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Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
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Reproduction, Fertility and Development

Reproduction, Fertility and Development

Volume 36 Number 17 2024

RD24125Ovarian follicular flushing as a means of increasing oocyte yield and in vitro embryo production in cattle

R. J. Simmons 0000-0002-2422-6480, D. A. R. Tutt, W. Y. Kwong, J. I. Baroni, L. N. Lim, R. Cimpeanu, A. A. Castrejon-Pita, M. Vatish, P. Svensson, R. Piegsa, U. Hagby, K. D. Sinclair 0000-0002-6375-215X and E. X. Georgiou

Image of the novel 16G double-lumen needle (‘OxIVF’), showing how it flushes laterally to the needle shaft.

The number of oocytes retrieved during assisted reproduction is a key determinant of success in both cattle and humans. Ovarian follicular flushing is not practised in cattle but offers the prospect of increasing oocyte yield and number of transferrable embryos. We determined that a ‘hybrid’ approach of flushing larger follicles and aspirating smaller follicles has potential in this regard. These findings serve as an impetus for follow-up studies in human and equine assisted reproduction. Image provided by authors.

This article belongs to the collection: The biology of the ovary – Honouring the contributions of Ken P McNatty and Rex J Scaramuzzi.

Committee on Publication Ethics

Announcement

Reproduction, Fertility and Development is now publishing technical reports, i.e. significant technical advances. More

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