Molecular characterisation of oestrogen receptor ERα and the effects of bisphenol A on its expression during sexual development in the Chinese giant salamander (Andrias davidianus)
Yao Gao A , Chenhao Yang A , Huihui Gao A , Liqing Wang A , Changming Yang B , Hong Ji A and Wuzi Dong
A C
+ Author Affiliations
- Author Affiliations
A College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.
B Animal Husbandry and Veterinary Station of Chenggu County, Wenhua Road, Hanzhong, Shaanxi, 723200, China.
C Corresponding author. Email: dongwuzi@nwsuaf.edu.cn
Reproduction, Fertility and Development 31(2) 261-271 https://doi.org/10.1071/RD18107
Submitted: 18 March 2018 Accepted: 26 June 2018 Published: 10 August 2018
Abstract
The aim of this study was to characterise the molecular structure of the oestrogen receptor ERα and to evaluate the effect of bisphenol A (BPA) on ERα expression during sexual development of the Chinese giant salamander (Andrias davidianus). The ERα cDNA of A. davidianus includes an open reading frame of 1755 bp (encoding 584 amino acids), a 219-bp 5′ untranslated region (UTR) and a 611-bp 3′UTR. A polyadenylation signal was not found in the 3′UTR. Amino acid sequence analysis showed high homology between ERα of A. davidianus and that of other amphibians, such as Andrias japonicas (99.66% identity) and Rana rugose (81.06% identity). In 3-year-old A. davidianus, highest ERα expression was observed in the liver and gonads. During different developmental stages in A. davidianus (from 1 to 3 years of age), ERα expression in the testes increased gradually. ERα was localised in the epithelial cells of seminiferous lobules and in interstitial cells. ERα-positive cells were more abundant in the interstitial tissue during testicular development. ERα was located in the nucleus of oocytes during ovary development. We found that the sex of 6-month-old A. davidianus larvae could not be distinguished anatomically. The sex ratio did not change after larvae were treated with 10 μM BPA for 1 month. However, BPA treatment reduced bodyweight and ERα expression in the gonads in male larvae.
Additional keywords: gonad development, sexual differentiation.
References
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