Recipient of the 2022 IETS Pioneer Award: Dr Carol Keefer

References

Baldassarre, H, Wang, B, Kafidi, N, Keefer, C, Lazaris, A, and Karatzas, CN (2002). Advances in the production and propagation of transgenic goats using laparoscopic ovum pick-up and in vitro embryo production technologies. Theriogenology 57, 275–284.
| Advances in the production and propagation of transgenic goats using laparoscopic ovum pick-up and in vitro embryo production technologies.Crossref | GoogleScholarGoogle Scholar |

Brown, ME, Converse, SJ, Chandler, JN, Shafer, C, Brown, JL, Keefer, CL, and Songsasen, N (2016). Female gonadal hormones and reproductive behaviors as key determinants of successful reproductive output of breeding whooping cranes (Grus americana). General and Comparative Endocrinology 230–231, 158–165.
| Female gonadal hormones and reproductive behaviors as key determinants of successful reproductive output of breeding whooping cranes (Grus americana).Crossref | GoogleScholarGoogle Scholar |

Brown, ME, Converse, SJ, Chandler, JN, Crosier, AL, Lynch, W, Wildt, DE, Keefer, CL, and Songsasen, N (2017). Time within reproductive season, but not age or inbreeding coefficient, affects seminal and sperm quality in the whooping crane (Grus americana). Reproduction, Fertility and Development 29, 294–306.
| Time within reproductive season, but not age or inbreeding coefficient, affects seminal and sperm quality in the whooping crane (Grus americana).Crossref | GoogleScholarGoogle Scholar |

Brown, ME, Singh, RP, Pukazhenthi, B, Keefer, CL, and Songsasen, N (2018). Cryopreservation effects on sperm function and fertility in two threatened crane species. Cryobiology 82, 148–154.
| Cryopreservation effects on sperm function and fertility in two threatened crane species.Crossref | GoogleScholarGoogle Scholar |

Brown, ME, Keefer, CL, and Songsasen, N (2019). Factors affecting captive whooping crane egg fertility: a retrospective analysis. The Journal of Wildlife Management 83, 1377–1386.
| Factors affecting captive whooping crane egg fertility: a retrospective analysis.Crossref | GoogleScholarGoogle Scholar |

Chung, J, Clifford, R, Sriram, G, and Keefer, C (2019). 68 Flux analysis of aerobic glycolysis in bovine blastocysts and CT1 cells. Reproduction, Fertility and Development 31, 159.
| 68 Flux analysis of aerobic glycolysis in bovine blastocysts and CT1 cells.Crossref | GoogleScholarGoogle Scholar |

Collins, CW, Monfort, SL, Vick, MM, Wolfe, BA, Weiss, RB, Keefer, CL, and Songsasen, N (2014). Oral and injectable synthetic progestagens effectively manipulate the estrous cycle in the Przewalski’s horse (Equus ferus przewalskii). Animal Reproduction Science 148, 42–52.
| Oral and injectable synthetic progestagens effectively manipulate the estrous cycle in the Przewalski’s horse (Equus ferus przewalskii).Crossref | GoogleScholarGoogle Scholar |

Fujihara, M, Comizzoli, P, Keefer, CL, Wildt, DE, and Songsasen, N (2014). Epidermal growth factor (EGF) sustains in vitro primordial follicle viability by enhancing stromal cell proliferation via MAPK and PI3K pathways in the prepubertal, but not adult cat ovary. Biology of Reproduction 90, 86.
| Epidermal growth factor (EGF) sustains in vitro primordial follicle viability by enhancing stromal cell proliferation via MAPK and PI3K pathways in the prepubertal, but not adult cat ovary.Crossref | GoogleScholarGoogle Scholar |

Gauthier, M, Pierson, J, Drolet, M, Bhatia, B, Baldassarre, H, and Keefer, CL (2001). Sexual maturation and fertility of male Nigerian dwarf goat (Capra hircus) clones produced by somatic cell nuclear transfer. Cloning and Stem Cells 3, 151–155.
| Sexual maturation and fertility of male Nigerian dwarf goat (Capra hircus) clones produced by somatic cell nuclear transfer.Crossref | GoogleScholarGoogle Scholar |

He, S, Pant, D, Schiffmacher, A, Bischoff, S, Melican, D, Gavin, W, and Keefer, C (2006). Developmental expression of pluripotency determining factors in caprine embryos: novel pattern of NANOG protein localization in the nucleolus. Molecular Reproduction and Development 73, 1512–1522.
| Developmental expression of pluripotency determining factors in caprine embryos: novel pattern of NANOG protein localization in the nucleolus.Crossref | GoogleScholarGoogle Scholar |

He, S, Pant, D, Schiffmacher, A, Meece, A, and Keefer, CL (2008). Lymphoid enhancer factor 1-mediated Wnt signaling promotes the initiation of trophoblast lineage differentiation in mouse embryonic stem cells. Stem Cells 26, 842–849.
| Lymphoid enhancer factor 1-mediated Wnt signaling promotes the initiation of trophoblast lineage differentiation in mouse embryonic stem cells.Crossref | GoogleScholarGoogle Scholar |

Keefer, CL (1989). Fertilization by sperm injection in the rabbit. Gamete Research 22, 59–69.
| Fertilization by sperm injection in the rabbit.Crossref | GoogleScholarGoogle Scholar |

Keefer, CL, and Desai, JP (2011). Mechanical phenotyping of stem cells. Theriogenology 75, 1426–1430.
| Mechanical phenotyping of stem cells.Crossref | GoogleScholarGoogle Scholar |

Keefer, CL, and Schuetz, AW (1982). Spontaneous activation of ovulated rat oocytes during in vitro culture. Journal of Experimental Zoology 224, 371–377.
| Spontaneous activation of ovulated rat oocytes during in vitro culture.Crossref | GoogleScholarGoogle Scholar |

Keefer, CL, and Tasca, RJ (1984). Modulation of amino acid transport in preimplantation mouse embryos by low concentrations of non-ionic and zwitterionic detergents. Journal of Reproduction and Fertility 70, 399–407.
| Modulation of amino acid transport in preimplantation mouse embryos by low concentrations of non-ionic and zwitterionic detergents.Crossref | GoogleScholarGoogle Scholar |

Keefer, CL, Bennett, KA, and Brackett, BG (1985). In vitro fertilization in the rabbit after delayed ovum recovery. Biology of Reproduction 33, 388–392.
| In vitro fertilization in the rabbit after delayed ovum recovery.Crossref | GoogleScholarGoogle Scholar |

Keefer, CL, Fayrer-Hosken, RA, Brown, LM, and Brackett, BG (1988). Culture of in vitro fertilized rabbit ova. Gamete Research 20, 431–436.
| Culture of in vitro fertilized rabbit ova.Crossref | GoogleScholarGoogle Scholar |

Keefer, CL, Stice, SL, and Dobrinsky, J (1993). Effect of FSH and LH during bovine in vitro maturation on development following in vitro fertilization and nuclear transfer. Molecular Reproduction and Development 36, 469–474.
| Effect of FSH and LH during bovine in vitro maturation on development following in vitro fertilization and nuclear transfer.Crossref | GoogleScholarGoogle Scholar |

Keefer, CL, Stice, SL, and Matthews, DL (1994). Bovine inner cell mass (ICM) cells as donor nuclei in the production of nuclear transfer embryos. Biology of Reproduction 50, 935–939.
| Bovine inner cell mass (ICM) cells as donor nuclei in the production of nuclear transfer embryos.Crossref | GoogleScholarGoogle Scholar |

Keefer, CL, Baldassarre, H, Keyston, R, Wang, B, Bhatia, B, Bilodeau, AS, Zhou, JF, Leduc, M, Downey, BR, Lazaris, A, and Karatzas, CN (2001). Generation of dwarf goat (Capra hircus) clones following nuclear transfer with transfected and nontransfected fetal fibroblasts and in vitro-matured oocytes. Biology of Reproduction 64, 849–856.
| Generation of dwarf goat (Capra hircus) clones following nuclear transfer with transfected and nontransfected fetal fibroblasts and in vitro-matured oocytes.Crossref | GoogleScholarGoogle Scholar |

Keefer, CL, Keyston, R, Lazaris, A, Bhatia, B, Begin, I, Bilodeau, AS, Zhou, FJ, Kafidi, N, Wang, B, Baldassarre, H, and Karatzas, CN (2002). Production of cloned goats after nuclear transfer using adult somatic cells. Biology of Reproduction 66, 199–203.
| Production of cloned goats after nuclear transfer using adult somatic cells.Crossref | GoogleScholarGoogle Scholar |

Keefer, CL, Pant, D, Blomberg, L, and Talbot, NC (2007). Challenges and prospects for the establishment of embryonic stem cell lines of domesticated ungulates. Animal Reproduction Science 98, 147–168.
| Challenges and prospects for the establishment of embryonic stem cell lines of domesticated ungulates.Crossref | GoogleScholarGoogle Scholar |

Ladjal, H, Hanus, JL, Pillarisetti, A, Keefer, C, Ferreira, A, and Desai, JP (2012). Reality-based real-time cell indentation stimulator. IEEE/ASME Transactions on Mechatronics 17, 239–250.
| Reality-based real-time cell indentation stimulator.Crossref | GoogleScholarGoogle Scholar |

Nestle, E, Graves-Herring, J, Keefer, C, and Comizzoli, P (2012). Source of protein supplementation during in vitro culture does not affect the quality of resulting blastocysts in the domestic cat. Reproduction in Domestic Animals 47, 152–155.
| Source of protein supplementation during in vitro culture does not affect the quality of resulting blastocysts in the domestic cat.Crossref | GoogleScholarGoogle Scholar |

Pant, D, and Keefer, CL (2009). Expression of pluripotency-related genes during bovine inner cell mass explant culture. Cloning and Stem Cells 11, 355–365.
| Expression of pluripotency-related genes during bovine inner cell mass explant culture.Crossref | GoogleScholarGoogle Scholar |

Perreault, SD, Barbee, RR, Elstein, KH, Zucker, RM, and Keefer, CL (1988). Interspecies differences in the stability of mammalian sperm nuclei assessed in vivo by sperm microinjection and in vitro by flow cytometry. Biology of Reproduction 39, 157–167.
| Interspecies differences in the stability of mammalian sperm nuclei assessed in vivo by sperm microinjection and in vitro by flow cytometry.Crossref | GoogleScholarGoogle Scholar |

Pillarisetti, A, Desai, JP, Ladjal, H, Schiffmacher, A, Ferreira, A, and Keefer, CL (2011). Mechanical phenotyping of mouse embryonic stem cells: increase in stiffness with differentiation. Cellular Reprogramming 13, 371–380.
| Mechanical phenotyping of mouse embryonic stem cells: increase in stiffness with differentiation.Crossref | GoogleScholarGoogle Scholar |

Stice, SL, and Keefer, CL (1993). Multiple generational bovine embryo cloning. Biology of Reproduction 48, 715–719.
| Multiple generational bovine embryo cloning.Crossref | GoogleScholarGoogle Scholar |

Stice, SL, Keefer, CL, and Matthews, L (1994). Bovine nuclear transfer embryos: oocyte activation prior to blastomere fusion. Molecular Reproduction and Development 38, 61–68.
| Bovine nuclear transfer embryos: oocyte activation prior to blastomere fusion.Crossref | GoogleScholarGoogle Scholar |

Stice, SL, Strelchenko, NS, Keefer, CL, and Matthews, L (1996). Pluripotent bovine embryonic cell lines direct embryonic development following nuclear transfer. Biology of Reproduction 54, 100–110.
| Pluripotent bovine embryonic cell lines direct embryonic development following nuclear transfer.Crossref | GoogleScholarGoogle Scholar |

Thuwanut, P, Comizzoli, P, Wildt, DE, Keefer, CL, and Songsasen, N (2017). Stem cell factor promotes in vitro ovarian follicle development in the domestic cat by upregulating c-kit mRNA expression and stimulating the phosphatidylinositol 3-kinase/AKT pathway. Reproduction, Fertility and Development 29, 1356–1368.
| Stem cell factor promotes in vitro ovarian follicle development in the domestic cat by upregulating c-kit mRNA expression and stimulating the phosphatidylinositol 3-kinase/AKT pathway.Crossref | GoogleScholarGoogle Scholar |

Vansandt, LM, Pukazhenthi, BS, and Keefer, CL (2012). Molecular markers of spermatogonial stem cells in the domestic cat. Reproduction in Domestic Animals 47, 256–260.
| Molecular markers of spermatogonial stem cells in the domestic cat.Crossref | GoogleScholarGoogle Scholar |

Vansandt, LM, Livesay, JL, Dickson, MJ, Li, L, Pukazhenthi, BS, and Keefer, CL (2016). Conservation of spermatogonial stem cell marker expression in undifferentiated felid spermatogonia. Theriogenology 86, 1022–1035.e3.
| Conservation of spermatogonial stem cell marker expression in undifferentiated felid spermatogonia.Crossref | GoogleScholarGoogle Scholar |

Weiner, HS, Crosier, AE, and Keefer, CL (2019). Analysis of metabolic flux in felid spermatozoa using metabolomics and 13C-based fluxomics. Biology of Reproduction 100, 1261–1274.
| Analysis of metabolic flux in felid spermatozoa using metabolomics and ¹³C-based fluxomics.Crossref | GoogleScholarGoogle Scholar |

Zhou, R, Comizzoli, P, and Keefer, CL (2019a). Endogenous pluripotent factor expression after reprogramming cat fetal fibroblasts using inducible transcription factors. Molecular Reproduction and Development 86, 1671–1681.
| Endogenous pluripotent factor expression after reprogramming cat fetal fibroblasts using inducible transcription factors.Crossref | GoogleScholarGoogle Scholar |

Zhou, R, Wildt, DE, Keefer, CL, and Comizzoli, P (2019b). Combinations of growth factors regulating LIF/STAT3, WNT, and FGF2 pathways sustain pluripotency-related proteins in cat embryonic cells. Stem Cells and Development 28, 329–340.
| Combinations of growth factors regulating LIF/STAT3, WNT, and FGF2 pathways sustain pluripotency-related proteins in cat embryonic cells.Crossref | GoogleScholarGoogle Scholar |