physiology.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXht1WrsLY%3D&md5=81b0ce7a055b1c9be44065654adee9efCAS | 9509985PubMed |
Barrett, P., Conway, S., Jockers, R., Strosberg, A. D., Guardiola-Lemaitre, B., Delagrange, P., and Morgan, P. J. (1997). Cloning and functional analysis of a polymorphic variant of the ovine Mel 1a melatonin receptor. Biochim. Biophys. Acta 1356, 299–307.
| Cloning and functional analysis of a polymorphic variant of the ovine Mel 1a melatonin receptor.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXislehsL8%3D&md5=ffed800d25bfd19ca3fd62bad86fd7cfCAS | 9194573PubMed |
Carcangiu, V., Mura, M. C., Vacca, G. M., Pazzola, M., Dettori, M. L., Luridiana, S., and Bini, P. P. (2009). Polymorphism of the melatonin receptor MT1 gene and its relationship with seasonal reproductive activity in the Sarda sheep breed. Anim. Reprod. Sci. 116, 65–72.
| Polymorphism of the melatonin receptor MT1 gene and its relationship with seasonal reproductive activity in the Sarda sheep breed.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtVKkt7jJ&md5=6cec76c14b7c29dc45d31276554b9a98CAS | 19223130PubMed |
Carcangiu, V., Mura, M. C., Vacca, G. M., Dettori, M. L., Pazzola, M., Daga, C., and Luridiana, S. (2010). Characterization of the melatonin receptor gene MT1 in mouflon (Ovis gmelini musimon) and its relationship with reproductive activity. Mol. Reprod. Dev. 77, 196.
| Characterization of the melatonin receptor gene MT1 in mouflon (Ovis gmelini musimon) and its relationship with reproductive activity.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXotFSmsg%3D%3D&md5=cb73d04359b692fd66547d3016fcb907CAS | 19908253PubMed |
Carta, A., Casu, S., and Salaris, S. (2009). Invited review: current state of genetic improvement in dairy sheep. J. Dairy Sci. 92, 5814–5833.
| Invited review: current state of genetic improvement in dairy sheep.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhsFWisbbP&md5=4212adad84dd03f8558be2da656c3ec2CAS | 19923587PubMed |
Chabot, V., Caldani, M., de Reviers, M. M., and Pelletier, J. (1998). Localization and quantification of melatonin receptors in the diencephalon and posterior telencephalon of the sheep brain. J. Pineal Res. 24, 50–57.
| Localization and quantification of melatonin receptors in the diencephalon and posterior telencephalon of the sheep brain.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXmt1Wksg%3D%3D&md5=4af0011d3bf67a72605b1d4300f5e724CAS | 9468118PubMed |
Chemineau, P., Pellicer-Rubio, M. T., Lassoued, N., Khaldi, G., and Monniaux, D. (2006). Male-induced short estrous ovarian cycles in sheep and goats: a working hypothesis. Reprod. Nutr. Dev. 46, 417–429.
| Male-induced short estrous ovarian cycles in sheep and goats: a working hypothesis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtVOnsr3K&md5=fd1e2dcb1e9287acadbf247155a32ee0CAS | 16824450PubMed |
Chemineau, P., Guillaume, D., Migaud, M., Thiery, J. C., Pellicer-Rubio, M. T., and Malpaux, B. (2008). Seasonality of reproduction in mammals: intimate regulatory mechanisms and practical implications. Reprod. Domest. Anim. 43, 40–47.
| Seasonality of reproduction in mammals: intimate regulatory mechanisms and practical implications.Crossref | GoogleScholarGoogle Scholar | 18638103PubMed |
Chu, M. X., Cheng, D. X., Liu, W. Z., Fang, L., and Ye, S. C. (2006). Association between melatonin receptor 1A gene and expression of reproductive seasonality in sheep. Asian-Australas. J. Anim. Sci. 19, 1079–1084..
| 1:CAS:528:DC%2BD28XotlWjtr0%3D&md5=0e5349a554fe7df2acc22a959fddee43CAS |
David, I., Robert-Granie, C., Manfredi, E., Lagriffoul, G., and Bodin, L. (2008). Environmental and genetic variation factors of artificial insemination success in French dairy sheep. Animal 2, 979–986.
| Environmental and genetic variation factors of artificial insemination success in French dairy sheep.Crossref | GoogleScholarGoogle Scholar |
Dubocovich, M. L. (1988). Pharmacology and function of melatonin receptors. FASEB J. 2, 2765–2773..
| 1:CAS:528:DyaL1cXls1emtL8%3D&md5=32c837df6b89cda51514a63f5b7e7d15CAS | 2842214PubMed |
Eppleston, J., and Maxwell, W. M. C. (1995). Sources of variation in the reproductive performance of ewes inseminated with frozen–thawed ram semen by laparoscopy. Theriogenology 43, 777–788.
| Sources of variation in the reproductive performance of ewes inseminated with frozen–thawed ram semen by laparoscopy.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD28zgtVChtw%3D%3D&md5=49f8465281630b7c97c4dca42296e074CAS | 16727668PubMed |
Halbert, G. W., Dobson, H., Walton, J. S., and Buckrell, B. C. (1990). The structure of the cervical canal of the ewe. Theriogenology 33, 977–992.
| The structure of the cervical canal of the ewe.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD283pvFGmtQ%3D%3D&md5=8e15fb78ff2ec5ae94ca313f6015b7d5CAS | 16726794PubMed |
Hernandez, X., Bodin, L., Chesneau, D., Guillaume, D., Chemineau, P., Malpaux, B., and Migaud, M. (2005). Relationship between MT1 melatonin receptor gene polymorphism and seasonal physiological responses in Île-de-France ewes. Reprod. Nutr. Dev. 45, 151–162.
| Relationship between MT1 melatonin receptor gene polymorphism and seasonal physiological responses in Île-de-France ewes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXlslKisLo%3D&md5=f7123a654e935a062fa489b6d7fd85e2CAS | 15952421PubMed |
Hunter, M. G., Southee, J. A., McLeod, B. J., and Haresign, W. (1986). Progesterone pretreatment has a direct effect on GnRH-induced preovulatory follicles to determine their ability to develop into normal corpora lutea in anoestrous ewes. J. Reprod. Fertil. 76, 349–363.
| Progesterone pretreatment has a direct effect on GnRH-induced preovulatory follicles to determine their ability to develop into normal corpora lutea in anoestrous ewes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL28XhsVagu7c%3D&md5=4a961c1f4876b9e4bf7665c876f325afCAS | 3511255PubMed |
Karsch, F. J., Malpaux, B., Wayne, N. L., and Robinson, J. E. (1988). Characteristics of the melatonin signal that provide the photoperiodic code for timing seasonal reproduction in the ewe. Reprod. Nutr. Dev. 28, 459–472.
| Characteristics of the melatonin signal that provide the photoperiodic code for timing seasonal reproduction in the ewe.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1cXmt1amt7o%3D&md5=b427fe0f4672d86050e6968b5404656fCAS | 3045928PubMed |
Laliotis, V., Vosniakoua, A., Zafrakasb, A., Lymberopoulosa, A., and Alifakiotisb, T. (1998). The effect of melatonin on lambing and litter size in milking ewes after advancing the breeding season with progestagen and PMSG followed by artificial insemination. Small Rumin. Res. 31, 79–81.
| The effect of melatonin on lambing and litter size in milking ewes after advancing the breeding season with progestagen and PMSG followed by artificial insemination.Crossref | GoogleScholarGoogle Scholar |
Lincoln, G. A., and Clarke, I. J. (1994). Photoperiodically-induced cycles in the secretion of prolactin in hypothalamo-pituitary disconnected rams: evidence for translation of the melatonin signal in the pituitary gland. J. Neuroendocrinol. 6, 251–260.
| Photoperiodically-induced cycles in the secretion of prolactin in hypothalamo-pituitary disconnected rams: evidence for translation of the melatonin signal in the pituitary gland.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXksFSmsrc%3D&md5=3716b1cd9c889e94005c5f9eb655363dCAS | 7920591PubMed |
Malpaux, B., Daveau, A., Maurice-Mandon, F., Duarte, G., and Chemineau, P. (1998). Evidence that melatonin acts in the premammillary hypothalamic area to control reproduction in the ewe: presence of binding sites and stimulation of luteinizing hormone secretion by in situ microimplant delivery. Endocrinology 139, 1508–1516.
| Evidence that melatonin acts in the premammillary hypothalamic area to control reproduction in the ewe: presence of binding sites and stimulation of luteinizing hormone secretion by in situ microimplant delivery.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXitVKmtrk%3D&md5=f3d8318c2462434519a0fbf1a1dd777bCAS | 9528928PubMed |
Mateescu, R. G., Lunsford, A. K., and Thonney, M. L. (2009). Association between melatonin receptor 1A gene polymorphism and reproductive performance in Dorset ewes. J. Anim. Sci. 87, 2485–2488.
| Association between melatonin receptor 1A gene polymorphism and reproductive performance in Dorset ewes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXptV2gtr0%3D&md5=20b26daafb73424163173ed848ddf2b7CAS | 19359514PubMed |
Messer, L. A., Wang, L., Tuggle, C. K., Yerle, M., Chardon, P., Pomp, D., Womack, J. E., Barendse, W., Crawford, A. M., Notter, D. R., and Rothschild, M. F. (1997). Mapping of the melatonin receptor 1a (MTNR1A) gene in pigs, sheep and cattle. Mamm. Genome 8, 368–370.
| Mapping of the melatonin receptor 1a (MTNR1A) gene in pigs, sheep and cattle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXjtVOhsbc%3D&md5=093cd2680cea771950881518c9085797CAS | 9107687PubMed |
Notter, D. R. (2008). Genetic aspects of reproduction in sheep. Reprod. Domest. Anim. 43, 122–128.
| Genetic aspects of reproduction in sheep.Crossref | GoogleScholarGoogle Scholar | 18638113PubMed |
Notter, D. R., and Cockett, N. E. (2005). Opportunities for detection and use of QTL influencing seasonal reproduction in sheep: a review. Genet. Sel. Evol. 37, S39–S53.
| Opportunities for detection and use of QTL influencing seasonal reproduction in sheep: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXht1ens78%3D&md5=e0c966dc6804bf9895266be4e6ae234dCAS | 15601594PubMed |
Notter, D. R., Cockett, N. E., and Hadfied, T. S. (2003). Evaluation of melatonin receptor 1a as candidate gene influencing reproduction in an autumn-lambing sheep flock. J. Anim. Sci. 81, 912–917..
| 1:CAS:528:DC%2BD3sXjtFCmsb0%3D&md5=6cff0148252f823c872581e13de2f82bCAS | 12723079PubMed |
Paulenz, H., Adnøy, T., Fossen, O., and Söderquist, L. (2010). Effect on field fertility of addition of gelatine, different dilution rates and storage times of cooled ram semen after vaginal insemination. Reprod. Domest. Anim. 45, 706–710..
| 1:STN:280:DC%2BC3cnpsFSrtA%3D%3D&md5=ed96faebb0b9b1ee41f379410359b033CAS | 19220797PubMed |
Pearce, D. T., and Robinson, T. J. (1985). Plasma progesterone concentrations, ovarian and endocrinological responses and sperm transport in ewes with synchronized oestrus. J. Reprod. Fertil. 75, 49–62.
| Plasma progesterone concentrations, ovarian and endocrinological responses and sperm transport in ewes with synchronized oestrus.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2MXlslahur8%3D&md5=9fd1ea7ddb2774345094076a721bed80CAS | 3839850PubMed |
Pelletier, J., Bodin, L., Hanocq, E., Malpaux, B., Teyssier, J., Thimonier, J., and Chemineau, P. (2000). Association between expression of reproductive seasonality and alleles of the gene Mel1a receptor in the ewe. Biol. Reprod. 62, 1096–1101.
| Association between expression of reproductive seasonality and alleles of the gene Mel1a receptor in the ewe.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXitFajur4%3D&md5=1a83706ab625b7ef11b40b87beef5f24CAS | 10727283PubMed |
Reppert, S. M., Weaver, D. R., and Ebisawa, T. (1994). Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses. Neuron 13, 1177–1185.
| Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXitlylsLY%3D&md5=81682fda4b016eacaaa1357a44d90feaCAS | 7946354PubMed |
Reppert, S. M., Godson, C., Mahle, C. D., Weaver, D. R., Slaugenhaupt, S. A., and Gusella, J. F. (1995). Molecular characterization of a second melatonin receptor expressed in human retina and brain: the Mel1b melatonin receptor. Proc. Natl Acad. Sci. USA 92, 8734–8738.
| Molecular characterization of a second melatonin receptor expressed in human retina and brain: the Mel1b melatonin receptor.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXotVGqsrk%3D&md5=666e8dea947acdea388b1b2dd2322707CAS |
Rollag, M. D., and Niswender, G. D. (1976). Radioimmunoassay of serum concentrations of melatonin in sheep exposed to different lighting regimens. Endocrinology 98, 482–489.
| Radioimmunoassay of serum concentrations of melatonin in sheep exposed to different lighting regimens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE28XhtFyjur8%3D&md5=f624bd457d5ef5287ca7c28df10486bcCAS | 1248456PubMed |
Rousset, F. (2008). genepop’007: a complete reimplementation of the genepop software for Windows and Linux. Mol. Ecol. Resources 8, 103–106.
| genepop’007: a complete reimplementation of the genepop software for Windows and Linux.Crossref | GoogleScholarGoogle Scholar |
Salamon, S., and Maxwell, W. M. C. (2000). Storage of ram semen. Anim. Reprod. Sci. 62, 77–111.
| Storage of ram semen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXlt1Kgsbk%3D&md5=4e93a4798f185390e9b39bfb779e2a84CAS | 10924821PubMed |
Souza, C. J. H., Campbell, B. K., and Baird, D. T. (1997). Follicular dynamics and ovarian steroid secretion in sheep during the follicular and early luteal phases of the estrous cycle. Biol. Reprod. 56, 483–488.
| Follicular dynamics and ovarian steroid secretion in sheep during the follicular and early luteal phases of the estrous cycle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXos1ektQ%3D%3D&md5=903cd89985d978801028102d556d5811CAS | 9116150PubMed |
Sugino, N., Takiguchi, S., Kashida, S., Karube, A., Nakamura, Y., and Kato, H. (2000). Superoxide dismutase expression in the human corpus luteum during the menstrual cycle and in early pregnancy. Mol. Hum. Reprod. 6, 19–25.
| Superoxide dismutase expression in the human corpus luteum during the menstrual cycle and in early pregnancy.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXptFCltQ%3D%3D&md5=810bbce7809a3f9d34847648ed5af2c8CAS | 10611256PubMed |
Tamura, H., Takasaki, A., Miwa, I., Taniguchi, K., Maekawa, R., Asada, H., Taketani, T., Matsuoka, A., Yamagata, Y., Shimamura, K., Morioka, H., Ishikawa, H., Sugino, N., and Reiter, R. J. (2008). Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate. J. Pineal Res. 44, 280–287.
| Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXks1ansLk%3D&md5=39b43808c440c9955d7d0e139cf3b8a4CAS | 18339123PubMed |
Tamura, H., Nakamura, Y., Korkmaz, A., Manchester, L. C., Tan, D., Sugino, N., and Reiter, R. J. (2009). Melatonin and the ovary: physiological and pathophysiological implications. Fertil. Steril. 92, 328–343.
| Melatonin and the ovary: physiological and pathophysiological implications.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtFWisLrP&md5=aabd080f12c0d212112397a7a3096eebCAS | 18804205PubMed |
Teyssier, J., Migaud, M., Debus, N., Maton, C., Bouquet, P. M., Bocquier, F., Chemineau, P., Malpaux, B., and Bodin, L. (2006). Expression of seasonality in the Merinos d’Arles ewe according to the genotype of the melatonin receptor (MT1) gene and the feeding level. In ‘Proceeding of the 8th World Congress on Genetics Applied to Livestock Production, Belo Horizonte, 13–18 August 2006’. pp. 11–14. (WCGALP (Belo Horizonte, MG): Brazil.)
Weaver, D. R., Liu, C., and Reppert, S. M. (1996). Nature’s knock-out: the Mel1b receptor is not necessary for reproductive and circadian responses to melatonin in Siberian hamsters. Mol. Endocrinol. 10, 1478–1487.
| Nature’s knock-out: the Mel1b receptor is not necessary for reproductive and circadian responses to melatonin in Siberian hamsters.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XmvFShs7s%3D&md5=0c598a0726977739416037170f8697a1CAS | 8923472PubMed |
Yie, S. M., Niles, L. P., and Younglai, E. V. (1995). Melatonin receptors on human granulosa cell membranes. J. Clin. Endocrinol. Metab. 80, 1747–1749.
| Melatonin receptors on human granulosa cell membranes.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK2M3mtlOntw%3D%3D&md5=b8724e521833b0ce2c9baaea089b475eCAS | 7745030PubMed |
