Progestin priming before gonadotrophin stimulation and AI improves embryo development and normalises luteal function in the cat
Rosemary A. Stewart A B C H , Adrienne E. Crosier A , Katharine M. Pelican A D , Budhan S. Pukazhenthi A , Brandon D. Sitzmann B E , Tom E. Porter B , David E. Wildt A , Mary Ann Ottinger B F and JoGayle Howard A G
+ Author Affiliations
- Author Affiliations
A Center for Species Survival, Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, VA 22630, USA.
B Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA.
C Present address: Center for the Integrative Study of Animal Behavior, Indiana University, Bloomington, IN 47405, USA.
D Present address: Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.
E Present address: Office of Science and Technology, US Agency for International Development, Washington, DC 20523, USA.
F Present address: Department of Biology and Biochemistry, University of Houston, Houston, TX 77004, USA.
G Deceased.
H Corresponding author. Email: stewarra@indiana.edu
Reproduction, Fertility and Development 27(2) 360-371 https://doi.org/10.1071/RD13274
Submitted: 28 August 2013 Accepted: 26 October 2013 Published: 4 December 2013
Abstract
Exogenous gonadotrophins administered before AI can adversely alter endocrine dynamics and inhibit embryo development in felids. In the present study, we tested the hypothesis that priming the domestic cat ovary with progestin mitigates the negative influence of gonadotrophin therapy by normalising early embryogenesis and luteal function. Queens were given either: (1) progestin pretreatment plus chorionic gonadotrophins (n = 8; primed); or (2) gonadotrophins only (n = 8; unprimed). Ovulatory response was assessed laparoscopically, and cats with fresh corpora lutea (CL) were inseminated in utero. Ovariohysterectomy was performed 3 days later to recover intra-oviductal embryos for in vitro culture; one ovary was prepared for histology, and CL from the remaining ovary were excised and assessed for progesterone content and targeted gene expression. Of the six primed and seven unprimed queens inseminated, embryo(s) were recovered from five individuals per group. Embryos from progestin-primed donors more closely simulated normal stage in vivo development (P < 0.05). No 2- or 4-cell embryos from either group developed beyond 16-cells in vitro; however, 50% of unprimed and 66.7% of primed (P > 0.05) 5–16-cell embryos progressed to morulae or blastocysts by Day 4 of culture. Although histological characteristics were unaffected by progestin priming (P > 0.05), luteal progesterone was unusually high (P < 0.05) in unprimed compared with primed cats (72.4 ± 5.8 vs 52.2 ± 5.5 ng mg–1, respectively). Two genes associated with progesterone biosynthesis (luteinising hormone receptor and 3β-hydroxysteroid dehydrogenase) were upregulated in unprimed versus primed individuals (P = 0.05 and P < 0.05, respectively), indicating potential mechanistic pathways for the protective influence of pre-emptive progestin treatment. Building on earlier findings that progestin priming prevents spontaneous ovulation, increases ovarian sensitivity to gonadotrophins and ensures a normative endocrine environment, the present study demonstrates that pretreatment with this steroid also benefits embryo development and normalisation of early luteal function.
Additional keywords: 3β-hydroxysteroid dehydrogenase, corpus luteum, LH, ovary, progesterone, spontaneous ovulation.
References
Berisha, B., Pfaffl, M. W., and Schams, D. (2002). Expression of estrogen and progesterone receptors in the bovine ovary during estrous cycle and pregnancy. Endocrine 17, 207–214.| Expression of estrogen and progesterone receptors in the bovine ovary during estrous cycle and pregnancy.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xlt1SltbY%3D&md5=9bb815d39f1fe3b5c821183de49b0ba5CAS | 12108521PubMed |
Brown, J. L., Wasser, S. K., Wildt, D. E., and Graham, L. H. (1994). Comparative aspects of steroid hormone metabolism and ovarian activity in felids, measured noninvasively in feces. Biol. Reprod. 51, 776–786.
| Comparative aspects of steroid hormone metabolism and ovarian activity in felids, measured noninvasively in feces.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXlvFKhu7g%3D&md5=aa3302caf953fd883ec1dd523d0ed1b2CAS | 7819459PubMed |
Brown, J. L., Wildt, D. E., Graham, L. H., Byers, A. P., Collins, L., Barrett, S., and Howard, J. G. (1995). Natural versus chorionic gonadotropin-induced ovarian responses in the clouded leopard (Neofelis nebulosa) assessed by fecal steroid analysis. Biol. Reprod. 53, 93–102.
| Natural versus chorionic gonadotropin-induced ovarian responses in the clouded leopard (Neofelis nebulosa) assessed by fecal steroid analysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXmt12itL8%3D&md5=38b2ea4bf66a50204487c34d675a9c0bCAS | 7669862PubMed |
Comizzoli, P., Wildt, D. E., and Pukazhenthi, B. S. (2004). Effect of 1,2-propanediol versus 1,2-ethanediol on subsequent oocyte maturation, spindle integrity, fertilization, and embryo development in vitro in the domestic cat. Biol. Reprod. 71, 598–604.
| Effect of 1,2-propanediol versus 1,2-ethanediol on subsequent oocyte maturation, spindle integrity, fertilization, and embryo development in vitro in the domestic cat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXmtFWgurg%3D&md5=2158ddc21b3f936fa4e875ffe7f66811CAS | 15084479PubMed |
Comizzoli, P., Crosier, A. E., Songsasen, N., Gunther, M. S., Howard, J. G., and Wildt, D. E. (2009). Advances in reproductive science for wild carnivore conservation. Reprod. Domest. Anim. 44, 47–52.
| Advances in reproductive science for wild carnivore conservation.Crossref | GoogleScholarGoogle Scholar | 19754535PubMed |
Concannon, P. W., and Verstegen, J. (1999). Pregnancy in dogs and cats. In ‘Encyclopedia of Reproduction. Volume 3’. (Eds E. Knobil and J. D. Neill.) pp. 970–979. (Academic Press: New York, NY.)
Devoto, L., Kohen, P., Gonzalez, R. R., Castro, O., Retamales, I., Vega, M., Carvallo, P., Christenson, L. K., and Strauss, J. F. (2001). Expression of steroidogenic acute regulatory protein in the human corpus luteum throughout the luteal phase. J. Clin. Endocrinol. Metab. 86, 5633–5639.
| Expression of steroidogenic acute regulatory protein in the human corpus luteum throughout the luteal phase.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXos1Kqu7s%3D&md5=d2e940d9652bf7fa7c172182cd80a54dCAS | 11701746PubMed |
Ertzeid, G., Storeng, R., and Lyberg, T. (1993). Treatment with gonadotropins impaired implantation and fetal development in mice. J. Assist. Reprod. Genet. 10, 286–291.
| Treatment with gonadotropins impaired implantation and fetal development in mice.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK2c7ns1OrsA%3D%3D&md5=d8ca06de2c94f50d071a8f732a024cf0CAS | 8130435PubMed |
Graham, L. H., Swanson, W. F., and Brown, J. L. (2000). Chorionic gonadotropin administration in domestic cats causes an abnormal endocrine environment that disrupts oviductal embryo transport. Theriogenology 54, 1117–1131.
| Chorionic gonadotropin administration in domestic cats causes an abnormal endocrine environment that disrupts oviductal embryo transport.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXhtlCnug%3D%3D&md5=5aeb262c9ba14d256685fb812ccf81faCAS | 11131330PubMed |
Gudermuth, D. F., Newton, L., Daels, P., and Concannon, P. (1997). Incidence of spontaneous ovulation in young, group-housed cats based on serum and faecal concentrations of progesterone. J. Reprod. Fertil. Suppl. 51, 177–184.
| 1:CAS:528:DyaK2sXmvFCksrk%3D&md5=263b74331a38ebfc534d253dd910e2bfCAS | 9404283PubMed |
Herrick, J. R., Bond, J. B., Campbell, M., Levens, G., Moore, T., Benson, K., D’Agostino, J., West, G., Okeson, D. M., Coke, R., Portacio, S. C., Leiske, K., Kreider, C., Polumbo, P. J., and Swanson, W. F. (2010). Fecal endocrine profiles and ejaculate traits in black-footed cats (Felis nigripes) and sand cats (Felis margarita). Gen. Comp. Endocrinol. 165, 204–214.
| Fecal endocrine profiles and ejaculate traits in black-footed cats (Felis nigripes) and sand cats (Felis margarita).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhsVKht7zI&md5=41d56a710a1bcc71b4624c691f6db787CAS | 19576217PubMed |
Hild-Petito, S., and Fazleabas, A. T. (1997). Expression of steroid receptors and steroidogenic enzymes in the baboon (Papio anubis) corpus luteum during the menstrual cycle and early pregnancy. J. Clin. Endocrinol. Metab. 82, 955–962.
| Expression of steroid receptors and steroidogenic enzymes in the baboon (Papio anubis) corpus luteum during the menstrual cycle and early pregnancy.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXhsl2nuro%3D&md5=c91abdc522e74291cbc816681ab0012cCAS | 9062513PubMed |
Howard, J. G. (1992). Feline semen analysis and artificial insemination. In ‘Current Veterinary Therapy XI’. (Ed. R. W. Kirk.) pp. 929–938. (W. B. Saunders Co.: Philadelphia, PA.)
Howard, J. G. (1999). Assisted reproductive techniques in nondomestic carnivores. In ‘Zoo and Wild Animal Medicine: Current Therapy IV’. (Eds M. E. Fowler and R. E. Miller.) pp. 449–457. (W. B. Saunders Co.: Philadelphia, PA.)
Howard, J. G., and Wildt, D. E. (2009). Approaches and efficacy of artificial insemination in felids and mustelids. Theriogenology 71, 130–148.
| Approaches and efficacy of artificial insemination in felids and mustelids.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1cjnt1Crug%3D%3D&md5=61bc86e676a1c96429ca3ae7aa44074aCAS | 18996580PubMed |
Howard, J. G., Barone, M. A., Donoghue, A. M., and Wildt, D. E. (1992a). The effect of pre-ovulatory anaesthesia on ovulation in laparoscopically inseminated domestic cats. J. Reprod. Fertil. 96, 175–186.
| The effect of pre-ovulatory anaesthesia on ovulation in laparoscopically inseminated domestic cats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXmsFI%3D&md5=3938cd5d27a23c3d487cb0db2e19c7afCAS | 1432948PubMed |
Howard, J. G., Haskins, M. E., Patterson, D. F., Just, C. A., McAloose, D., Baker, H. J., Thrall, M. A., Gasper, P. W., and Wildt, D. E. (1992b). Assisted reproduction in feline animal models of human disease. Contemp. Top. Lab. Anim. Sci. 31, 20.
Howard, J. G., Donoghue, A. M., Johnston, L. A., and Wildt, D. E. (1993). Zona pellucida filtration of structurally abnormal spermatozoa and reduced fertilization in teratospermic cats. Biol. Reprod. 49, 131–139.
| Zona pellucida filtration of structurally abnormal spermatozoa and reduced fertilization in teratospermic cats.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK3szlslegug%3D%3D&md5=311118dac3feb04494dddd74eb21ba4aCAS | 8353179PubMed |
Kipar, A., Leutenegger, C. M., Hetzel, U., Akens, M. K., Mislin, C. N., Reinacher, M., and Lutz, H. (2001). Cytokine mRNA levels in isolated feline monocytes. Vet. Immunol. Immunopathol. 78, 305–315.
| Cytokine mRNA levels in isolated feline monocytes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXisVamtL0%3D&md5=5ebd7b18d257f31ad52def348857b0dbCAS | 11292531PubMed |
Lawler, D. F., Johnston, S. D., Hegstad, R. L., Keltner, D. G., and Owens, S. F. (1993). Ovulation without cervical stimulation in domestic cats. J. Reprod. Fertil. Suppl. 47, 57–61.
| 1:STN:280:DyaK2c%2Fks1yiuw%3D%3D&md5=7e6dc347f6c9d491474778bf303cdebaCAS | 8229985PubMed |
Lofstedt, R. M., and Patel, J. H. (1989). Evaluation of the ability of altrenogest to control the equine estrous cycle. J. Am. Vet. Med. Assoc. 194, 361–364.
| 1:STN:280:DyaL1M7jvFWrtQ%3D%3D&md5=ed1730acfa6aa1ffd79b59e0708eb185CAS | 2645256PubMed |
Martel, C., Labrie, C., Couet, J., Dupont, E., Trudel, C., Luu-The, V., Takahashi, M., Pelletier, G., and Labrie, F. (1990). Effects of human chorionic gonadotropin (hCG) and prolactin (PRL) on 3 beta-hydroxy-5-ene-steroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD) expression and activity in the rat ovary. Mol. Cell. Endocrinol. 72, R7–R13.
| Effects of human chorionic gonadotropin (hCG) and prolactin (PRL) on 3 beta-hydroxy-5-ene-steroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD) expression and activity in the rat ovary.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXls1Glt7o%3D&md5=d5f6c1c74a6b89733eca1fa9ca7d37b3CAS | 2149342PubMed |
Martel, C., Gagne, D., Couet, J., Labrie, Y., Simard, J., and Labrie, F. (1994). Rapid modulation of ovarian 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase gene expression by prolactin and human chorionic gonadotropin in the hypophysectomized rat. Mol. Cell. Endocrinol. 99, 63–71.
| Rapid modulation of ovarian 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase gene expression by prolactin and human chorionic gonadotropin in the hypophysectomized rat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXitVykurs%3D&md5=96a29a20f2375bdc5142914200b52379CAS | 8187962PubMed |
McKiernan, S. H., and Bavister, B. D. (1998). Gonadotrophin stimulation of donor females decreases post-implantation viability of cultured one-cell hamster embryos. Hum. Reprod. 13, 724–729.
| Gonadotrophin stimulation of donor females decreases post-implantation viability of cultured one-cell hamster embryos.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXivF2itLo%3D&md5=932916547dd0fc51cab27367b9e5b75dCAS | 9572442PubMed |
Molina, I., Pla, M., Vicente, J. S., Martin, A., and Romeu, A. (1991). Induction of ovulation in rabbits with pure urinary luteinizing hormone and human chorionic gonadotrophin: comparison of oocyte and embryo quality. Hum. Reprod. 6, 1449–1452.
| 1:CAS:528:DyaK38Xhs1KmurY%3D&md5=ad7d504892581fbde6e897c848f4268eCAS | 1770143PubMed |
Moreira, N., Monteiro-Filho, E. L., Moraes, W., Swanson, W. F., Graham, L. H., Pasquali, O. L., Gomes, M. L., Morais, R. N., Wildt, D. E., and Brown, J. L. (2001). Reproductive steroid hormones and ovarian activity in felids of the Leopardus genus. Zoo Biol. 20, 103–116.
| Reproductive steroid hormones and ovarian activity in felids of the Leopardus genus.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXlt1Gmu78%3D&md5=f72b17db7030ea5a32a2cd9ce7344e50CAS | 11429781PubMed |
Niswender, G. D., Juengel, J. L., Silva, P. J., Rollyson, M. K., and McIntush, E. W. (2000). Mechanisms controlling the function and life span of the corpus luteum. Physiol. Rev. 80, 1–29.
| 1:CAS:528:DC%2BD3cXmtl2jtg%3D%3D&md5=91ddbc1f315590184628584a11c89369CAS | 10617764PubMed |
Patterson, D. J., Nieman, N. M., Nelson, L. D., Nelson, C. F., Schillo, K. K., Bullock, K. D., Brophy, D. T., and Woods, B. L. (1997). Estrus synchronization with an oral progestogen prior to superovulation of postpartum beef cows. Theriogenology 48, 1025–1033.
| Estrus synchronization with an oral progestogen prior to superovulation of postpartum beef cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXmvFejurY%3D&md5=c88f00bfcb649a87b426a74e0b9ec423CAS | 16728192PubMed |
Pelican, K. M., Brown, J. L., Wildt, D. E., Ottinger, M. A., and Howard, J. G. (2005). Short term suppression of follicular recruitment and spontaneous ovulation in the cat using levonorgestrel versus a GnRH antagonist. Gen. Comp. Endocrinol. 144, 110–121.
| Short term suppression of follicular recruitment and spontaneous ovulation in the cat using levonorgestrel versus a GnRH antagonist.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtVynurjN&md5=a98cd8f05699e3badd3d8742bbda756aCAS | 15979618PubMed |
Pelican, K. M., Wildt, D. E., Pukazhenthi, B., and Howard, J. G. (2006). Ovarian control for assisted reproduction in the domestic cat and wild felids. Theriogenology 66, 37–48.
| Ovarian control for assisted reproduction in the domestic cat and wild felids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XlsFKrsL0%3D&md5=03cfbacd9c563d3c946f8aed6263df4dCAS | 16630653PubMed |
Pelican, K. M., Wildt, D. E., Ottinger, M. A., and Howard, J. G. (2008). Priming with progestin, but not GnRH antagonist, induces a consistent endocrine response to exogenous gonadotropins in induced and spontaneously ovulating cats. Domest. Anim. Endocrinol. 34, 160–175.
| Priming with progestin, but not GnRH antagonist, induces a consistent endocrine response to exogenous gonadotropins in induced and spontaneously ovulating cats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXptlaktw%3D%3D&md5=f7fbe8efb28ba659fe283493d1f346b7CAS | 17369001PubMed |
Pelican, K. M., Spindler, R. E., Pukazhenthi, B. S., Wildt, D. E., Ottinger, M. A., and Howard, J. (2010). Progestin exposure before gonadotropin stimulation improves embryo development after in vitro fertilization in the domestic cat. Biol. Reprod. 83, 558–567.
| Progestin exposure before gonadotropin stimulation improves embryo development after in vitro fertilization in the domestic cat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXht1ahtr7I&md5=9368059410d917527cd31ba64ecd2aeeCAS | 20463355PubMed |
Pescador, N., Soumano, K., Stocco, D. M., Price, C. A., and Murphy, B. D. (1996). Steroidogenic acute regulatory protein in bovine corpora lutea. Biol. Reprod. 55, 485–491.
| Steroidogenic acute regulatory protein in bovine corpora lutea.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XltlGrur0%3D&md5=4865b6cb24f4e8f9ac6b94e42c8da0b0CAS | 8828859PubMed |
Pope, C. E., Gomez, M. C., and Dresser, B. L. (2006). In vitro embryo production and embryo transfer in domestic and non-domestic cats. Theriogenology 66, 1518–1524.
| In vitro embryo production and embryo transfer in domestic and non-domestic cats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xps1Oit78%3D&md5=f3dc711106dd65d5ddfe0531ab110526CAS | 16497369PubMed |
Porter, T. E., Ellestad, L. E., Fay, A., Stewart, J. L., and Bossis, I. (2006). Identification of the chicken growth hormone-releasing hormone receptor (GHRH-R) mRNA and gene: regulation of anterior pituitary GHRH-R mRNA levels by homologous and heterologous hormones. Endocrinology 147, 2535–2543.
| Identification of the chicken growth hormone-releasing hormone receptor (GHRH-R) mRNA and gene: regulation of anterior pituitary GHRH-R mRNA levels by homologous and heterologous hormones.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XjvFOqs70%3D&md5=81778d588f96b288c17a7324ad7a600aCAS | 16469800PubMed |
Pukazhenthi, B. S., and Wildt, D. E. (2004). Which reproductive technologies are most relevant to studying, managing, and conserving wildlife? Reprod. Fertil. Dev. 16, 33–46.
| Which reproductive technologies are most relevant to studying, managing, and conserving wildlife?Crossref | GoogleScholarGoogle Scholar | 14972101PubMed |
Roth, T. L., Swanson, W. F., and Wildt, D. E. (1994). Developmental competence of domestic cat embryos fertilized in vivo versus in vitro. Biol. Reprod. 51, 441–451.
| Developmental competence of domestic cat embryos fertilized in vivo versus in vitro.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK2M7gtFylsg%3D%3D&md5=77d91c0417b1c238e3b6cc0796bb5254CAS | 7803615PubMed |
Roth, T. L., Munson, L., Swanson, W. F., and Wildt, D. E. (1995). Histological characteristics of the uterine endometrium and corpus luteum during early embryogenesis and the relationship to embryonic mortality in the domestic cat. Biol. Reprod. 53, 1012–1021.
| Histological characteristics of the uterine endometrium and corpus luteum during early embryogenesis and the relationship to embryonic mortality in the domestic cat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXoslyntbs%3D&md5=a5ed4fbc8ffa3bffdb2f1ca9c03fcfd3CAS | 8527503PubMed |
Roth, T. L., Wolfe, B. A., Long, J. A., Howard, J. G., and Wildt, D. E. (1997). Effects of equine chorionic gonadotropin, human chorionic gonadotropin, and laparoscopic artificial insemination on embryo, endocrine, and luteal characteristics in the domestic cat. Biol. Reprod. 57, 165–171.
| Effects of equine chorionic gonadotropin, human chorionic gonadotropin, and laparoscopic artificial insemination on embryo, endocrine, and luteal characteristics in the domestic cat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXktFCrtrw%3D&md5=28865c09df021e016efa7b3c7219e27aCAS | 9209095PubMed |
Santymire, R. M., Brown, J. L., Stewart, R. A., Santymire, R. C., Wildt, D. E., and Howard, J. (2011). Reproductive gonadal steroidogenic activity in the fishing cat (Prionailurus viverrinus) assessed by fecal steroid analyses. Anim. Reprod. Sci. 128, 60–72.
| Reproductive gonadal steroidogenic activity in the fishing cat (Prionailurus viverrinus) assessed by fecal steroid analyses.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsVamsr%2FE&md5=afae53578dbfcb03068edb3356a23353CAS | 21975304PubMed |
Sierralta, W. D., Kohen, P., Castro, O., Munoz, A., Strauss, J. F., and Devoto, L. (2005). Ultrastructural and biochemical evidence for the presence of mature steroidogenic acute regulatory protein (StAR) in the cytoplasm of human luteal cells. Mol. Cell. Endocrinol. 242, 103–110.
| Ultrastructural and biochemical evidence for the presence of mature steroidogenic acute regulatory protein (StAR) in the cytoplasm of human luteal cells.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtVGrsbbM&md5=d12a672470d5cec114c8bbd8263775efCAS | 16162390PubMed |
Stewart, R. A., Pelican, K. M., Brown, J. L., Wildt, D. E., Ottinger, M. A., and Howard, J. G. (2010). Oral progestin induces rapid, reversible suppression of ovarian activity in the cat. Gen. Comp. Endocrinol. 166, 409–416.
| Oral progestin induces rapid, reversible suppression of ovarian activity in the cat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXjtVCgurw%3D&md5=5c40c36887acbfb1ec9c7aeea2ff5013CAS | 20051246PubMed |
Stewart, R. A., Pelican, K. M., Crosier, A. E., Pukazhenthi, B. S., Wildt, D. E., Ottinger, M. A., and Howard, J. (2012). Oral progestin priming increases ovarian sensitivity to gonadotropin stimulation and improves luteal function in the cat. Biol. Reprod. 87, 137.
| Oral progestin priming increases ovarian sensitivity to gonadotropin stimulation and improves luteal function in the cat.Crossref | GoogleScholarGoogle Scholar | 23100619PubMed |
Stocco, C. O., Chedrese, J., and Deis, R. P. (2001). Luteal expression of cytochrome P450 side-chain cleavage, steroidogenic acute regulatory protein, 3beta-hydroxysteroid dehydrogenase, and 20alpha-hydroxysteroid dehydrogenase genes in late pregnant rats: effect of luteinizing hormone and RU486. Biol. Reprod. 65, 1114–1119.
| Luteal expression of cytochrome P450 side-chain cleavage, steroidogenic acute regulatory protein, 3beta-hydroxysteroid dehydrogenase, and 20alpha-hydroxysteroid dehydrogenase genes in late pregnant rats: effect of luteinizing hormone and RU486.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXnt1Cms78%3D&md5=3b3cfcc186c95a6791ea900469599a33CAS | 11566732PubMed |
Stocco, C., Telleria, C., and Gibori, G. (2007). The molecular control of corpus luteum formation, function, and regression. Endocr. Rev. 28, 117–149.
| The molecular control of corpus luteum formation, function, and regression.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXjtFCgsrc%3D&md5=07089d717cbab261dc61fcb3478d7d42CAS | 17077191PubMed |
Swanson, W. F. (2003). Research in nondomestic species: experiences in reproductive physiology research for conservation of endangered felids. ILAR J. 44, 307–316.
| Research in nondomestic species: experiences in reproductive physiology research for conservation of endangered felids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXntVKktr8%3D&md5=c785968dbaf7f9166e3b6abde56627beCAS | 13130161PubMed |
Swanson, W. F. (2006). Application of assisted reproduction for population management in felids: the potential and reality for conservation of small cats. Theriogenology 66, 49–58.
| Application of assisted reproduction for population management in felids: the potential and reality for conservation of small cats.Crossref | GoogleScholarGoogle Scholar | 16650889PubMed |
Swanson, W. F., Roth, T. L., and Wildt, D. E. (1994). In vivo embryogenesis, embryo migration, and embryonic mortality in the domestic cat. Biol. Reprod. 51, 452–464.
| In vivo embryogenesis, embryo migration, and embryonic mortality in the domestic cat.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK2M7gtFylsw%3D%3D&md5=65b02f9c01940a6ce5062986765a7bfcCAS | 7803616PubMed |
Swanson, W. F., Roth, T. L., Brown, J. L., and Wildt, D. E. (1995). Relationship of circulating steroid hormones, luteal luteinizing hormone receptor and progesterone concentration, and embryonic mortality during early embryogenesis in the domestic cat. Biol. Reprod. 53, 1022–1029.
| Relationship of circulating steroid hormones, luteal luteinizing hormone receptor and progesterone concentration, and embryonic mortality during early embryogenesis in the domestic cat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXoslyntbg%3D&md5=9d9c4d4576c26820c4942a401070b088CAS | 8527504PubMed |
Swanson, W. F., Graham, K., Horohov, D. W., Thompson, D. L., and Godke, R. A. (1996). Ancillary follicle and secondary corpora lutea formation following exogenous gonadotropin treatment in the domestic cat and effect of passive transfer of gonadotropin-neutralizing antisera. Theriogenology 45, 561–572.
| Ancillary follicle and secondary corpora lutea formation following exogenous gonadotropin treatment in the domestic cat and effect of passive transfer of gonadotropin-neutralizing antisera.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XisV2qsrg%3D&md5=2b48edfa1bb13548a796bf134e177cc1CAS | 16727819PubMed |
Swanson, W. F., Wolfe, B. A., Brown, J. L., Martin-Jimenez, T., Riviere, J. E., Roth, T. L., and Wildt, D. E. (1997). Pharmacokinetics and ovarian-stimulatory effects of equine and human chorionic gonadotropins administered singly and in combination in the domestic cat. Biol. Reprod. 57, 295–302.
| Pharmacokinetics and ovarian-stimulatory effects of equine and human chorionic gonadotropins administered singly and in combination in the domestic cat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXkslWht7w%3D&md5=f7a01b77a1c50f1ad375fb3bc1139a34CAS | 9241042PubMed |
Swanson, W., McRae, M., Bond, J., Melniczek, J., and Haskins, M. (2000). Homologous and heterologous mucopolysaccharidosis kittens produced by transfer of frozen-thawed IVF embryos. Biol. Reprod. 62, 319–320.
Thiangtum, K., Swanson, W. F., Howard, J., Tunwattana, W., Tongthainan, D., Wichasilpa, W., Patumrattanathan, P., and Pinyopoommintr, T. (2006). Assessment of basic seminal characteristics, sperm cryopreservation and heterologous in vitro fertilisation in the fishing cat (Prionailurus viverrinus). Reprod. Fertil. Dev. 18, 373–382.
| Assessment of basic seminal characteristics, sperm cryopreservation and heterologous in vitro fertilisation in the fishing cat (Prionailurus viverrinus).Crossref | GoogleScholarGoogle Scholar | 16554013PubMed |
Tsutsui, T., and Stabenfeldt, G. H. (1993). Biology of ovarian cycles, pregnancy and pseudopregnancy in the domestic cat. J. Reprod. Fertil. Suppl. 47, 29–35.
| 1:STN:280:DyaK2c%2Fks1Oksg%3D%3D&md5=cead419c88f27601e0c5257be82bde72CAS | 8229938PubMed |
Verstegen, J. P., Onclin, K., Silva, L. D., Wouters-Ballman, P., Delahaut, P., and Ectors, F. (1993). Regulation of progesterone during pregnancy in the cat: studies on the roles of corpora lutea, placenta and prolactin secretion. J. Reprod. Fertil. Suppl. 47, 165–173.
| 1:STN:280:DyaK2c%2Fks1Omtw%3D%3D&md5=0f9776c4d68df76d886725e46d08dde7CAS | 8229923PubMed |
Wildt, D. E., Comizzoli, P., Pukazhenthi, B., and Songsasen, N. (2010a). Lessons from biodiversity: the value of nontraditional species to advance reproductive science, conservation, and human health. Mol. Reprod. Dev. 77, 397–409.
| Lessons from biodiversity: the value of nontraditional species to advance reproductive science, conservation, and human health.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXjvFKqtr0%3D&md5=abaaf367938523496a27c03201828cf6CAS | 19967718PubMed |
Wildt, D. E., Swanson, W. F., Brown, J. L., Sliwa, A., and Vargas, A. (2010b). Felids ex situ for managed programmes, research, and species recovery. In ‘Biology and Conservation of Wild Felids’. (Eds D. W. MacDonald and A. Loveridge.) pp. 217–235. (Oxford University Press: Oxford, UK.)
Wood, C. M., Kornegay, E. T., and Shipley, C. F. (1992). Efficacy of altrenogest in synchronizing estrus in two swine breeding programs and effects on subsequent reproductive performance of sows. J. Anim. Sci. 70, 1357–1364.
| 1:CAS:528:DyaK38XltVCntrY%3D&md5=6a7e12466ed7718daa2be54386127ea5CAS | 1526904PubMed |
