128 SERUM TRANSAMINASE ACTIVITIES IN ENDOMETRITIC, REPEAT BREEDER AND ESTRUS CROSSBRED COWS

Abstract

This study was carried out to determine the serum transaminase activities in endometritic, repeat breeder, and anestrus crossbred (Sahiwal Friesian) cows. A total of 60 blood samples were studied for enzyme activity in serum, with a Spectronic 20 using the commercial diagnostic kit adjusted at 546 nm wavelengths. The performance of liver and the intensity of liver damage were studied by iodine flocculation test. The highest mean (142.2 ± 2.10 μ/L) value of (GOT) activity was observed in the anestrus group, while the lowest mean (58.36 ± 2.1 μ/L) value of GOT was seen in the control group. Analysis of variance revealed a significant (P < 0.01) difference for GOT activity in anestrus compared to control, repeat breeder, and endometritic groups. The highest mean (18.45 ± 3.05 μ/L) value of GPT activities was recorded in repeat breeders while the lowest mean (11.81 ± 1.99 μ/L) value was noted in the control. The occurrence of moderate liver damage was 41.67%, whereas the prevalences of mild and normal damage were 41.67 and 16.66%, respectively. The highest activity (134.80 ± 1.27 μ/L) of GOT was recorded in cows with moderate liver damage, while the lowest activity (50.54 ± 2.72 μ/L) of GOT was observed in cows with normal livers. Mild liver-damaged animals had lower mean (62.13 ± 3.14 μ/L) GOT values compare to moderate liver-damaged animals. Analysis of variance revealed that the moderate liver-damaged group differed significantly in GOT activity from the normal and mild liver-damaged groups. The highest mean values of GOP (91.42 ± 2.8 μ/L) and GPT (20.83 ± 3.40 μ/L) activities were seen in cows with weak body condition, while lowest activities of GOP (82.56 ± 6.42 μ/L) and GPT (13.25 ± 1.55 μ/L) activities were recorded in animals with satisfactory body condition. When the milk yield was considered, the highest mean value of GOT (91.73 ± 3.59 μ/L) and GPT (16.09 ± 3.23 μ/L) activities were recorded in high yielder and the lowest mean values of GOP (72.58 ± 4.79 μ/L) and GPT (13.05 ± 1.99 μ/L) activities were observed in low yielder cows. Based on the findings of this study, it was concluded that although the occurrence of moderate fatty liver was 41.67%, this could be associated with the infertility condition of crossbred dairy cows. It is quite possible that cows showing mild liver damage were in recovery stage after severe or moderate liver damage, as the postpartum period in cross bred cows under study varied from 1.5 to 8 months.