296. Characterization of E74 like factor 3 in the murine pre-implantation embryo
E. B. Little A , J. L. Stanton A and D. P. L. Green ADepartment of Anatomy and Structural Biology, University of Otago, Dunedin, New Zealand
Reproduction, Fertility and Development 17(9) 125-125 https://doi.org/10.1071/SRB05Abs296
Submitted: 26 July 2005 Accepted: 26 July 2005 Published: 5 September 2005
Abstract
The E26 transformation specific (ETS) family of transcription factors is defined by a highly conserved DNA binding domain containing a hydrophobic core motif 5′-GGA[A/T]-3′. A subfamily of ETS factors, epithelium-specific ETS factor (ESE), includes E74-like factor 3 (Elf3). Elf3 is expressed in many organs including the eye, skin and gastrointestinal tract. By upregulating specific gene transcription, Elf3 is instrumental in causing these organs to become fully differentiated. The presence of Elf3 in the pre-implantation embryo was first reported in 2002.1 Data mining has suggested that the expression of Elf3 increases at the 16-cell embryo and blastocyst stages of pre-implantation development compared to the preceding stages. Reverse transcriptase–polymerase chain reaction (RT-PCR) was used to amplify the 3′ untranslated region (3′UTR) of Elf3. These results suggest that there are two isoforms of Elf3 present in the pre-implantation embryo, which differ by an 88 bp deletion/insertion. The expression and location of these isoforms was investigated using RT-PCR and in-situ hybridisation.
(1) Stanton and Green (2002) Mol. Hum. Rep. 8, 149–166.
