Quantitative proteomic analysis of heifer serum at the peri-implantation period using two complementary methods
Jimena Inés Ruiz Álvarez A , Juan Manuel Teijeiro A B , Caroline Evans C and Patricia Estela Marini
A *
A Laboratorio de Medicina Reproductiva, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
B Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Buenos Aires, Argentina.
C Department Chemical and Biological Engineering, Bioanalytical Facility, University of Sheffield, Sheffield, UK.
Reproduction, Fertility and Development 35(6) 416-421 https://doi.org/10.1071/RD22228
Published online: 27 February 2023
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Context: Systemic events resulting from mother-embryo relation at the peri-implantation period may result in specific changes to the protein composition of serum and thus provide a source of biomarkers for early detection of pregnancy.
Aims: We set out to use two different quantitative proteomic approaches to test this hypothesis by comparing heifer serum at the peri-implantation period to that from cycling heifers.
Methods: Two-dimensional electrophoresis (2-DE) and isobaric tags were used for relative and absolute quantitation (iTRAQ) of proteins.
Key results: These methods yielded complementary data indicating biomarker candidate proteins.
Conclusions: Different proteomic methods provide different and complementary information that needs to be analysed in order to consider proteins as potential biomarkers.
Implications: In order to characterise the proteome under specific conditions, the use of complementary techniques is advisable.
Keywords: bidimentional electrophoresis, biomarker, cow, iTRAQ, pregnancy, protein, proteome, serum biomarker.
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