Effect of the conceptus on uterine prostaglandin-F2α and prostaglandin-E2 release and synthesis during the periimplantation period in the pig
Marta Wasielak A , Katarzyna Kamińska A and Marek Bogacki A B
+ Author Affiliations
- Author Affiliations
A Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland.
B Corresponding author. Email: marbo@pan.olsztyn.pl
Reproduction, Fertility and Development 21(5) 709-717 https://doi.org/10.1071/RD08236
Submitted: 20 October 2008 Accepted: 8 March 2009 Published: 22 May 2009
Abstract
The present study was conducted to evaluate the effect of the conceptus on uterine prostaglandin-F2α (PGF2α) and prostaglandin-E2 (PGE2) release and the expression of prostaglandin synthase enzymes during the periimplantation period in the pig. A surgically generated model with conceptuses developing in only one of the uterine horns was created. The highest concentration of PGF2α and PGE2 was found in the gravid uterine horn, compared with the non-gravid horn and the intact horn of cyclic gilts. Endometrial concentration of both PGs in pregnant gilts was elevated regardless of the conceptus in the uterine horn, whereas only myometrial PGE2 concentration increased during pregnancy. Expression of prostaglandin-E2 synthase (mPGES-1) mRNA in the endometrium was upregulated during the oestrous cycle, while protein expression presented a similar pattern to that of PGE2 concentration in the uterine flushings. Prostaglandin-F2α synthase (PGFS) mRNA and protein expression in the endometrium did not differ between pregnancy and oestrous cycle but PGFS mRNA in the myometrium increased during pregnancy both in the gravid and the non-gravid uterine horns. We suggest a local effect of the conceptus on PG release pathways but also a more systemic effect within the whole uterus with regard to PG synthesis and accumulation in uterine tissues.
Additional keywords: early pregnancy, prostaglandins, uterus.
Acknowledgements
This research was supported by Grant 2 P06D 026 29 from the State Committee for Scientific Research in Poland.
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