Effect of human β-globin bacterial artificial chromosome transgenesis on embryo cryopreservation in mouse models
Duangjai Boonkusol A I , Andras Dinnyes B , Tassanee Faisaikarm C , Parisatcha Sangsuwan C , Nathnapith Pratipnatalang D , Mayurachat Sa-ardrit D , Kulnasan Saikhun C , Saovaros Svasti E F , Jim Vadolas G , Pranee Winichagoon E , Suthat Fucharoen E and Yindee Kitiyanant C HA Department of Biology, Faculty of Science, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand.
B Molecular Animal Biotechnology Laboratory, Szent Istvan University, 2100, Godollo, Hungary.
C Research Centre on Reproductive Biology of Economically Valuable Animals, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakornpathom 73170, Thailand.
D National Laboratory Animal Centre, Mahidol University, Salaya, Nakornpathom 73170, Thailand.
E Thalassaemia Research Centre, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakornpathom 73170, Thailand.
F Department of Biochemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
G Cell and Gene Therapy Research Group, The Murdoch Childrens Research Institute, The University of Melbourne, Royal Children’s Hospital, Flemington Road, Parkville, Vic. 3052, Australia.
H Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
I Corresponding author. Email: ngamsomd@yahoo.com
Reproduction, Fertility and Development 22(5) 788-795 https://doi.org/10.1071/RD09128
Submitted: 28 May 2009 Accepted: 28 October 2009 Published: 7 April 2010
Abstract
The purpose of the present study was to investigate the efficiency of embryo cryopreservation for four transgenic (TG) thalassaemic mouse strains, which is a key element of the ongoing gene banking efforts for these high-value animals. Heterozygous TG embryos were produced by breeding four lines of TG males to wild-type (WT) females (C57BL/6J). Intact two-cell embryos were cryopreserved by vitrification in straws using 35% ethylene glycol. Survival rates of cryopreserved embryos ranged between 91.1% (102/112) and 93.6% (176/188) without significant differences between the lines. In contrast, the paternal line had a significant effect on the development of these embryos to the blastocyst stage, which ranged from 50.6% (92/182) to 77.5% (79/102). This effect was also noted following embryo transfers, with implantation rates varying from 17.3% (19/110) to 78.1% (35/45). The results demonstrate that the in vivo developmental potential is significantly influenced by TG line and reveal a specific line effect on cryosurvival. All bacterial artificial chromosome transgenic fetuses developed from vitrified–warmed embryos showed expression of the human β-globin transgene. In conclusion, the present study shows a strong TG line effect on developmental competence following cryopreservation and the vitrification method was successful to bank the human β-globin TG-expressing mouse strains.
Additional keywords: embryo banking, thalassaemia transgenic.
Acknowledgements
This work was supported by grants from The Thailand Research Fund and Commission on Higher Education (grant no. MRG5080212), and Mahidol University (grant no. 02011868–0004). The authors thank Professor Duncan Richard Smith for critical reading and help with the preparation of the manuscript, Mrs Kanchana Kengkoom, National Laboratory Animal Centre, for providing facilities support and Ms Thongperm Munkongdee for assistance with data analysis.
Boonkusol, D. , Gal, A. B. , Bodo, S. , Gorhony, B. , Kitiyanant, Y. , and Dinnyes, A. (2006). Gene expression profiles and in vitro development following vitrification of pronuclear and 8-cell stage mouse embryos. Mol. Reprod. Dev. 73, 700–708.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |
Byers, S. L. , Payson, S. J. , and Taft, R. A. (2006). Performance of ten inbred mouse strains following assisted reproductive technologies (ARTs). Theriogenology 65, 1716–1726.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Carpino, A. , Tarantino, P. , Rago, V. , De Sanctis, V. , and Siciliano, L. (2004). Antioxidant capacity in seminal plasma of transfusion-dependent β-thalassaemic patients. Exp. Clin. Endocrinol. Diabetes 112, 131–134.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |
Dinnyés, A. , Wallace, G. A. , and Rall, W. F. (1995). Effect of genotype on the efficiency of mouse embryo cryopreservation by vitrification or slow freezing method. Mol. Reprod. Dev. 40, 429–435.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Dinnyés, A. , Dai, Y. , Jiang, S. , and Yang, X. (2000). High development rates of vitrified bovine oocytes following parthenogenetic activation, in vitro fertilization, and somatic nuclear transfer. Biol. Reprod. 63, 513–518.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Fahy, G. M. (1983). Cryoprotectant toxicity neutralizers reduce freezing injury. Cryo Letters 4, 309–314.
| CAS |
Ibañez, E. , Grossmann, M. , Vidal, F. , Egozcue, J. , and Santalo, J. (1997). Cryopreservation of caprine beta-lactoglobulin transgenic mouse embryos. Cryobiology 35, 290–298.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Jamsai, D. , Zaibak, F. , Vadolas, J. , Voullaire, L. , Fowler, K. J. , Gazeas, S. , Peters, H. , Fucharoen, S. , Williamson, R. , and Ioannou, P. A. (2006). A humanized mouse model for a common β0-thalassaemia mutation. Genomics 88, 309–315.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |
Kasai, M. , Komi, J. H. , Takakamo, A. , Tsudera, H. , Sakurai, T. , and Machida, T. (1990). A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J. Reprod. Fertil. 89, 91–97.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |
Leibo, S. P. , Mazur, P. , and Jackowski, S. J. (1974). Factors affecting survival of mouse embryos during freezing and thawing. Exp. Cell Res. 89, 79–88.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |
Lewis, J. , Yang, B. , Kim, R. , Sierakowska, H. , Kole, R. , Smithies, O. , and Maeda, N. (1998). A common human β globin splicing mutation modeled in mice. Blood 91, 2152–2156.
| PubMed | CAS |
Luyet, B. J. (1969). On the amount of liquid water remaining amorphous in frozen aqueous solutions. Biodynamica 10, 277–291.
Luyet, B. J. , and Rasmussen, D. H. (1968). Study by differential thermal analysis of the temperatures of instability of rapidly cooled solutions of glycerol, ethylene glycol, sucrose and glucose. Biodynamica 10, 167–191.
Mamo, S. , Bodo, S. , Kobolak, J. , Polgar, Z. , Tolgyesi, G. , and Dinnyes, A. (2006). Gene expression profiles of vitrified in vivo derived 8-cell stage mouse embryos detected by high density oligonucleotide microarrays. Mol. Reprod. Dev. 73, 1380–1392.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |
Palasz, A. T. , and Mapletoft, R. J. (1996). Cryopreservation of mammalian embryos: recent advances. Biotechnol. Adv. 14, 127–149.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |
Perera, D. , Pizzey, A. , Campbell, A. , Katz, M. , Porter, J. , Petrou, M. , Irvine, D. S. , and Chatterjee, R. (2002). Sperm DNA damage in potentially fertile homozygous β-thalassaemia patients with iron overload. Hum. Reprod. 17, 1820–1825.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |
Radice, G. , Lee, J. L. , and Costantini, F. (1991). Hβ58, an insertional mutation affecting early postimplantation development of the mouse embryo. Development 111, 801–811.
| PubMed | CAS |
Rall, W. F. , and Fahy, G. M. (1985). Ice-free cryopreservation of mouse embryos at –196°C by vitrification. Nature 313, 573–575.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |
Rall, W. F. , Schmidt, P. M. , Lin, X. , Brown, S. S. , Ward, A. C. , and Hansen, C. T. (2000). Factors affecting the efficiency of embryo cryopreservation and rederivation of rat and mouse models. ILAR J. 41, 221–227.
| PubMed | CAS |
Schmidt, P. M. , Hansen, C. T. , and Wildt, D. E. (1985). Viability of frozen–thawed mouse embryos is affected by genotype. Biol. Reprod. 32, 507–514.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |
Schmidt, P. M. , Schiewe, M. C. , and Wildt, D. E. (1987). The genotypic respose of mouse embryos to multiple freezing variables. Biol. Reprod. 37, 1121–1128.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |
Whitelaw, E. , Tsai, S. F. , Hogben, P. , and Orkin, S. H. (1990). Regulated expression of globin chains and the erythroid transcription factor GATA-1 during erythropoiesis in the developing mouse. Mol. Cell. Biol. 10, 6596–6606.
| PubMed | CAS |
Whittingham, D. G. , Leibo, S. P. , and Mazur, P. (1972). Survival of mouse embryos frozen to –196°C and –269°C. Science 178, 411–414.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |
Wilmut, I. (1972). The effect of cooling rate, warming rate, cryoprotective agent and stage of development on survival of mouse embryos during freezing and thawing. Life Sci. 11, 1071–1079.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |
Yang, B. , Kirby, S. , Lewis, J. , Detloff, P. J. , Maeda, N. , and Smithies, O. (1995). A mouse model for β0-thalassaemia. Proc. Natl. Acad. Sci. USA 92, 11 608–11 612.
| Crossref | GoogleScholarGoogle Scholar | PubMed | CAS |
