129 Effect of follicle wave synchronisation and follicle stimulating hormone treatment on in vitro embryo production in Bos indicus (Gyr) donors
A. V. Cedeño A B , B. Bernal A , L. Pinargote A , V. Ocampo A , B. Mendoza A and G. A. Bó B CA Instituto de Reproducción Animal Ecuador, Guayaquil, Guayas, Ecuador
B Universidad Nacional de Villa María, Villa del Rosario, Córdoba, Argentina
C Instituto de Reproducción Animal Cordoba, General Paz, Córdoba, Argentina
Reproduction, Fertility and Development 35(2) 192-192 https://doi.org/10.1071/RDv35n2Ab129
Published: 5 December 2022
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing on behalf of the IETS
An experiment was designed to evaluate the effect of follicle wave synchronisation and a single FSH administration on in vitro embryo production in Bos indicus donors. Purebred, mature, non-lactating, cycling, dairy (Gyr) donor cows (n = 40), with a body condition score between 3 and 4 (scale 1–5) were randomly divided to be superstimulated twice, with a 15-day interval, in a crossover design (all donors received two treatments, which were represented equally in each replicate). Donors in the EB + P4 + FSH group received an intravaginal device with 0.5 g of progesterone (P4; DIB, Zoetis), and 50 mg of P4 (Progestar, Syntex) plus 2 mg of oestradiol benzoate (EB, Gonadiol, Zoetis) intramuscularly (i.m.) on Day 0 and a single i.m. injection of Folltropin (Vetoquinol) diluted in saline (60 mg NIH FSH P1; 3 mL) on Day 4. On Day 7, in the afternoon (i.e. 36 h after FSH), P4 devices were removed and cows were submitted to an ultrasound-guided follicle aspiration (OPU). Cows in the untreated control group did not receive any treatments and were OPU at the same time. All the OPU were performed by a single operator and recovered cumulus-oocyte complexes (COCs) were classified, matured, and fertilised and presumptive zygotes were cultured in vitro for seven days using a commercial in vitro embryo-production system (Vitrogen, YVF Biotech). Frozen-thawed semen from a single bull was used for IVF. Data were analysed using the generalised linear and mixed model procedure using treatment and replicate as fixed variables and cow as a random variable. Although the mean (±s.e.m.) number of COCs collected by OPU did not differ among groups (P = 0.39), the number of viable COCs (P = 0.025), and viable (grade 1 and 2) blastocysts produced (P = 0.035) were greater in cows in the EB + P4 +FSH group than in the untreated control group (Table 1). In summary, the synchronisation of follicle wave emergence plus the administration of a single i.m. injection of FSH in saline increases the production of viable COCs and embryos for commercial in vitro embryo production in purebred Bos indicus (dairy Gyr) donors.
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