Reproduction, Fertility and Development
Volume 32 Numbers 1 & 2 2020
Proceedings of the Annual Conference of the International Embryo Technology Society, New York, New York, 16–19 January 2020
Full Papers and Abstracts for Poster Presentation
RDv32n2refsIETS 2020 Section Editors and Manuscript and Abstract Reviewers
pp. i-i
RD19270In vitro growth of immature bovine follicles and oocytes
Evelyn E. Telfer, Kenichiro Sakaguchi, Yvonne L. Clarkson and Marie McLaughlin
pp. 1-6
RD19271Prospects for new oocyte-based assisted reproduction in animals and humans
David F. Albertini
pp. 7-10
RD19272Livestock pluripotency is finally captured in vitro
Micaela Navarro, Delia A. Soto, Carlos A. Pinzon, Jun Wu and Pablo J. Ross
pp. 11-39
RD19273Application of genome-editing systems to enhance available pig resources for agriculture and biomedicine
Kiho Lee, Kayla Farrell and Kyungjun Uh
pp. 40-49
RD19275Genetic and genomic analyses of embryo production in dairy cattle
C. Jaton, F. S. Schenkel, T. C. S. Chud, F. Malchiodi, M. Sargolzaei, C. A. Price, A. Canovàs, C. Baes and F. Miglior
pp. 50-55
RD19389Role of extracellular vesicles during oocyte maturation and early embryo development
A. C. F. C. M. de Ávila and J. C. da Silveira
pp. 56-64
RD19276Consequences of assisted reproductive techniques on the embryonic epigenome in cattle
Rocío Melissa Rivera
pp. 65-81
RD19276 Abstract | RD19276 Full Text | RD19276PDF (371 KB) Open Access Article
RD19278Consequences of assisted reproductive technologies for offspring function in cattle
Luiz G. Siqueira, Marcos V. G. Silva, João C. Panetto and João H. Viana
pp. 82-97
RD19255Bovine oocyte maturation: acquisition of developmental competence
Bernard A. J. Roelen 
pp. 98-103
RD19279Factors affecting embryo production in superovulated Bos taurus cattle
M. Mikkola, J. F. Hasler and J. Taponen
pp. 104-124
RDv32n2Ab11 Assessing the energy status of porcine embryos by means of biodynamic imaging
I. Lorenzo, Z. Li, M. Torres, Z. Machaty and D. Nolte
pp. 125-125
RDv32n2Ab22 The landscape of accessible chromatin in bovine oocytes and early embryos
H. Ming, J. Sun, R. Pasquariello, J. R. Herrick, Y. Yuan, E. Gutierrez, L. Gatenby, K. R. Bondioli, R. L. Krisher and Z. Jiang
pp. 125-125
RDv32n2Ab33 Dietary caloric normalization or restriction as preconception care strategies: Impact on metabolic health and fertility in high fat-induced obese outbred mice
A. Smits, W. Marei, M. De Ketelaere, B. Meulders, P. Bols and J. Leroy
pp. 126-126
RDv32n2Ab44 Sperm, but not seminal plasma, elicit changes in the bovine endometrial transcriptome after natural mating
S. Recuero, J. M. Sánchez, Y. Mateo-Otero, S. Bagés-Arnal, M. McDonald, S. K. Behura, T. E. Spencer, M. Yeste, P. Lonergan and B. Fernandez-Fuertes
pp. 126-126
RDv32n2Ab55 Transport and cholinergic innervation in the bovine oviduct are dysregulated in cystic ovary disease
D. Scully, D. Campion, F. McCartney, S. Reese and S. Kölle
pp. 127-127
RDv32n2Ab66 In vitro validation of gene edited phenotypes using CRISPR-dCas9 transcriptional activators
K. M. Polkoff, N. K. Gupta and J. A. Piedrahita
pp. 127-127
RDv32n2Ab77 Pregnancy from a vitrified-warmed alpaca pre-implantation embryo
J. Lutz, S. Johnson, K. Duprey, P. Taylor, H. Vivanco, M. Ponce-Salazar, M. Miguel and C. Youngs
pp. 128-128
RDv32n2Ab88 Relationship between oestrus manifestation and pregnancy rates on recipients used in an in vitro-produced embryo transfer program
B. M. Pasqual, E. E. Correa, E. P. da Silva, M. K. Sermersheim, L. M. Naves, S. G. Sell, B. R. Lindsey, M. Rubessa and M. B. Wheeler
pp. 128-129
RDv32n2Ab99 Pregnancy loss in Holstein lactating recipient cows diagnosed pregnant by pregnancy-associated glycoprotein test in blood
R. Santos, M. Oliveira, N. Melgar, R. Chebel and D. Demetrio
pp. 129-129
RDv32n2Ab1010 Pregnancy rates following artificial insemination or embryo transfer in lactating Holstein cows
M. Oliveira, R. Santos, R. Chebel and D. Demetrio
pp. 130-130
RDv32n2Ab1111 In vivo-derived embryo pregnancy rates at Maddox Dairy from 2008 to 2018
D. Demetrio, A. Magalhaes, M. Oliveira, R. Santos and R. Chebel
pp. 130-131
RDv32n2Ab1212 Assessment of oocyte and embryo production in senescent beef cows
R. H. Alvarez, B. M. Bayeux, Y. F. Watanabe, D. A. Joaquim, K. M. R. Duarte and G. Pugliesi
pp. 131-131
RDv32n2Ab1313 In vitro and in vivo embryo production during foal heat in a mare: A case report
L. F. Campos-Chillon and J. L. Altermatt
pp. 132-132
RDv32n2Ab1414 Effects of long cooling periods of the ear skin on the isolation and cultivation of bovine fibroblasts for posterior use in cloning via nuclear transfer
J. M. Araujo, R. A. Oliveira, H. C. B. Cumpa, A. T. M. Cunha, M. A. N. Dode and C. F. Martins
pp. 132-133
RDv32n2Ab1515 Combination of RepSox with histone deacetylation inhibitors on in vitro development competence of porcine somatic cell nuclear transfer embryos
Z.-B. Luo, M.-F. Xuan, Z.-Y. Li, X.-J. Yin and J.-D. Kang
pp. 133-133
RDv32n2Ab1616 Improvement of porcine cloned embryo developmental competence via KDM4A overexpression and H3K9me3 methyltransferase inhibitor treatment
Y.-T. Zhang, Y. Liu and Z. Liu
pp. 133-133
RDv32n2Ab1717 Generation of myostatin gene knockout boars by somatic cell nuclear transfer
J.-D. Kang, M.-F. Xuan, Z.-B. Luo, S.-Z. Han and X.-J. Yin
pp. 134-134
RDv32n2Ab1919 Improvement of the developmental competence of bovine somatic cell nuclear transfer embryos using latrunculin A during activation
G. Vans Landschoot, V. Savy, L. D. Ratner, V. Alberio and D. F. Salamone
pp. 135-135
RDv32n2Ab2020 Aggregation of yak heterospecific somatic cell nuclear transfer embryos improves cloning efficiency
M. Yauri Felipe, M. Duque Rodríguez, A. De Stéfano and D. Salamone
pp. 135-136
RDv32n2Ab2121 The contrasting role of histone methyltransferases during nuclear reprogramming: SUV39H knockdown improves bovine somatic cell nuclear transfer, while the absence of EHMT2 hampers it
R. Sampaio, D. Ambrizi, R. Nociti, J. Pinzon, J. Sangalli, T. De Bem, K. Takahashi, J. Cruz, L. Smith, P. Ross and F. Meirelles
pp. 136-136
RDv32n2Ab2222 The zona pellucida is required for normal development of in vitro-produced cat embryos
D. Veraguas, S. Saez, M. Cordero, C. Aguilera, D. Saez-Ruiz, F. O. Castro and L. Rodriguez-Alvarez
pp. 137-137
RDv32n2Ab2323 Sperm quality of Pure Spanish stallions is affected by inbreeding coefficient and age
Y. Pirosanto, M. Valera, A. Molina, J. Dorado and S. Demyda-Peyrás
pp. 137-137
RDv32n2Ab2424 Asynchronic tetraploid complementation and embryo quality in domestic cat and Leopardus geoffroyi hybrid embryos
M. Duque Rodriguez, A. Gambini, C. Gutnisky, L. Ratner, S. Rulli, A. Sestelo, O. Briski, R. Fernandez Martin, P. D. Cetica and D. Salamone
pp. 138-138
RDv32n2Ab2525 A preliminary study of the effects of breed and nanowater as extender diluent on ram semen characteristics post-thawing
M. Murawski, J. Szymanowicz, T. Schwarz and P. Bartlewski
pp. 138-139
RDv32n2Ab2626 Baobab oil supplemented extender preserves post-thaw bull sperm quality parameters
Z. Raphalalani, F. Ramukhithi, R. Ndhlala, K. Nephawe and T. Nedambale
pp. 139-139
RDv32n2Ab2727 Vitrification at the germinal vesicle stage does not trigger apoptosis in porcine oocytes and early embryos
T. Somfai, H. T. Nguyen, M. T. Nguyen, T. Q. Dang-Nguyen, H. Kaneko, J. Noguchi and K. Kikuchi
pp. 139-139
RDv32n2Ab2828 Short equilibration improves vitrification of in vitro-produced bovine embryos using VitTrans device
I. Martínez-Rodero, T. García-Martínez, M. López-Béjar and T. Mogas
pp. 140-140
RDv32n2Ab2929 Time-lapse analysis of bovine embryos derived after in vitro fertilization from vitrified and fresh oocytes
D. A. Velez, H. Atashi, J. Dewulf, K. Smits and A. Van Soom
pp. 140-140
RDv32n2Ab3030 Supplementation of in vitro culture medium with linoleic acid albumin improves bovine embryo survivability in low-temperature storage at 4°C
S. K. Jung, T. Nishisouzu, O. Dochi and K. Imai
pp. 141-141
RDv32n2Ab3131 Effect of vitrification on global gene expression dynamics of bovine elongating embryos
Z. Jiang, E. Gutierrez, H. Ming, B. Foster, L. Gatenby, C. Mak, C. Pinto and K. Bondioli
pp. 141-141
RDv32n2Ab3232 Bovine embryo cryopreservation in a chemically defined medium
A. Østergaard, L. Gavin-Plagne, M. Guedes Teixeira, S. Buff and T. Joly
pp. 142-142
RDv32n2Ab3333 Assessment of spindle morphology and reactive oxygen species production after vitrification of bovine oocytes following in vitro maturation in the presence of glutathione ethyl ester
T. García-Martínez, M. Vendrell-Flotats, I. Martínez-Rodero, M. Álvarez-Rodríguez, M. López-Béjar and T. Mogas
pp. 142-142
RDv32n2Ab3434 Effect of polysaccharide from Flammulina velutipes on the vitrification of bovine oocytes
Y. Ihara, K. Tatakura, Y. Wada, H. Kawahara and K. Yamanaka
pp. 143-143
RDv32n2Ab3535 Ethanolic extracts of Cerrado plants in cryotolerance of in vitro-produced bovine embryos
A. A. G. Fidelis, G. O. Fernandes, T. S. Kawamoto, F. R. Melo and M. A. N. Dode
pp. 143-143
RDv32n2Ab3636 Extended culture after vitrification-warming helps in spindle recovery of bovine oocytes
E. Gutierrez, Z. Jiang and K. Bondioli
pp. 144-144
RDv32n2Ab3737 In vitro maturation and fertilization in white-tailed deer (Odocoileus virginianus) oocytes vitrified with trehalose or sucrose
V. A. Rubio-Santillanes, J. Antillón-Ruiz, F. A. Rodríguez-Almeida, S. Romo, H. Álvarez-Gallardo, J. L. Rodríguez-Suástegui, E. Hernández-Pichardo and M. E. Kjelland
pp. 144-144
RDv32n2Ab3838 Ram sperm longevity after cryopreservation in extender containing L-carnitine
C. Souza, F. Brandão, J. Santos, V. Alfradique, V. Brair, L. Prellwitz, P. S. Rangel, A. Silva and J. M. Souza-Fabjan
pp. 145-145
RDv32n2Ab3939 Nanowater enhances cryoprotective effects of glycerol during ram semen freezing
J. Szymanowicz, M. Murawski, T. Schwarz and P. Bartlewski
pp. 145-145
RDv32n2Ab4040 Comparison of slow and rapid freezing in freeze-dried ram spermatozoa
L. Palazzese, D. A. Anzalone, P. Toschi and P. Loi
pp. 146-146
RDv32n2Ab4141 Effect of vitrification on DNA integrity of human spermatozoa
Y. Toishibekov, B. Shalekenov, Y. Assanova, S. Shalekenov, Y. Kuandykov, D. Toishybek and V. Isachenko
pp. 146-146
RDv32n2Ab4242 Vitrification of in vitro-produced feline embryos
D. Fuller, J. Herrick, J. Graham and J. Barfield
pp. 146-147
RDv32n2Ab4343 Actions of colony-stimulating factor 3 on the maturing oocyte and developing embryo in cattle
E. Jannaman, Y. Xiao and P. Hansen
pp. 147-147
RDv32n2Ab4444 Analysis of abnormal chromatin configuration induced by inhibiting MEK at the 1-cell stage
K. Magara, S. Naruto, R. Watanabe, T. Wakayama and S. Kishigami
pp. 147-148
RDv32n2Ab4545 Expression patterns of PRDM family genes in porcine pre-implantation embryos
K. Farrell, K. Uh and K. Lee
pp. 148-148
RDv32n2Ab4646 Presence of porcine TET3L isoform in oocytes: Potential involvement in the DNA demethylation process
K. Uh, N. Wax, K. Farrell and K. Lee
pp. 148-148
RDv32n2Ab4747 Inhibition of endoplasmic reticulum stress during in vitro maturation improves the developmental competence of bovine cumulus-oocyte complexes
H. Khatun, Y. Ihara, K. Takakura, Y. Wada and K.-I. Yamanaka
pp. 149-149
RDv32n2Ab4949 Expression and actions of the dickkopf-1 receptors KREMEN1 and KREMEN2 in the bovine pre-implantation embryo
T. Fernandes Amaral, Y. Xiao, E. Estrada-Cortes and P. Hansen
pp. 149-150
RDv32n2Ab5050 Effect of colony-stimulating factor 2 on competence of bovine blastocysts to survive vitrification
F. Sosa and P. J. Hansen
pp. 150-150
RDv32n2Ab5151 Bovine embryos with distinct early morphokinetic pathways present different post-embryonic genome activation transcriptomic patterns and different cryotolerance
A. P. Reis, A. Jampy, A. Teste, B. M. Le Guienne, L. Laffont, S. Ruffini, E. Canon, C. Archilla, L. Jouneau, A. Trubuil and V. Duranthon
pp. 151-151
RDv32n2Ab5252 Blocking of embryonic development by nanoparticles derived from endometrial and oviductal cells isolated with an Amicon filter system
J. Cabezas, D. Rojas, B. Melo-Baez, M. Gutierrez, F. Castro and L. Rodriguez-Alvarez
pp. 151-151
RDv32n2Ab5353 Transcriptomic profiles of uniform populations of in vivo-produced spherical, ovoid, or tubular porcine embryos during the initiation of elongation
S. Walsh, J. R. Miles, E. C. Wright-Johnson, B. Keel, L. A. Rempel and A. K. Pannier
pp. 152-152
RDv32n2Ab5454 Choline alters the pattern of DNA methylation and lipid content of pre-implantation bovine embryos
E. Estrada-Cortés and P. J. Hansen
pp. 152-152
RDv32n2Ab5555 Identification of microRNAs associated with sex determination in bovine amniotic fluid and maternal blood plasma
J. M. Sánchez, I. Gómez-Redondo, J. A. Browne, B. Planells, A. Gutiérrez-Adán and P. Lonergan
pp. 153-153
RDv32n2Ab5656 Mammalian pre-implantation embryos at the single cell level: The bovine as a model for early human embryonic development
N. Ortega, V. Ahola, A. Plaza-Reyes, J. Schell, P. Kumar, A. Jouneau, V. Duranthon and F. Lanner
pp. 153-153
RDv32n2Ab5757 Primordial germ cell distribution in the horse fetal gonad
D. Scarlet, U. Reichart, G. Podico, R. Ellerbrock, I. Canisso, I. Walter and C. Aurich
pp. 154-154
RDv32n2Ab5858 Effect of different light sources on the developmental capacity of bovine embryos produced in vitro
A. Gonzalez, F. Dobener, S. Chatterjee and C. Wrenzycki
pp. 154-154
RDv32n2Ab5959 Genome activation in intracytoplasmic sperm injection-derived horse embryos
D. E. Goszczynski, P. Tinetti, Y. H. Choi, K. Hinrichs and P. J. Ross
pp. 155-155
RDv32n2Ab6060 CRISPR-on, a new tool for activation of endogenous gene expression in bovine embryos
V. Savy, V. Alberio, N. Canel, L. Ratner, M. Gismondi, O. Taboga, S. Ferraris, S. Rulli, R. Fernandez-Martin, R. Bevacqua and D. Salamone
pp. 155-155
RDv32n2Ab6161 Low plasma progestin concentration during the early luteal phase impairs equine conceptus development until placentation
C. Okada, M. Kaps, S. Handschuh, D. Scarlet and C. Aurich
pp. 156-156
RDv32n2Ab6262 Peripheral transcriptome response to embryo mortality in Holstein cows
C. L. Gonzalez-Berrios, H. M. Georges, J. V. Bishop, H. Van Campen, M. G. Thomas and T. R. Hansen
pp. 156-156
RDv32n2Ab6363 Inhibition of CXCR4 at the fetal-maternal interface during placentation results in altered production of vascular endothelial growth factor receptors in the placenta on Day 90 of pregnancy
J. M. Ervin, S. Z. McIntosh, C. L. Runyan and R. L. Ashley
pp. 157-157
RDv32n2Ab6464 Prediction of pregnancy and early embryo loss through OAS-1 expression, concentrations of pregnancy-associated glycoproteins, and Doppler ultrasonography in beef cattle
G. D. Melo, B. P. Mello, L. M. F. Pinto, A. Guimarães, C. C. Rocha, I. G. Motta, E. H. Madureira, J. C. Silveira, K. G. Pohler and G. Pugliesi
pp. 157-157
RDv32n2Ab6565 Effect of oviductal fluid extracellular vesicle supplementation during in vitro culture on development and quality of bovine embryos
D. Le Bourhis, S. Janati Idrissi, P. Mermillod, A. Carmen, P. Salvetti and M. Saint Dizier
pp. 158-158
RDv32n2Ab6666 Lipid profiling of bovine blastocysts produced in vitro with and without a stimulator of cyclic guanosine monophosphate synthesis by multiple reaction monitoring profiling
L. Schefer, L. M. Naves, C. Ramires Ferreira, E. Pradebon da Silva, T. J. Sobreira, P. Vieiro Marchioretto, C. Lima Verde Leal, M. Rubessa and M. B. Wheeler
pp. 158-159
RDv32n2Ab6767 Effects of phytohemagglutinin on the culture of isolated bovine blastomeres derived from the 8-cell stage in vitro-produced embryos
Y. Hashiyada, Y. Aikawa, H. Matsuda and T. Yamanouchi
pp. 159-159
RDv32n2Ab6868 Anti-Mullerian hormone levels in different bovine species and the relationship with in vitro embryo production
J. Berdugo, A. Tarazona-Morales, J. Echeverry-Zuluaga, W. Cardona Maya, A. López-Herrera, G. Crudeli, J. Konrad and G. Espasandin
pp. 159-160
RDv32n2Ab6969 Phosphorylation of mechanistic target of rapamycin in porcine blastocyst-stage embryos is dependent on the concentration of glutamine in the medium
P. Chen, L. Spate and R. Prather
pp. 160-160
RDv32n2Ab7070 Trolox during in vitro maturation of bovine oocytes protects developing embryos from palmitic acid-induced lipotoxicity
J. De Bie, W. Marei, P. Bols and J. Leroy
pp. 161-161
RDv32n2Ab7171 Comparison of sexed semen ULTRA-4M with conventional semen for the in vitro production of bovine embryos
H. Alvarez, M. Kjelland, F. Villaseñor, M. Pérez and S. Romo
pp. 161-162
RDv32n2Ab7272 In vitro embryo production using prepubertal calf oocytes with conventional semen and sexed semen ULTRA-4M
A. Velazquez, H. Alvarez, M. Kjelland, F. Villaseñor, G. Ariza and S. Romo
pp. 162-162
RDv32n2Ab7373 Cytokine addition does not increase developmental competence of in vitro-produced bovine embryos
C. M. Helland, M. Barcelo-Fimbres and L. F. Campos-Chillon
pp. 162-163
RDv32n2Ab7474 Analysis of chromosomal abnormality of bovine IVF embryos based on next-generation sequencing
M. Okada, Y. Nagai, S. Matoba, Y. Sakuraba and S. Sugimura
pp. 163-163
RDv32n2Ab7575 Expression of lipid metabolism-related genes in bovine embryos cultured in vitro with diacylglycerol acyltransferase-1 inhibitor
K. Cañón-Beltrán, J. Giraldo-Giraldo, Y. N. Cajas, N. Vásquez, C. L. V. Leal, A. Gutiérrez-Adán, M. E. González and D. Rizos
pp. 163-164
RDv32n2Ab7676 Extracellular vesicles from oviduct and uterus in sequential culture improve the quality of bovine embryos produced in vitro
C. Leal, K. Cañon-Beltrán, Y. Cajas, P. Gallego, P. Beltrán-Breña, M. Hamdi, M. González and D. Rizos
pp. 164-164
RDv32n2Ab7777 Nobiletin supplementation affects gene expression profiles of the Akt pathway in bovine embryos in vitro
Y. N. Cajas, K. Cañón-Beltrán, C. L. V. Leal, M. E. González, A. Gutierrez-Adán and D. Rizos
pp. 164-165
RDv32n2Ab7878 Radio electric asymmetric conveyer treatment during prolonged cold storage of ovaries enhances developmental competence of oocytes in the domestic cat model
F. Ariu, E. Sanna Passino, A. Piras, V. Melosu, M. Maioli, A. Castagna, V. Fontani, S. Rinaldi and L. Bogliolo
pp. 165-165
RDv32n2Ab7979 MicroRNAs of extracellular vesicles secreted by embryos as an early biomarker of competence
B. Melo-Baez, Y. S. Wong, J. Cabezas, C. J. Aguilera, F. O. Castro and L. Rodriguez-Alvarez
pp. 166-166
RDv32n2Ab8080 Evaluation of extracellular vesicles from culture medium of human embryos as a possible method of pre-implantation genetic diagnosis
C. Aguilera, D. Veraguas, C. Henriquez, A. Velasquez, F. O. Castro and L. Rodriguez-Alvarez
pp. 166-166
RDv32n2Ab8181 Linoleic acid required for reduction of apoptosis through nuclear transcription factor-kappa B during pig embryo development
D. Lee, K. Choi, J. Oh, S. Kim, M. Lee, M. Cho and C. Lee
pp. 167-167
RDv32n2Ab8282 Stage-specific expression of lineage marker genes and pluripotency marker distribution in porcine pre-implantation embryos
J. Oh, M. Lee, D. Lee, K. Choi, S. Kim, M. Cho and C. Lee
pp. 167-167
RDv32n2Ab8383 Validation of propidium iodide dye for live-dead staining of bovine blastocysts: Preliminary results
H. Hellmold, D. Teuteberg, J. Tetens and C. Blaschka
pp. 168-168
RDv32n2Ab8484 Folate-methionine cycle and folate transport in developing buffalo embryos
S. Ansari, S. Jamwal, S. Saini, R. Singh and D. Malakar
pp. 168-168
RDv32n2Ab8585 Regulation of mammalian target of rapamycin signaling post-fertilisation is essential for efficient development of bovine pre-implantation embryos
M. El Sheikh, A. Mesalam, A. Mesalam, K. Lee and I. Kong
pp. 169-169
RDv32n2Ab8686 Effect of in vitro culture conditions on mitochondria functions in mouse embryos
M. Czernik, D. Winiarczyk, S. Sampino, P. Greda, J. A. Modlinski and P. Loi
pp. 169-169
RDv32n2Ab8787 Analyzing metabolomic profile of bovine IVF and somatic cell nuclear transfer embryos through Raman spectroscopy
J. Keim, W. Zhang, Y. Liu, H. Rutigliano, A. Zhou and I. Polejaeva
pp. 169-170
RDv32n2Ab8888 MicroRNA profile of in vitro bovine embryos cultured in the presence of amniotic extracellular vesicles shifts toward in vivo-collected blastocysts
A. Lange-Consiglio, B. Lazzari, F. Pizzi, A. Idda, F. Cremonesi and E. Capra
pp. 170-170
RDv32n2Ab8989 Embryonic metabolism orchestrates epigenetic mechanisms: What can we anticipate from the first cleavages?
J. Ispada, A. M. Fonseca Junior, E. C. dos Santos, K. Annes, O. L. R. Santos, C. B. de Lima, J. L. Chitwood, P. J. Ross and M. P. Milazzotto
pp. 170-171
RDv32n2Ab9090 Establishment and characterization of Day 30 equine chorionic girdle and allantochorion cell lines
S. Salman, A. Asghar, C. Magee, Q. Winger, G. Bouma and J. Bruemmer
pp. 171-171
RDv32n2Ab9191 In vivo- and in vitro-produced bovine embryos have different microRNA profiles after in vitro individual culture
A. Bridi, I. Motta, G. Andrade, M. Del Collado, A. Ávila, L. Silva, G. Pugliesi, F. Meirelles, J. Silveira and F. Perecin
pp. 171-172
RDv32n2Ab9292 Using physical parameters of bovine zygotes to predict in vitro development success
C. L. Timlin, A. Lynn, L. K. Wooldridge, K. Uh, A. D. Ealy, R. R. White, K. Lee and V. R. G. Mercadante
pp. 172-172
RDv32n2Ab9393 Efficient introduction of green fluorescent protein-9R, a protein with cell-penetrating peptides, into oocytes using intracytoplasmic sperm injection
R. Watanabe, H. Okaji, K. Magara, K. Tetsuka, T. Kaitsuka, K. Tomizawa and S. Kishigami
pp. 172-173
RDv32n2Ab9494 Efficient generation of myostatin mutation in Korean beef cattle
G. Gim, Y. Jin, S. Yum, H. Park, J. Lee, S. Koo, W. Lee and J. Goo
pp. 173-173
RDv32n2Ab9595 Obtaining birds with chimeric gonads using in vitro lentiviral transduction of primordial germ cells
K. A. Glumakova, O. N. Mityaeva, E. N. Antonova, O. V. Glazova, A. S. Komarchev and P. Y. Volchkov
pp. 173-173
RDv32n2Ab9696 Evaluation of CRISPR/Cas9 alternative delivery in parthenogenetic porcine embryos
O. Briski, G. La Motta, D. Salamone, R. Fernandez-Martin and L. Ratner
pp. 174-174
RDv32n2Ab9797 Loss of aggregation capacity of bovine in vitro-produced embryos and blastocyst-derived trophoblasts from Day 6 of development
V. Alberio, M. Yauri Felipe and D. Salamone
pp. 174-174
RDv32n2Ab9898 Effects of maternal age on oxygen consumption of oocytes and in vitro-produced equine embryos
G. Catandi, Y. Obeidat, J. Stokes, A. Chicco, T. Chen and E. Carnevale
pp. 175-175
RDv32n2Ab9999 Short-term storage of equine embryos at 5 or 20°C does not cause lipid peroxidation
G. D. Gastal, D. Scarlet and C. Aurich
pp. 175-175
RDv32n2Ab100100 Relationship between corpus luteum blood flow evaluated via colour Doppler ultrasound and pregnancy rate in bovine embryo transfer recipients
M. K. Sermersheim, B. R. Lindsey, L. M. Naves, M. Rubessa and M. B. Wheeler
pp. 176-176
RDv32n2Ab101101 Treatment with gonadotrophin-releasing hormone on Day 7 or 21 does not reduce pregnancy loss in dairy heifers receiving in vitro-produced embryos
B. J. Duran, R. V. Sala, P. L. J. Monteiro, C. Gamarra, M. Fosado, E. Walleser, A. Prata, R. Gennari, J. F. Moreno, M. C. Wiltbank and A. Garcia-Guerra
pp. 176-177
RDv32n2Ab102102 A delay in maternal zygotic transition may lead to early embryonic loss in poor-quality bovine blastocysts
H. Georges, J. Bishop, H. Van Campen, J. Barfield and T. Hansen
pp. 177-177
RDv32n2Ab104104 SOFaaci-HEPES or holding media can be used for embryo loading without changes in pregnancies per embryo transfer nor pregnancy loss in an in vitro-produced embryo transfer program
D. Pereira, D. Moreno, R. Sala, L. Carrenho-Sala, M. Fosado, J. Moreno and A. Garcia-Guerra
pp. 178-178
RDv32n2Ab105105 Optimization of a five-day fixed-time embryo transfer program in dairy heifers: Use of gonadotrophin-releasing hormone at initiation of the protocol
R. Sala, L. Carrenho-Sala, V. Absalon-Medina, A. Lopez, M. Fosado, J. Moreno, M. Wiltbank and A. Garcia-Guerra
pp. 179-179
RDv32n2Ab106106 Comparative growth rates and haematological parameters from calves born by transfer of vitrified in vitro-produced embryos and stepbrother calves born by AI
J. S. Lopes, C. Soriano-Úbeda, L. Sarrias-Gil, E. París-Oller, S. Navarro-Serna, R. Latorre and P. Coy
pp. 179-180
RDv32n2Ab107107 Follicular wave synchronization and FSH stimulation prior to ovum pickup for in vitro embryo production
L. Ferré, M. Kjelland, T. Stroud and P. Ross
pp. 180-180
RDv32n2Ab108108 Use of seminal plasma as ovulation inductor in alpacas (Vicugna pacos) embryo recipient and its effect on pregnancy rate
W. Huanca, K. Garcia, W. F. Huanca, A. Cordero and J. Malaga
pp. 181-181
RDv32n2Ab109109 Effect of ruminal infusion with propylene glycol on the in vitro embryo production of Holstein (Bos taurus) prepubertal heifers and pregnancy rate of the embryo transfer
R. Tosta, R. Sala, D. Pereira, D. Kendall, F. Elliff, J. Ziemer, E. Adelsberger, J. Moreno, B. Catussi and P. Baruselli
pp. 181-181
RDv32n2Ab110110 Absence of transmission of Mycoplasma bovis via naturally contaminated semen during in vitro fertilization
J. Peippo, N. Vähänikkilä, M. Mutikainen, H. Lindeberg, T. Pohjanvirta, H. Simonen, S. Pelkonen and T. Autio
pp. 182-182
RDv32n2Ab111111 Fertilizing ability of frozen and freeze-dried semen following intracytoplasmic sperm injection of in vitro-matured sheep oocytes
I. Menéndez-Blanco, F. Ariu, A. Piras, S. Nieddu, M. Paramio, A. Arav, S. Ledda and L. Bogliolo
pp. 182-183
RDv32n2Ab112112 The effect of biological extenders on in vitro induction of the acrosome reaction in bovine spermatozoa
L. Gatenby and K. R. Bondioli
pp. 183-183
RDv32n2Ab113113 Effect of antioxidants on motility and fertility of liquid-stored bovine sperm
Y. Honkawa, T. Fujikawa, N. Miura and C. Kubota
pp. 183-184
RDv32n2Ab114114 Omega-3-enriched diet improves fertilization competence of cryopreserved sperm
D. Kalo, D. Reches, A. Komsky-Elbaz, U. Moallem, Y. Zeron and Z. Roth
pp. 184-184
RDv32n2Ab115115 In vivo confocal laser endomicroscopy visualisation of fresh and frozen bull spermatozoa in the genital tract of dairy heifers
C. Richard, X. Druart, T. Saint-Beuve, V. Gelin, L. Laffont, S. Ruffini, S. Ghazali, M. Saint-Dizier, V. Duranthon, O. Sandra, B. Grimard and V. Mauffre
pp. 184-185
RDv32n2Ab116116 Effect of different energy sources on motility and calcium ionophore-induced acrosome reaction in equine sperm
L. Ramírez-Agámez, I. Ortíz, C. Hernández-Avilés, C. Love and K. Hinrichs
pp. 185-185
RDv32n2Ab117117 Supplementation of IVF medium with nerve growth factor improved bovine embryonic cleavage rates during summer months
E. Amiss, J. W. Stewart, V. M. Negrón-Pérez, K. Jones, H. Haines, M. L. Rhoads, F. S. Lima and J. L. Stewart
pp. 185-186
RDv32n2Ab118118 Semen quality and fertilization ability of myostatin-knockout boars
M.-F. Xuan, S.-Z. Han, B.-H. Quan, X.-J. Yin and J.-D. Kang
pp. 186-186
RDv32n2Ab119119 Short spermatozoa-oocyte co-incubation improves outcomes of IVF in sheep
D. Anzalone, M. Czernik, L. Palazzese, Y. Ressaissi, P. Scapolo and P. Loi
pp. 186-186
RDv32n2Ab120120 Use of fixable dyes to analyze equine sperm membrane integrity and acrosome reaction after A23187 treatment
I. Ortiz, M. Felix, H. Resende, C. Love and K. Hinrichs
pp. 187-187
RDv32n2Ab121121 Effect of pentoxifylline on motility of good- and poor-quality frozen-thawed equine sperm
M. Felix, I. Ortiz, H. Resende, J. Brom-de-Luna, C. Love and K. Hinrichs
pp. 187-187
RDv32n2Ab122122 Proteome of extracellular vesicles from follicular fluid of bovine 3- to 6-mm follicles: Similarity and specificity compared with granulosa cells
S. Uzbekova, C. Alminana-Brines, V. Labas, L. Combes-Soia, I. I. Kireev, A.-P. Teixeira-Gomes, R. E. Uzbekov and G. N. Singina
pp. 188-188
RDv32n2Ab124124 Ovarian follicular development and steroid secretion during oestrous cycle of Lohi sheep
M. Younis, M. Irfan-ur-Rehman Khan, A. Murtaza, M. Abbas, M. Z. Tahir, K. Javed, I. Mohsin and M. Shahzad
pp. 189-189
RDv32n2Ab125125 Transcriptomic changes in bovine ovarian cortex in response to FSH signaling
J. Candelaria, B. Rabaglino and A. Denicol
pp. 189-189
RDv32n2Ab126126 Subcortical maternal complex (SCMC) expression during folliculogenesis is affected by oocyte donor age in sheep
D. Bebbere, A. Abazari-Kia, F. Ariu, L. Bogliolo and S. Ledda
pp. 190-190
RDv32n2Ab127127 Chemokine receptor 2 (CCR2) is expressed in growing oocytes, and its deficiency affects follicular activation and long-term female fertility in mice
A. G. A. Santos, L. A. A. C. Pereira, R. C. Russo, J. H. M. Viana and P. H. A. Campos-Junior
pp. 190-190
RDv32n2Ab128128 Study of preantral ovarian follicular population in fetal alpaca (Vicugna pacos) ovaries
D. Dipaz-Berrocal, G. Rojas, C. Mamani and E. Mellisho
pp. 191-191
RDv32n2Ab129129 Ovarian dynamics and gonadotropins during selection of the dominant follicle in postpartum lactating versus non-postpartum cycling mares
M. Pastorello, M. O. Gastal, G. K. Piquini, D. B. Godoi and E. L. Gastal
pp. 191-191
RDv32n2Ab130130 Increased antral follicle population at 60 days of gestation in Holstein cows with low antral follicle count
R. G. Droher, F. Morotti, A. Guidugli Lindquist, A. Fonseca Zangirolamo and M. Marcondes Seneda
pp. 192-192
RDv32n2Ab132132 Introduction of F508del human mutation into the CFTR gene of sheep fetal fibroblasts using CRISPR/Cas9 ribonucleoprotein
I. Viotti Perisse, Z. Fan, A. Van Wettere, Z. Wang, A. Harris, K. White and I. Polejaeva
pp. 192-193
RDv32n2Ab133133 CRISPR/Cas9 gene editing of in vivo-fertilized bovine embryos via endoscopic oviductal flushing and electroporation of zygotes
D. Miskel, L. Beunink, M. Poirier, V. Havlicek, F. Rings, K. Schellander, U. Besenfelder and M. Hölker
pp. 193-193
RDv32n2Ab134134 Generation of NANOS2 knockout goats using CRISPR/Cas9 and somatic cell nuclear transfer techniques
Z. Fan, M. Regouski, Y. Liu, J. Keim, I. Perisse, J. Oatley and I. Polejaeva
pp. 193-194
RDv32n2Ab135135 Strategies for transfection of bovine mesenchymal stem cells with pBC1-anti-CD3 vector
F. B. Duarte, S. N. Báo, M. Brígido, J. M. Araújo, E. d. O. Melo and C. F. Martins
pp. 194-194
RDv32n2Ab136136 Effects of the homology direct repair enhancer RS-1 on pig embryo culture
C. Gomes Lucas, B. Bauer, P. Chen, L. Spate, K. Wells and R. Prather
pp. 194-194
RDv32n2Ab137137 Recovery of quail spermatogenesis by donor spermatogonia transplantation
A. N. Vetokh, E. K. Tomgorova, L. A. Volkova, N. A. Volkova and N. A. Zinovieva
pp. 195-195
RDv32n2Ab138138 Associations of sperm head morphometrics with quality parameters of frozen-thawed ram semen
J. Navaranjan, J. Szymanowicz, M. Murawski, T. Schwarz and P. M. Bartlewski
pp. 195-195
RDv32n2Ab139139 Heat stress has a deleterious effect on bull semen quality and subsequent embryo development
A. Seifi-Jamadi, H. Kohram, M. Zhandi, N. Llamas Luceño, B. Leemans, E. Henrotte, C. Latour, K. Demeyere, E. Meyer and A. Van Soom
pp. 196-196
RDv32n2Ab140140 The relationship between morphometric characteristics and semen parameters in Bapedi rams
A. Maqhashu, H. A. O'Neill, P. J. Sebei, F. V. Ramukhithi, N. O. Mapholi, K. A. Nephawe and T. L. Nedambale
pp. 196-196
RDv32n2Ab141141 Effect of combined treatment of melatonin and equine chorionic gonadotrophin on fresh semen quality of Beetal bucks during the non-breeding season
M. Abbas, M. Irfan-ur-Rehman Khan, A. Rehman, N. Hameed, I. Mohsin, M. Younis, M. Bilal and M. Shahzad
pp. 197-197
RDv32n2Ab142142 Metabolomic analysis of fresh and frozen bovine seminal plasma: A preliminary study
M. A. Kosior, N. Pagano, A. Staropoli, C. De Canditiis, V. Longobardi, G. Zullo, F. Vinale and B. Gasparrini
pp. 197-198
RDv32n2Ab143143 Stimulated glycolysis is able to maintain ATP levels and motility of bull spermatozoa submitted to mitochondrial depolarisation
J. Losano, J. Padín, I. Méndez-López, D. Angrimani, N. Montez, A. García, V. Barnabe and M. Nichi
pp. 198-198
RDv32n2Ab144144 Serum testosterone profile in Marwari stallions and its relationship with testicular parameters, semen characteristics, reaction time, stallion age, bodyweight, and height
A. K. Chaudhary, G. N. Purohit, J. S. Mehta, S. K. Ravi and T. R. Talluri
pp. 198-198
RDv32n2Ab145145 ProAKAP4 concentrations in semen as a predictive tool of bull fertility: A preliminary study
I. Ruelle, N. Seregeant, D. Bencharif, F. Charreaux, C. Thorin, S. Michaud, M. Dordas-Perpinyà, N. Jouy, S. Audry, C. Maurage, M. Delehedde and L. Briand-Amirat
pp. 199-199
RDv32n2Ab146146 Tributyltin chloride exposure alters ejaculated bull sperm function and embryo development
B. W. Daigneault and K. E. Latham
pp. 199-199
RDv32n2Ab147147 Dynamics of drake spermatogenesis
L. A. Volkova, A. N. Vetokh, E. K. Tomgorova, N. A. Volkova, H. V. Ashraf, V. A. Bagirov and N. A. Zinovieva
pp. 200-200
RDv32n2Ab148148 Bull spermatozoa uptake of extracellular vesicles from bovine seminal plasma
N. Pagano, M. A. Kosior, B. Gasparrini, V. Longobardi, C. De Canditiis, G. Albero, M. C. Deregibus, G. Bosi, A. Idda and A. Lange Consiglio
pp. 200-200
RDv32n2Ab149149 The second isoform of gonadotrophin-releasing hormone and its receptor affect boar semen quality
C. E. Ross, F. H. Choat, K. N. Plager, A. T. Desaulniers, R. A. Cederberg, G. A. Mills and B. R. White
pp. 201-201
RDv32n2Ab150150 α6 Integrin for evaluating sperm quality in bulls with different capacities of invitro embryo production
E. G. A. Perez, D. L. D'Ercole, M. H. Macedo, A. A. F. Rodrigues and R. F. Gonçalves
pp. 201-201
RDv32n2Ab151151 Influence of chorionic gonadotrophin, breeding procedure, and gonadotrophin-releasing hormone on pregnancy, embryo viability, and kidding rate of lactating Alpine goats time inseminated during the early transitional reproductive phase
M. Calle, L. Dawson, M. Rojas and E. Loetz
pp. 202-202
RDv32n2Ab152152 An effective method of inducing oestrus in superovulation-treated Japanese Black donor cows after egg collection
Y. Aoyagi, M. Takeuchi, T. Oono, K. Hayama, M. Urakawa, Y. Oono and M. Koiwa
pp. 202-203
RDv32n2Ab153153 The effects of parity and oxytocin or prostaglandin F2α added to insemination doses on reproductive performance of pigs bred in summer
T. Schwarz, P. Jaros, R. Tuz, J. Nowicki and P. M. Bartlewski
pp. 203-203
RDv32n2Ab154154 A method of oviductal semen deposition for use in the goat
N. Buzzell, S. Blash, K. Miner, M. Schofield, J. Pollock, N. Hawkins, M. Hevy and W. Gavin
pp. 203-204
RDv32n2Ab155155 Fertility of sexed semen in Holstein heifers and cows
M. Yamaguchi, M. Takayama, T. Nishisouzu, H. López and O. Dochi
pp. 204-204
RDv32n2Ab156156 Oestrus response, corpus luteum function, and pregnancy rates following aromatase inhibitor treatment in beef heifers
E. M. Zwiefelhofer, M. L. Zwiefelhofer, J. Singh, M. G. Colazo, G. A. Franco, S. T. Reese, K. G. Pohler and G. P. Adams
pp. 204-205
RDv32n2Ab157157 Effect of a slow-release gonadotrophin-releasing hormone analogue on ovarian activity and oestrous behaviour in mares
M. Kaps, C. Gautier, C. Cardoso Okada, J. Kuhl, J. Aurich and C. Aurich
pp. 205-205
RDv32n2Ab158158 Optimal time of AI and changes in vaginal mucus characteristics relative to the onset of standing oestrus in Beetal goats
A. Murtaza, M. Irfan-ur-Rehman Khan, M. Abbas, W. Ahmad, M. Z. Tahir and I. Mohsin
pp. 205-206
RDv32n2Ab159159 Laparoscopic insemination method in sheep allows the use of an animal protein-free and inexpensive freezing medium
L. Gavin-Plagne, L. Boyer, A. Baudot, M. Guedes Teixeira, G. Louis, L. Commin, S. Buff and T. Joly
pp. 206-206
RDv32n2Ab160160 Increasing gonadotrophin-releasing hormone dose at initiation of a 5-day CO-Synch protocol increases ovulatory response but not fertility in yearling beef heifers
E. Rojas Canadas, S. E. Battista, J. Kieffer, S. Wellert and A. Garcia Guerra
pp. 206-207
RDv32n2Ab161161 Increasing the dose of cloprostenol sodium reduced pregnancy losses but did not increase pregnancies per AI in lactating dairy cows
T. Minela, A. Santos, E. Schuurmans and J. R. Pursley
pp. 207-207
RDv32n2Ab162162 Detection of ovulation disorders and normal ovulation using wireless sensors of ventral tail surface temperature and neck acceleration data in Japanese Black cows
S. Matoba, M. Saito, K. Abe, S. Higaki and K. Yoshioka
pp. 207-208
RDv32n2Ab163163 Treatment with gonadotrophin-releasing hormone at the time of AI in beef heifers that fail to express oestrus after an estradiol-based synchronisation protocol improves pregnancies per AI
A. Butler, H. Butler, G. Cesaroni, R. Alberio, S. Perez Wallace and A. Garcia-Guerra
pp. 208-208
RDv32n2Ab164164 Gonadotrophin-releasing hormone injection and colour flow Doppler ultrasound of the preovulatory follicle as a tool to increase pregnancy outcome after timed AI in beef cows
L. Pfeifer, J. Andrade, E. Moreira, G. Silva, V. Souza, V. Nunes and L. Siqueira
pp. 209-209
RDv32n2Ab165165 Comparison of spermatozoa traits and testosterone levels, seminal plasma constituents, and lipid peroxidation of Large White × Landrace and Kolbroek boars
T. R. Netshirovha, R. S. Thomas, A. T. Kanengoni, M. B. Matabane, M. L. Mphaphathi, C. M. Pilane and M. Chimonyo
pp. 209-209
RDv32n2Ab166166 Comparison of the 7 and 7 Synch protocol and the 7-day CO-Synch + controlled internal drug release protocol among recipient beef cows in an embryo transfer programme
R. C. Bonacker, K. R. Gray, C. A. Breiner, J. M. Anderson, D. J. Patterson and J. M. Thomas
pp. 210-210
RDv32n2Ab167167 Improving the reproductive performance of beef cattle following multivalent viral-bacteria vaccination and mineral supplementation
G. Decuadro-Hansen, R. Silva, B. Lima, J. Lima, L. Durel, E. Silva and G. Macedo
pp. 210-211
RDv32n2Ab168168 Comparison of different Doppler ultrasound settings for pregnancy diagnosis based on corpus luteum perfusion at 21 days after AI in beef cattle
S. R. Wellert, S. E. Battista, J. Kieffer, R. N. Lurch and A. Garcia-Guerra
pp. 211-211
RDv32n2Ab169169 Comparison of pregnancy outcomes in dairy heifers artificially inseminated with sexed semen deposited in the uterine horns versus the uterine body
S. Kirks, R. Palomares, P. Melendez, M. Ferrer, A. Hoyos, J. Bittar, Z. Turner, M. Ibrahim, J. Gutierrez, D. Lopez, A. Gutierrez, V. Pattarajinda and J. Urdaneta
pp. 211-212
RDv32n2Ab170170 Embryonic loss and pregnancy rate in response to resynchronisation using oestradiol benzoate or injectable progesterone at 14 days after timed AI in Bos taurus × Bos indicus beef heifers
C. C. Vieira, V. Buss, A. M. Oliveira, M. E. R. Costa, B. G. Freitas, G. A. Pessoa, F. G. Leivas, B. M. Guerreiro, G. Pugliesi and F. S. Mesquita
pp. 212-212
RDv32n2Ab171171 Follicular and luteal characteristics and pregnancy rates in Bos indicus suckled cows treated with two oestradiol/progesterone-based protocols with a prolonged pro-oestrus
A. Cedeño, R. Maingón, J. P. Cedeño, C. Guadalupe, L. Morales, L. Pinargote, V. Figueroa and G. A. Bó
pp. 212-213
RDv32n2Ab172172 Effect of period of insertion of a progesterone-releasing device and pro-oestrus length on follicular and luteal characteristics and pregnancy rates to fixed-time AI in Bos indicus heifers
G. A. Bo, A. Cedeño, R. Maingón, J. P. Cedeño, H. Gamboa, J. Avellan, J. Bravo, C. Rivera and I. Macias
pp. 213-213
RDv32n2Ab173173 Pregnancy rates in suckled beef cows synchronised with progesterone/oestradiol-based protocol and inseminated with conventional or sexed-sorted semen
E. Huguenine, J. de la Mata, A. Menchaca, R. L. R. de Carneiro and G. A. Bo
pp. 214-214
RDv32n2Ab174174 Use of mesenchymal stem cell treatment to improve oocyte yield and in vitro embryo production in cattle
M. Peixer, P. Malard, J. Carvalho, M. Dode, J. Viana and R. Pogue
pp. 214-215
RDv32n2Ab175175 Use of equine chorionic gonadotrophin in a minimum-handling protocol for oocyte collection in bison
M. Zwiefelhofer, E. Zwiefelhofer, J. Singh, V. Wallace and G. Adams
pp. 215-215
RDv32n2Ab176176 Synchronisation of follicle wave emergence prior to superstimulation with purified FSH for ovum pickup affects blastocyst rate in pregnant Holstein heifers
L. Carrenho-Sala, M. Fosado, R. Sala, E. Peralta, D. Pereira, D. Moreno, J. Moreno and A. Garcia-Guerra
pp. 215-216
RDv32n2Ab177177 Collection of mouse immature oocytes using cilostazol, a phosphodiesterase 3A inhibitor treatment
A. Taiyeb, R. A. Jassim, A. Alazzam, M. Kjelland, C. K. Mawlood, T. Adams, A. Bani Younes and L. S. Salman
pp. 216-217
RDv32n2Ab178178 Evaluation of polar body extrusion following exposure of cattle oocytes to different concentrations of ethylene glycol cryoprotectant
M. L. Mphaphathi, M. Nkadimeng, S. M. Sithole, M. D. Sebopela, F. V. Ramukhithi, M. M. Tshabalala, A. Maqhashu, H. O'Neill and T. L. Nedambale
pp. 217-217
RDv32n2Ab179179 Bisphenol S affects in vitro early developmental oocyte competence in ewes
A. Desmarchais, O. Téteau, P. Papillier and S. Elis
pp. 217-218
RDv32n2Ab180180 Glucose versus fatty acids: Different energy supplies for the bovine oocyte
P. Lipinska and E. Warzych
pp. 218-218
RDv32n2Ab181181 The effects of aging in the oocyte induced by trichostatin A
H. A. Arena, E. Hicks, M. Mentler and B. D. Whitaker
pp. 218-219
RDv32n2Ab182182 Bisphenol A, but not bisphenol S, affects key microRNAs during bovine oocyte maturation
R. Sabry, M. Nguyen, L. Stalker, J. LaMarre and L. Favetta
pp. 219-219
RDv32n2Ab183183 Effects of epidermal growth factor and progesterone on in vitro oocyte growth, meiotic resumption, and expression of maturation-related transcripts in oocytes from bovine small antral follicles
J. R. V. Silva, F. T. G. Bezerra, L. R. F. M. Paulino, B. R. Silva and A. W. B. Silva
pp. 219-220
RDv32n2Ab184184 Effects of cyanidin supplementation on in vitro maturation of pig oocytes
E. Hicks, M. Mentler and B. D. Whitaker
pp. 220-220
RDv32n2Ab185185 Serum-dependent and serum-independent effects of prolactin and progesterone during the completion of in vitro maturation on metaphase II chromosomes in bovine oocytes
I. Lebedeva, G. Singina, E. Shedova and A. Lopukhov
pp. 220-221
RDv32n2Ab186186 Protective effect of resveratrol on in vitro fertilization of ovine oocytes matured under cadmium exposure
A. Piras, F. Ariu, N. Martino, A. Maltana, I. Menéndez-Blanco, M. Paramio, M. Dell'Aquila and L. Bogliolo
pp. 221-221
RDv32n2Ab187187 Effect of cytokines during in vitro maturation of bovine oocytes on the development potential of parthenogenetic embryos
G. N. Singina, E. N. Shedova, I. A. Polejaeva and T. E. Taradajnic
pp. 222-222
RDv32n2Ab188188 Improvement of bovine oocyte maturation in vitro through cytokine supplementation
K. S. Stoecklein, M. S. Ortega, L. Spate, C. N. Murphy and R. S. Prather
pp. 222-222
RDv32n2Ab189189 Effects of epidermal growth factor and progesterone on oocyte meiotic resumption and expression of maturation-related transcripts in bovine oocytes from medium-size antral follicles
L. G. Barrozo, F. T. G. Bezerra, L. R. F. M. Paulino, A. W. B. Silva and J. R. V. Silva
pp. 223-223
RDv32n2Ab190190 Effect of TCM-199 and synthetic oviductal fluid medium supplemented with varying hormone concentrations on in vitro maturation of canine oocytes
M. Tscharke, K. Kind, J. Kelly and J. Len
pp. 223-223
RDv32n2Ab191191 Effect of the time of oocyte collection through slicing method on meiosis resumption and in vitro embryo production
S. Soto-Heras, A. Lorenzo, I. Menéndez-Blanco, D. Izquierdo and M. Paramio
pp. 224-224
RDv32n2Ab192192 The effect of in vitro maturation on the PI3K-Akt pathway in bovine cumulus cells
G. Andrade, M. Del Collado, R. Nociti, W. J. Da Silva, F. Meirelles, J. Da Silveira and F. Perecin
pp. 224-224
RDv32n2Ab193193 Effect of clinical metritis on oocyte recovery, oocyte quality, and early in vitro developmental competence of embryos in Bos indicus dairy cattle
M. Saleem, Z. Sarwar, M. Saad, I. Zahoor, N. Ahmad and A. Riaz
pp. 225-225
RDv32n2Ab194194 Extracellular vesicles derived from ampullary oviductal fluid improve developmental competence of bovine oocytes
A. Asaadi, K. Pavani, N. Azari Dolatabadi, P. Van Damme and A. Van Soom
pp. 225-225
RDv32n2Ab195195 Lipid profiling of bovine oocytes matured with different stimulation of cyclic GMP synthesis by multiple reaction monitoring profiling
L. Schefer, L. M. Naves, C. Ramires Ferreira, E. Pradebon da Silva, T. J. Sobreira, P. Vieiro Marchioretto, C. L. V. Leal, M. Rubessa and M. B. Wheeler
pp. 226-226
RDv32n2Ab196196 Steroid concentrations in bovine follicular fluid are affected by metabolic stress
H. Aardema, H. T. A. Van Tol and P. L. A. M. Vos
pp. 226-227
RDv32n2Ab197197 The use of photostimulation to enhance oocyte cytoplasmic maturation
C. M. Checura, S. L. Pratt, L. V. Campbell, K. Farmer, G. Loughlin, M. Mitchell, A. Sandford, A. M. Treske and H. Malter
pp. 227-227
RDv32n2Ab198198 Comparison of in vitro production of bison and cattle embryos and effect of L-carnitine during maturation of bison oocytes
A. R. Moawad, H. Benham and J. P. Barfield
pp. 227-227
RDv32n2Ab199199 Strategies to improve canine oocyte in vitro maturation
C. Cittadini, M. Duque, A. De Stefano and D. Salamone
pp. 228-228
RDv32n2Ab200200 Maturation method affects lipid accumulation in bovine oocytes
O. A. C. Faria, T. S. Kawamoto, L. R. O. Dias, A. A. G. Fidelis, L. O. Leme, J. F. W. Sprícigo and M. A. N. Dode
pp. 228-228
RDv32n2Ab201201 Correlation between metaphase II oocyte cytoplasmic granulation patterns and intracytoplasmic sperm injection fertilization outcome in older patients
J. Hu, E. Lazzaroni-Tealdi, L. Zhang, D. Albertini, D. Barad and N. Gleicher
pp. 229-229
RDv32n2Ab202202 Proteomic, metabolomic and fatty acid composition in lactating and non-lactating mares’ uterine fluids
E. Derisoud, A. Hankele, L. Jouneau, C. Dubois, D. Rousseau-Ralliard, M. Dahirel, L. Wimel, S. Ulbrich and P. Chavatte-Palmer
pp. 229-230
RDv32n2Ab203203 High environmental temperatures during early fetal life may impair the ovarian reserve in cattle
S. Succu, S. Sale, G. Ghirello, J. Ireland, A. Evans, A. Atzori and F. Mossa
pp. 230-230
RDv32n2Ab204204 Discriminating intrauterine growth-restricted piglets through near-infrared spectroscopy of amniotic fluids
J. M. Feugang, M. Santos-Rivera, A. E. Grant, S. B. Park, D. Devos-Burnett, C. O. Lemley and C. K. Vance
pp. 230-230
RDv32n2Ab205205 Preliminary characterization of ovarian stem cells from bovine ovaries
A. Denicol, B. Weldon and L. Aguiar
pp. 231-231
RDv32n2Ab206206 Overexpression of germ cell genes DAZL, STRA8, and BOULE in bovine adipose tissue-derived mesenchymal stem cells for male germ cell derivation
P. Cordero, M. De los Reyes, V. H. Parraguez, M. Varas-Godoy, C. Torres and O. Peralta
pp. 231-231
RDv32n2Ab207207 Editing of prostaglandin E2 gene receptors EP2 and EP4 by CRISPR/Cas9 technology in equine adipose mesenchymal stem cells
A. C. Furlanetto Mançanares, J. Cabezas, D. Rojas, J. Manriquez, L. Rodriguez and F. Ovideo Castro
pp. 232-232
RDv32n2Ab208208 Effect of growth factors and reprogramming molecules on induction to multipotency of dermal fibroblasts from colocolo (Leopardus colocolo)
D. Echeverry, D. Rojas, C. Aguilera, L. Rodriguez-Alvarez and F. Castro
pp. 232-232
RDv32n2Ab209209 Rho signaling-directed regulation of YAP/TAZ in parthenogenetic stem cells
G. Pennarossa, S. Arcuri, F. Gandolfi and T. Brevini
pp. 233-233
RDv32n2Ab210210 Horse allogeneic mesenchymal stem cells perform homing and ameliorate endometrial inflammation after induced endometritis of mares
F. Navarrete, F. Saravia, G. Cisterna, F. Rojas, L. Rodríguez-Alvarez, D. Rojas and F. Castro
pp. 233-233
RDv32n2Ab212212 Increased expression of YAP/TAZ encourages outgrowth establishment and three-dimensional colony formation and boosts plasticity of parthenogenetic stem cells
S. Arcuri, G. Pennarossa, F. Gandolfi and T. Brevini
pp. 234-234
RDv32n2Ab213213 WNT inhibition by dickkopf WNT signalling pathway inhibitor 1 (DKK1) cannot replace IWR-1 during derivation of bovine embryonic stem cells
Y. Xiao, T. Amaral, P. Tribulo, K. Diffenderfer, P. Ross and P. Hansen
pp. 234-234
RDv32n2Ab214214 Different pluripotency maintenance supplements affect the reprogramming process and pluripotency state of bovine-induced pluripotent stem cells
R. Botigelli, N. Pieri, B. Bessi, R. de Castro, K. Recchia, A. de Souza, G. Barbosa, F. Meirelles, F. Bressan and M. Nogueira
pp. 235-235
RDv32n2Ab215215 In vitro evaluation of reprogramming at 20% or 5% oxygen tension in adult equine fibroblasts
R. V. G. de Castro, N. C. G. Pieri, R. Botigelli, B. W. Bessi, G. Barbosa, K. Recchia, M. D. C. Barrondo, B. M. Grizendi, R. G. S. Dória, P. Fantinato-Neto, J. M. Garcia and F. F. Bressan
pp. 235-236
RDv32n2Ab216216 Oxygen levels and pluripotency maintenance supplements affect cellular reprogramming of bovine fibroblasts
B. Bessi, R. Botigelli, K. Recchia, N. Pieri, G. Barbosa, R. de Castro, A. de Souza, F. Meirelles and F. Bressan
pp. 236-236
RDv32n2Ab217217 Induction and differentiation of porcine induced pluripotent stem cells into neuronal precursor cell-like cells
L. S. Machado, N. C. G. Pieri, R. C. Botigelli, R. V. G. Castro, A. F. de Souza, M. A. de Lima, A. Bridi, A. F. C. Andrade, F. V. Meirelles, K. K. Freude and F. F. Bressan
pp. 236-237
RDv32n2Ab218218 Urine samples as a noninvasive source of induced pluripotent cells in the swine model
K. Recchia, L. Machado, R. Botigelli, N. Pieri, P. Neto, F. Meirelles, A. Souza, S. Martins and F. Bressan
pp. 237-237
RDv32n2Ab219219 In vitro culture environment influences the ability to generate porcine primordial germ cell-like from induced pluripotent stem cells
N. Pieri, R. Botigelli, A. de Souza, K. Recchia, R. de Castro, F. Meirelles, F. Bressan and A. Andrade
pp. 237-238
RDv32n2Ab220220 Exploring the use of mesenchymal stem cells for treatment of mastitis and metritis in cattle
R. Singh, S. Saini, S. Ansari, S. Jamwal and D. Malakar
pp. 238-238
RDv32n2Ab221221 Isolation of turkey spermatogonia
E. K. Tomgorova, A. N. Vetokh, L. A. Volkova, N. A. Volkova, H. V. Ashraf, V. A. Bagirov and N. A. Zinovieva
pp. 238-239
RDv32n2Ab222222 Effective donor selection before superovulation treatment for Japanese Black beef cattle
N. Muraguchi, H. Uemura and C. Kubota
pp. 239-239
RDv32n2Ab223223 Multiple ovulation and embryo harvest in relation to energy balance in Latvian native cow breeds
A. Vanaga, I. Sematovica and O. Ponomarjova
pp. 239-240
RDv32n2Ab224224 Superstimulation effect on in vitro embryo production and relationship with anti-Müllerian hormone in buffaloes (Bubalus bubalis) aspirated 7 or 14 days after follicular ablation
J. Konrad, J. A. Berdugo Gutierrez, R. Yuponi, N. Vallejos, W. Cardona-Maya, G. Clérico, G. Crudeli and M. Sansinena
pp. 240-240
RDv32n2Ab225225 Evaluation of superovulatory response in Brazilian native sheep by B-mode ultrasonography
G. B. Vergani, M. S. D. Lima, K. M. Silva, A. W. U. Monteiro, A. F. Ramos, R. I. T. P. Batista, W. R. R. Vicente, M. E. F. Oliveira and J. F. Fonseca
pp. 240-241
RDv32n2Ab226226 Effect of the presence or absence of corpus luteum in females with small antral follicles at the beginning of a progesterone-based oestrus synchronization protocol on superovulatory response and embryo yields in sheep
M. S. D. Lima, G. B. Vergani, K. M. Silva, A. W. U. Monteiro, L. M. C. Pereira, L. M. C. Ramos, R. I. T. P. Batista, J. F. Fonseca, W. R. R. Vicente, M. E. F. Oliveira and D. I. A. Teixeira
pp. 241-241
RDv32n2Ab227227 Is the antral follicle count on a random day of the oestrous cycle correlated with superovulatory responses in Santa Inês ewes?
M. Pupin, G. Vergani, M. Lima, K. Silva, A. Monteiro, A. Ramos, R. Batista, W. Vicente, M. Oliveira and J. Fonseca
pp. 241-242
RDv32n2Ab228228 Effect of seminal plasma on the interval to application of equine chorionic gonadotrophin for the recovery of cumulus-oocyte complexes in alpacas (Vicugna pacos)
W. F. Huanca, J. M. Palomino, J. C. Villanueva, J. Malaga and W. Huanca
pp. 242-242
RDv32n2Ab229229 From head to tail: A red wolf sperm project
S. Kamen, J. Nagashima, N. Songsasen and M. Ferraz
pp. 242-243
RDv32n2Ab230230 Cryopreservation for Canis lupus conservation: Evaluating protocols to freeze grey wolf testicular tissue
C. Andrae, M. Ferraz, N. Songsasen and J. Nagashima
pp. 243-243
RDv32n2Ab231231 Novel protocol for the in vitro maintenance and expansion of adult epidermal LGR5+ stem cells
N. Gupta, K. Polkoff and J. Piedrahita
pp. 243-244
