Free Standard AU & NZ Shipping For All Book Orders Over $80!
Register      Login
Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
Shopping Cart: ( empty )
You are here: Home > Journals > RD > RD09226
RESEARCH ARTICLE
Contents Vol 22(1)

New approaches to superovulation in the cow

Gabriel A. Bó A E , Daniel Carballo Guerrero A B , Andrés Tríbulo A B , Humberto Tríbulo A B , Ricardo Tríbulo A B , Dragan Rogan C and Reuben J. Mapletoft D
+ Author Affiliations
- Author Affiliations

A Instituto de Reproducción Animal Córdoba (IRAC), Zona Rural General Paz, 5145 Córdoba, Argentina.

B Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, Avenida Valparaíso y Rogelio Martínez, Ciudad Universitaria, 5000 Córdoba, Argentina.

C Bioniche Life Sciences, 231 Dundas Street, East Belleville, ON K8N 5J2, Canada.

D Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada.

E Corresponding author. Email: gabrielbo@iracbiogen.com.ar

Reproduction, Fertility and Development 22(1) 106-112 https://doi.org/10.1071/RD09226
Published: 8 December 2009

Abstract

There is continuing need to simplify bovine superovulation protocols without compromising embryo production. The control of follicular wave emergence and ovulation has facilitated donor management, but the most commonly used treatment, oestradiol, cannot be used in many parts of the world and mechanical removal of the dominant follicle is difficult to apply in the field. Other alternatives include gonadotrophin-releasing hormone (GnRH) or LH, but efficacy in groups of randomly cycling animals is variable. Another alternative is to increase the response to GnRH by inducing a persistent follicle and initiating FSH treatments following GnRH-induced ovulation. The number of transferable embryos following superovulation during the first follicular wave did not differ from that achieved 4 days after oestradiol benzoate and progesterone. To further simplify superovulation, FSH has been administered as a single intramuscular injection. Superovulation of beef donors with a single intramuscular injection of Folltropin-V (Bioniche Animal Health, Belleville, ON, Canada) diluted in a slow-release formulation resulted in embryo production comparable to that obtained using the traditional twice-daily protocol. The single intramuscular injection has the potential to reduce labour and handling and may be useful when handling stress is an impediment to success. These alternatives provide ways of facilitating widespread application of embryo transfer technologies.

Additional keywords: first follicular wave, FSH, GnRH, progesterone releasing device, slow release formulation.


Acknowledgements

The authors’ research reported herein was supported by the Instituto de Reproducción Animal Córdoba (IRAC) and Bioniche Animal Health (Canada). The authors thank Biotay SA (Argentina) for the provision on PGF2α and GnRH and for facilitating the importation of the other hormones used in these trials. The authors extend special thanks to colleagues at IRAC for technical assistance.


References

Adams, G. P. , Nasser, L. F. , Bó, G. A. , Garcia, A. , Del Campo, M. R. , and Mapletoft, R. J. (1994). Superovulatory response of ovarian follicles of wave 1 versus wave 2 in heifers. Theriogenology 42, 1103–1113.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS | Callejas S. S., Alberio R., Cabodevila J. A., Dulout F., Aller J., and Teruel M. (2002). Ovarian stimulation with FSH-P in single dose in polyvinylpirrolidone or the combination of a reduced dose of FSH-P and eCG. Rev. Arg. Prod. Anim. 22, 141–151. [In Spanish with English Abstract]

Carballo Guerrero D., Tríbulo A., Tríbulo R., Tríbulo H., and Bó G. A. (2008a). Superovulation in the first follicular wave, without the use of estradiol in bonsmara cattle. Reprod. Fertil. Dev. 20, 226. [Abstract] doi:10.1071/RDV20N1AB294

Carballo Guerrero D., Tríbulo A., Tríbulo R., Tríbulo H., Mapletoft R. J., and Bó G. A. (2008b). Efeito do protocolo de pré-sincronização na resposta superovulatória na primeira onda folicular em doadoras brangus. Acta Scientiae Veterinariae 36(Suppl. 2), 631. [Abstract]

Carballo Guerrero D., Tríbulo A., Tríbulo R., Tríbulo H., and Bó G. A. (2009). Superovulatory response in beef cattle treated during the first follicular wave following synchronization of ovulation with a progestin device and GnRH. Reprod. Fertil. Dev. 21, 242–243. [Abstract] doi:10.1071/RDV21N1AB291

Carballo Guerrero D., Tríbulo A., Tríbulo R., Tríbulo H., and Bó G. A. (2010). Superovulatory response in beef donors treated during the first follicular wave or four days after progesterone and estradiol administration. Reprod. Fertil. Dev. 22, 358. [Abstract] doi:10.1071/RDV22N1AB404

Choi, S. H. , Park, Y. S. , Cho, S. R. , Kang, T. Y. , Sin, S. H. , Kang, S. S. , Rho, G. J. , and Choe, S. Y. (2002). Superovulation response and quality of embryos recovered from cattle after a single subcutaneous injection of FSH dissolved in polyethylene glycol. Korean J. Embryo Trans. 17, 67–77.
Deyo C. D., Colazo M. G., Martinez M. F., and Mapletoft R. J. (2001). The use of GnRH or LH to synchronize follicular wave emergence for superovulation in cattle. Theriogenology 55, 513. [Abstract]

Dieleman, S. J. , Bevers, M. M. , Vos, P. L. A. M. , and de Loos, F. A. M. (1993). PMSG/anti-PMSG in cattle: a simple and efficient superovulatory treatment? Theriogenology 39, 25–41.
| Crossref | GoogleScholarGoogle Scholar | Rogan D., Tríbulo A., Tríbulo H., Tríbulo R., Carballo Guerrero D., Tríbulo P., Mapletoft R. J., and Bó G. A. (2010). Dose titration for superstimulation of Brangus Bonsmara donors with Folltropin®-V by a single intramuscular injection. Reprod. Fertil. Dev. 22, 365. [Abstract] doi:10.1071/RDV22N1AB416

Schams D., Menzer D., Schalenberger E., Hoffman B., Hahn J., and Hahn R. (1977). Some studies of the pregnant mare serum gonadotrophin (PMSG) and on endocrine responses after application for superovulation in cattle. In ‘Control of Reproduction in the Cow’. (Ed. J. M. Sreenan.) pp. 122–142. (Martinus Nijhoff: The Hague.)

Small, J. A. , Colazo, M. G. , Kastelic, J. P. , and Mapletoft, R. J. (2009). Effects of progesterone presynchronization and eCG on pregnancy rates to GnRH-based, timed-AI in beef cattle. Theriogenology 71, 698–706.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS | Steel R. G., and Hasler J. F. (2009). Comparison of three different protocols for superstimulation of dairy cattle. Reprod. Fertil. Dev. 21, 246. [Abstract] doi:10.1071/RDV21N1AB298

Stoebel, D. P. , and Moberg, G. P. (1982). Repeated acute stress during the follicular phase and luteinizing hormone surge of dairy heifers. J. Dairy Sci. 65, 92–96.
| PubMed |  CAS | Tríbulo A., Tríbulo H., Tríbulo R., Carballo Guerrero D., Tríbulo P., Rogan D., Mapletoft R. J., and Bó G. A. (2010). Superstimulation of angus donors with a single intramuscular injection of Folltropin®-V. Reprod. Fertil. Dev. 22, 367. [Abstract] doi:10.1071/RDV22N1AB420

Walsh, J. H. , Mantovani, R. , Duby, R. T. , Overstrom, E. W. , Dobrinsky, J. R. , Enright, W. J. , Roche, J. F. , and Boland, M. P. (1993). The effects of once or twice daily injections of p-FSH on superovulatory response in heifers. Theriogenology 40, 313–321.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS | Wock J. M., Lyle L. M., and Hockett M. E. (2008). Effect of gonadotropin-releasing hormone compared with estradiol-17β at the beginning of a superovulation protocol on superovulatory response and embryo quality. Reprod. Fertil. Dev. 20, 228. [Abstract] doi:10.1071/RDV20N1AB297

Yamamoto, M. , Oow, M. , Kawaguchi, M. , and Suzuki, T. (1994). Superovulation in the cow with a single intramuscular injection of FSH disolved in polyvinylpyrrolidone. Theriogenology 41, 747–755.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |