Effect of type of cryoprotectant on morphology and developmental competence of in vitro-matured buffalo (Bubalus bubalis) oocytes subjected to slow freezing or vitrification
S. K. Gautam A , V. Verma A , P. Palta A , M. S. Chauhan A and R. S. Manik A B
+ Author Affiliations
- Author Affiliations
A Animal Biotechnology Centre, National Dairy Research Institute, Karnal 132 001, India.
B Corresponding author. Email: manik_rs@rediffmail.com
Reproduction, Fertility and Development 20(4) 490-496 https://doi.org/10.1071/RD07203
Submitted: 7 November 2007 Accepted: 4 February 2008 Published: 11 April 2008
Abstract
The present study examined the effects of different cryoprotectants on morphology and developmental competence of in vitro-matured buffalo oocytes after slow freezing or vitrification. After slow freezing in dimethyl sulfoxide (DMSO), ethylene glycol (EG) or 1,2-propanediol (PROH), at 1.0 or 1.5 m each, the proportion of morphologically normal oocytes recovered was significantly higher (P < 0.05) with 1.5 than 1.0 m for all cryoprotectants and was highest (P < 0.05) for 1.5 m DMSO. Following vitrification, the percentage of morphologically normal oocytes recovered was lower (P < 0.01) for 40% EG than for 40% DMSO, 20% EG + 20% DMSO or 20% EG + 20% PROH. The most common damage, irrespective of the cryopreservation method, was loss of cumulus mass. The cleavage rate and the proportion of vitrified–warmed oocytes that developed to morulae/blastocysts were significantly higher (P < 0.01) for 20% EG + 20% DMSO than for the other groups. A higher proportion of oocytes developed to morulae (11.5% v. 4.3%) or blastocysts (5.4% v. 0.6%) after vitrification in 20% EG + 20% DMSO than after slow freezing in 1.5 m DMSO. In conclusion, vitrification was more effective than slow freezing for the cryopreservation of in vitro-matured buffalo oocytes.
References
Aigner, S. , Van der Elst, J. , Siebzehnrubl, E. , Wildt, L. , Lang, N. , and Van Steirteghem, A. C. (1992). The influence of slow and ultra-rapid freezing on the organization of the meiotic spindle of the mouse oocytes. Hum. Reprod. 7, 857–864.
| PubMed |
Almeida, P. A. , and Bolton, V. N. (1995). The effect of temperature fluctuations on the cytoskeletal organization and chromosomal constitution of the human oocyte. Zygote 3, 357–365.
| PubMed |
Albarracin, J. L. , Morato, R. , Rojas, C. , and Mogas, T. (2005). Effect of vitrification in open pulled straws on the cytology of in vitro matured prepubertal and adult bovine oocytes. Theriogenology 63, 890–901.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Aman, R. R. , and Parks, J. E. (1994). Effects of cooling and rewarming on the meiotic spindle and chromosomes of in vitro matured bovine oocytes. Biol. Reprod. 50, 103–110.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Asada, M. , and Fukui, Y. (2000). Effect on fertilization and development by re-culture after freezing and thawing of bovine oocytes matured in vitro. Theriogenology 54, 889–898.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Chauhan, M. S. , Singla, S. K. , Palta, P. , Manik, R. S. , and Madan, M. L. (1998). In vitro maturation and fertilization, and subsequent development of buffalo (Bubalus bubalis) embryos: effects of oocyte quality and type of serum. Reprod. Fertil. Dev. 10, 173–177.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Dhali, A. , Manik, R. S. , Singla, S. K. , and Palta, P. (2000). Post-vitrification survival and in vitro maturation rate of buffalo (Bubalus bubalis) oocytes: effect of ethylene glycol concentration and exposure time. Anim. Reprod. Sci. 63, 159–165.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Fuku, E. , Kozima, T. , Shioya, Y. , Mercus, G. J. , and Downey, B. R. (1992). In vitro fertilization and development of frozen–thawed bovine oocytes. Cryobiology 29, 485–492.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Fuku, E. , Xia, L. , and Downey, B. R. (1995). Ultrastructural changes in bovine oocytes cryopreserved by vitrification. Cryobiology 32, 139–156.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Gasparrini, B. (2002). In vitro embryo production in buffalo species: state-of-the-art. Theriogenology 57, 237–256.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Gasparrini, B. , Attanasio, L. , De Rosa, A. , Monaco, E. , Di Palo, R. , and Campanile, G. (2007). Cryopreservation of in vitro matured buffalo (Bubalus bubalis) oocytes by minimum volumes vitrification methods. Anim. Reprod. Sci. 98, 335–342.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Gautam, S. K. , Verma, V. , Singh, B. , Palta, P. , Singla, S. K. , Chauhan, M. S. , and Manik, R. S. (2008). Effect of slow freezing on morphology and developmental competence of buffalo (Bubalus bubalis) immature oocytes. Anim. Reprod. Sci. 105, 311–318.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Gupta, V. , Manik, R. S. , Chauhan, M. S. , Singla, S. K. , Akshey, Y. S. , and Palta, P. (2006). Repeated ultrasound-guided transvaginal oocyte retrieval from cyclic Murrah buffaloes (Bubalus bubalis): oocyte recovery and quality. Anim. Reprod. Sci. 91, 89–96.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Im, K. S. , Kang, J. K. , and Kim, H. S. (1997). Effects of cumulus cells, different cryoprotectants, various maturation stages and preincubation before insemination on developmental capacity of frozen–thawed bovine oocytes. Theriogenology 47, 881–891.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Kumar, D. , Palta, P. , Manik, R. S. , Singla, S. K. , and Chauhan, M. S. (2007). Effect of culture media and serum supplementation on the development of in vitro fertilized buffalo embryos. Indian J. Anim. Sci. 77, 697–701.
Le Gal, F. , De Roover, R. , Verhaeghe, B. , Etienne, D. , and Massip, A. (2000). Development of vitrified matured cattle oocytes after thawing and culture in vitro. Vet. Rec. 146, 469–471.
| PubMed |
Lim, J. M. , Ko, J. J. , Hwang, H. M. , and Niwa, K. (1999). Development of in vitro matured bovine oocytes after cryopreservation with different cryoprotectants. Theriogenology 51, 1303–1310.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Martino, A. , Songasen, N. , and Leibo, S. P. (1996). Development into blastocysts of bovine oocytes cryopreserved by ultra-rapid cooling. Biol. Reprod. 54, 1059–1069.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Mavrides, A. , and Morroll, D. (2002). Cryopreservation of bovine oocytes: is cryoloop vitrification the future to preserving the female gamete? Reprod. Nutr. Dev. 42, 73–80.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Otoi, T. , Tachikawa, S. , Kondo, S. , and Suzuki, T. (1993). Developmental capacity of bovine oocytes frozen in different cryoprotectants. Theriogenology 40, 801–807.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Otoi, T. , Tachikawa, S. , Kondo, S. , and Suzuki, T. (1994). Developmental competence of bovine oocytes frozen at different cooling rates. Cryobiology 31, 344–348.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Otoi, T. , Yamamoto, K. , Koyama, N. , and Suzuki, T. (1995). In vitro fertilization and development of immature and mature bovine oocytes cryopreserved by ethylene glycol with sucrose. Cryobiology 32, 455–460.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Otoi, T. , Yamamoto, K. , Koyama, N. , Tachikawa, S. , and Suzuki, T. (1998). Cryopreservation of mature bovine oocytes by vitrification in straws. Cryobiology 37, 77–85.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Palta, P. , and Chauhan, M. S. (1998). Laboratory production of buffalo (Bubalus bubalis) embryos. Reprod. Fertil. Dev. 10, 379–391.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Saunders, K. M. , and Parks, J. E. (1999). Effects of cryopreservation procedures on the cytology and fertilization rate of in vitro matured bovine oocytes. Biol. Reprod. 61, 178–187.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Schellander, K. , Peli, S. , Schmoli, F. , and Brem, G. (1994). Effects of different cryoprotectants and carbohydrates on freezing of mature and immature bovine oocytes. Theriogenology 42, 909–915.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Vajta, G. , Holm, P. , Kuwayama, M. , Booth, P. J. , Jacobsen, H. , Greve, T. , and Callesen, H. (1998). Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine oocytes and embryos. Mol. Reprod. Dev. 51, 53–58.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Wani, N. A. , Misra, A. K. , and Maurya, S. N. (2004). Effect of cryoprotectants and their concentration on in vitro development of vitrified–warmed immature oocytes in buffalo (Bubalus bubalis). Theriogenology 61, 831–842.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Zenzes, M. T. , Bielecki, R. , Casper, R. F. , and Leibo, S. P. (2001). Effects of chilling to 0°C on the morphology of meiotic spindles in human metaphase II oocytes. Fertil. Steril. 75, 769–777.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
