10 TRACEABILITY SYSTEM FOR AN INDIVIDUAL FROZEN SEMEN STRAW BY A TINY RADIO FREQUENCY IDENTIFICATION CHIP

Abstract

A traceability of frozen semen straws that certify the bloodline of a sire will improve the efficiency of cattle breeding. Semen of the sire can be generally identified by the colour of a straw and printing on a straw. However, it is difficult to identify individual frozen semen straws by the conventional method. To identify straws individually, each straw must be tagged with a unique ID that is able to be read quickly in the frozen state. We have established the identification method for individual frozen semen straws using radio frequency identification (RFID) and developed a traceability system that is able to record the distribution history of an individual semen straw from the production of frozen semen to artificial insemination (AI). We used a 2.5-mm diameter straw combined with an RFID tag, which consisted of a tiny RFID chip (μ-Chip: Hitachi Co. Ltd, 0.4 × 0.4 × 0.2 mm) and a thin aluminum antenna on the polyester sheet (54 × 1.5 × 0.05 mm). The μ-chip contains a unique ID (128 bit). It responds to the 2.45 GHz microwave frequency that is emitted from the RFID reader and transmits its ID to the RFID reader. The μ-Chip retains its function on a straw, which has been stored in the liquid nitrogen (–196°C). Artificial insemination of the frozen semen straw with the RFID tag could be performed using a conventional AI gun. The semen traceability system consisted of the database server and the client software. Information about semen straws is connected with their ID and stored on the database server. Producers and distributors of frozen semen can count the number of semen straws correctly by reading their ID one by one and can send information to the database server through the web by easy operation using the client software. Artificial insemination information can be instantly recorded by the handy terminal that is able to read both a bar-code on the ear tag of the cow and the RFID tag of the straw. The system has been used on fields at Gifu Prefecture in Japan for ∼3 months. Eight hundred thirty-one frozen semen straws with the RFID tag were produced from 3 bulls. After freezing of the straws, 17 straws were not identified with their ID. It was speculated that their μ-Chips were disconnected from the antenna during freezing. Six hundred ninety-four straws were distributed to the 7 AI centers. Five hundred thirty straws were used for AI by 15 inseminators to a total of 424 cows at the 101 farms. Although only one straw could not read its ID after AI, information of the remaining 529 straws were recorded on the database accurately. A strict traceability of frozen semen straws can be established using the individual identification of straws by RFID. Information stored in the database would be useful for the evaluation of sire and the reproductive management of cows.

This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (MAFF).