Maternal protein restriction before and during pregnancy leads to a gestational day-dependent response of folliculogenesis in outbred mice
Lucas C. Cardoso A , Beatriz F. M. D. Costa A , Fernando Felicioni A B , Dirce R. Oliveira C , Marcelo V. Caliari D , Enrrico Bloise A , Hélio Chiarini-Garcia A and Fernanda R. C. L. Almeida
A E
+ Author Affiliations
- Author Affiliations
A Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, 31270-901, Belo Horizonte, MG, Brazil.
B School of Medicine, Atenas University Centre, Av. Prefeito Alberto Moura, 6000, 35701-383, Sete Lagoas, MG, Brazil.
C Department of Basic Life Sciences, Federal University of Juiz de Fora, Campus Governador Valadares, Av. Dr Raimundo Monteiro de Rezende, 330, Centro – Governador Valadares, MG, CEP 35010-177, Brazil.
D Department of Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, 31270-901, Belo Horizonte, MG, Brazil.
E Corresponding author. Email: falmeida@icb.ufmg.br
Reproduction, Fertility and Development 33(10) 655-664 https://doi.org/10.1071/RD21028
Submitted: 29 January 2021 Accepted: 30 April 2021 Published: 11 June 2021
Abstract
Knowledge of follicle development during pregnancy under experimental conditions could be a key factor to understanding maternal ovarian activity. Thus, this study evaluated the effects of maternal protein restriction before and during pregnancy on folliculogenesis. Swiss outbred female mice were allocated to either a control (CC; 20% protein) or treated (TT; 8% protein) group. Pregnant females were killed either on Gestational day (GD) 7.5 or GD17.5 and the ovaries were evaluated using histomorphometric and immunohistochemical methods. TT females showed higher feed and energy intakes, but lower bodyweight gain at GD17.5 (P < 0.05). They also had lower number of secondary follicles at GD7.5 and a higher proportion of primordial follicles at GD17.5 (P < 0.05). In addition, the areas of the secondary follicles and their granulosa layer were smaller in the TT group on GD7.5, whereas the areas of the oocyte and granulosa layer from atretic follicles were larger (P < 0.05). Notwithstanding the slight increase in the insulin-like growth factor 1 (IGF1) receptor expression on GD7.5 in the TT group, there was a marked reduction in IGF1 expression detected in secondary follicles on GD17.5 (P < 0.05). Collectively, these results demonstrate that protein restriction during pregnancy negatively affects follicle quality by reducing the size and activation capacity, which is more severe in late pregnancy.
Keywords: folliculogenesis, insulin-like growth factor 1 receptor (IGF1R), protein restriction, vascular endothelial growth factor 2 receptor (VEGF2R).
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