Uterine expression of implantation serine proteinase 2 during the implantation period and in vivo inhibitory effect of its antibody on embryo implantation in mice
Z. P. Huang A B , H. Yu C , Z. M. Yang C , W. X. Shen A , J. Wang A B D and Q. X. Shen AA State Family Planning Commission Key Laboratory of Contraceptive Drugs and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China.
B Shanghai Medical School, Fudan University, Shanghai 200032, China.
C College of Life Science, Northeast Agriculture University, Harbin 150030, China.
D To whom correspondence should be addressed. email: kjb@sippr.stc.sh.cn
Reproduction, Fertility and Development 16(3) 379-384 https://doi.org/10.1071/RD03102
Submitted: 6 November 2003 Accepted: 7 April 2004 Published: 25 May 2004
Abstract
The aim of the present study was to examine the uterine expression pattern of implantation serine proteinase 2 (ISP2) protein during early pregnancy in mice and the effects of anti-ISP2 antibody on embryo implantation. Expression of ISP2 protein was found to be specifically up-regulated in mouse uterine endometrial glands following the initiation of embryo implantation. Similarly, ISP2 protein expression was observed during pseudopregnancy, indicating that its expression is not embryo dependent. In other experiments, rabbit anti-ISP2 IgG was infused into the mouse uterine lumen on Day 3 or 4 of pregnancy to examine its effects on embryo implantation, whereas vehicle (saline) or unspecific rabbit IgG served as controls. The mean number of implanted embryos from anti-ISP2-IgG-treated mice was significantly lower than that from control mice. These results suggest that ISP2 may play an important role during embryo implantation.
Extra keywords: ISP2, uterus.
Acknowledgments
This study was supported by the National Basic Research Programme (Project No. TG1999055903) and the National Natural Science Foundation of China (Project No. 30300374).
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