118 TRANSFER OF IRON FROM MOTHER TO FETUS IN WATER BUFFALO: ERYTROPHAGOCYTOSIS AND UTEROFERRIN
F. Pereira A , F. Braga B , J.R. Kfoury Jr B , L. Oliveira B , P. Papa B , A.F. Carvalho C , A. Kohayagawa D , W. Hamlett E , S. Oloris B , M.L. Dagli B , F. Bazer F and M.A. Miglino BA Paulista State University (UNESP-Dracena), Sao Paulo, Brazil
B University of Sao Paulo (FMVZ-USP), Sao Paulo, Brazil
C UNIFEOB, Sao Paulo, Brazil
D Paulista State University (UNESP-Botucatu), Sao Paulo, Brazil
E Department of Anatomy and Cell Biology, Indiana University School of Medicine, Notre Dame, IN 46556, USA
F Center for Animal Biotechnology and Cenomics, Texas A&M University, Collete Station, TX 77843, USA. Email: fverechia@dracena.unesp.br
Reproduction, Fertility and Development 17(2) 209-210 https://doi.org/10.1071/RDv17n2Ab118
Submitted: 1 August 2004 Accepted: 1 October 2004 Published: 1 January 2005
Abstract
The transplacental transport of iron by uteroferrin (Uf), and the hemophagous areas in the water buffalo placenta were analyzed to clarify the mechanism of blood extravasation in the materno-fetal interface with consequent transfer of iron to the fetus through the trophoblastic erythrophagocytosis (Murai and Yamauchi 1986 Nippon Juigaki Zasshi 48, 75–88) and in the endometrial glands (Bazer et al. 1991 Exp. Hematol. 19 910–19 915). In the water buffalo placenta this mechanism remains unclear; uteroferrin is very important in this process because it is both an iron transporter and a progesterone-induced hematopoietic growth factor. Our objective was to characterize these hemophagous areas and the endometrium of the water buffalo, focusing in the materno-fetal transfer of iron. Small pieces of the placentomes and interplacentomal region of water buffaloes (Bubalus bubalis bubalis, n = 51) in all of the gestation periods were fixed in 4% paraformoldehyde, and 2.5% glutaraldehyde in PBS, processed, and stained for light and transmission electron microscopy to characterize the hemophagous areas and endometrial glands morphologically, histochemically (Perls, acid phosphatase reaction), and immunohistochemically with rabbit anti-pig uteroferrin antibody to confirm the iron transfer. The hemophagous areas were present in the placentome from 4–10-months-pregnant placentae. The Perls reaction for ferric iron staining was negative in the placentome and positive in the endometrial glands, and the acid phosphatase reaction to detect phagocytic activity was positive in the placentome as well as in the interplacentomal region. The uteroferrin immunohistochemical reaction was positive in the trophoblast, mainly in determined regions of the materno-fetal interface and in other points deep in the placentome, and the endometrial glands showed a strong reaction in the epithelium and in the lumina. The ultrastructure of the hemophagous areas revealed ingested erythrocytes inside the epithelial cells of trophoblasts, endocytic vesicles, and caveolae. The endometrial gland epithelium is of the columnar type with microvilli and basal nuclei. The results obtained mainly by histochemistry and immunohistochemistry indicated that the hemophagous areas and endometrial glands are very important sites for iron transfer in water buffalo, and are thus involved in the regulation of fetal hematopoiesis.
This work was funded by FAPESP.
