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RESEARCH ARTICLE
Contents Vol 22(1)

Delivery of cloned offspring: experience in Zebu cattle (Bos indicus)

Flávio V. Meirelles A E , Eduardo H. Birgel Jr B , Felipe Perecin A , Marcelo Bertolini D , Anneliese S. Traldi B , José Rodrigo V. Pimentel B , Eliza R. Komninou B , Juliano R. Sangalli A , Paulo Fantinato Neto A , Mariana Tikuma Nunes B , Fábio Celidonio Pogliani B , Flávia D. P. Meirelles A , Flávia S. Kubrusly C , Camila I. Vannucchi B and Liege C. G. Silva B
+ Author Affiliations
- Author Affiliations

A Faculty of Animal Sciences and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225. Pirassununga, SP 13635-900, Brazil.

B Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, Av. Orlando Marques de Paiva, 87. Pirassununga, SP 05508-270, Brazil.

C Instituto Butantan, Av. Vital Brasil, 1500 São Paulo, SP 05503-900, Brazil.

D Center of Agronomy and Veterinary Sciences, Santa Catarina State University, Av. Luiz de Camões, 2090. Lages, SC 88520-000, Brazil.

E Corresponding author. Email: meirellf@usp.br

Reproduction, Fertility and Development 22(1) 88-97 https://doi.org/10.1071/RD09229
Published: 8 December 2009

Abstract

The production of a healthy cloned calf is dependent on a multitude of successful steps, including reprogramming mediated by the oocyte, the development of a functional placenta, adequate maternal–fetal interaction, the establishment of a physiological metabolic setting and the formation of a complete set of well-differentiated cells that will eventually result in well-characterised and fully competent tissues and organs. Although the efficiency of nuclear transfer has improved significantly since the first report of a somatic cell nuclear transfer-derived animal, there are many descriptions of anomalies concerning cloned calves leading to high perinatal morbidity and mortality. The present article discusses some our experience regarding perinatal and neonatal procedures for cloned Zebu cattle (B. indicus) that has led to improved survival rates in Nellore cloned calves following the application of such ‘labour-intensive technology’.

Additional keywords: bovine, nuclear transfer, parturition.


Acknowledgements

The authors thank Dr Lawrence C. Smith for his valuable comments on the text and Dr Isaias Raw from the Butantan Foundation for providing the surfactant used in our clone animals. Finally, the authors acknowledge the collaboration with Clonest Ltd and their clients, which provided the opportunity to deal with cloned animals.


References

Ardran, G. M. , Dawes, G. S. , and Prichard, M. M. (1952). The effect of ventilation of the fetal lungs upon the pulmonary circulation. J. Physiol. 118, 12–22.
| PubMed |  CAS | Herfen K., and Bostedt H. (1999). [Acid-base status in newborn calves during the first days of life considering different states of vitality.] Berl. Munch. Tierarztl. Wochenschr. 112, 166–171. [In German] Pubmed Abstract

Heyman, Y. (2005). Nuclear transfer: a new tool for reproductive biotechnology in cattle. Reprod. Nutr. Dev. 45, 353–361.
| Crossref | GoogleScholarGoogle Scholar | PubMed | Hill J. R., and Chavatte-Palmer P. (2002). Pregnancy and neonatal care of cloned animals. In ‘Principles of Cloning’. (Eds J. Cibelli, R. Lanza, K. Campbell and M. West.) pp. 247–266. (Academic Press: New York.)

Hill, J. R. , Roussel, A. J. , Cibelli, J. B. , Edwards, J. F. , and Hooper, N. L. , et al. (1999). Clinical and pathologic features of cloned transgenic calves and fetuses (13 case studies). Theriogenology 51, 1451–1465.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS | Sullivan H. E., Orsini J. A., and Divers T. J. (2008). Foal cardiopulmonary resuscitation. In ‘Manual of Equine Emergencies’. (Eds T. J. Divers and J. A. Orsini.) pp. 740–743. (Saunders: Philadelphia.)

Szenci, O. , Fazekas, Z. , and Tores, I. (1980). Treatment of asphyctic newborn calves with Dopram-V. Magyar Allatorvosok Lapja 35, 420–422.
House J. K. (2002). Postpartum assessment and care of the newborn ruminants. In ‘Large Animal Internal Medicine’. (Ed. B. P. Smith.) p. 279. (Mosby: St Louis.)

Wells, D. N. , Misica, P. M. , and Tervit, H. R. (1999). Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. Biol. Reprod. 60, 996–1005.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |

Wells, D. N. , Forsyth, J. T. , McMillan, V. , and Oback, B. (2004). The health of somatic cell cloned cattle and their offspring. Cloning Stem Cells 6, 101–110.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |

Wilmut, I. (2006). Are there any normal clones? Methods Mol. Biol. 348, 307–318.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |

Wilmut, I. , Schnieke, A. E. , McWhir, J. , Kind, A. J. , and Campbell, K. H. (1997). Viable offspring derived from fetal and adult mammalian cells. Nature 385, 810–813.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |

Wilmut, I. , Sullivan, G. , and Taylor, J. (2009). A decade of progress since the birth of Dolly. Reprod. Fertil. Dev. 21, 95–100.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |