Local effect of lysophosphatidic acid on prostaglandin production in the bovine oviduct
Yuka Yoshimoto A , Yoshihiko Kobayashi A , Izabela Woclawek-Potocka B , Emilia Sinderewicz B , Yuki Yamamoto A C , Koji Kimura A and Kiyoshi Okuda A
+ Author Affiliations
- Author Affiliations
A Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.
B Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
C Corresponding author. Email: yyamamoto@okayama-u.ac.jp
Reproduction, Fertility and Development 29(5) 1021-1028 https://doi.org/10.1071/RD15409
Submitted: 8 October 2015 Accepted: 3 February 2016 Published: 9 March 2016
Abstract
The mammalian oviduct plays an important role in the fertilisation and transport of gametes and embryo. Prostaglandins (PGs) are local mediators of oviductal functions and are involved in fertilisation and the transport of gametes and embryo. Lysophosphatidic acid (LPA), a kind of phospholipid, is involved in various physiological actions. We hypothesised that LPA regulates PG production in the bovine oviduct. To test this hypothesis, we examined the mRNA expression of LPA receptors (LPAR1–6) and LPA-producing enzymes (ATX, PLA1α, PLA1β) in ampullary and isthmic tissues and in cultured epithelial and stromal cells isolated from the bovine oviduct. We also investigated the effects of LPA on PG synthase expression and PG production in cultured cells. The mRNA of LPAR1–4, 6, ATX and PLA1α were expressed in cultured epithelial and stromal cells. The expressions of LPAR1–3 were significantly lower and the expression of LPAR4 was significantly higher in the isthmic than in the ampullary tissues. Lysophosphatidic acid significantly stimulated PG production in the cultured isthmic stromal cells. The overall findings suggest that LPA stimulates PG production via LPAR4 in the bovine oviduct. Since PGs are important for fertilisation and the transport of gametes and embryo, these findings show that locally produced LPA regulates oviductal functions.
Additional keywords: cultured cells, cyclooxygenase, smooth muscle movement, stromal cells.
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