167 BLACK TEA DOES NOT INTERFERE IN THE NUMBER OF CORPORA LUTEA IN SUPEROVULATED RATS
E. S. C. Kikuchi A , C. Castilho A , P. C. Papa B , C. B. Laposy A , R. M. B. Nogueira A , J. Giometti A , L. G. Machado A , R. Giuffrida A , D. P. Vieira A and I. C. Giometti A BA University of Western São Paulo, Veterinary Medicine, Presidente Prudente, Brazil;
B University of São Paulo, Department of Physiology and Biophysics, São Paulo, Brazil
Reproduction, Fertility and Development 25(1) 232-232 https://doi.org/10.1071/RDv25n1Ab167
Published: 4 December 2012
Abstract
The black tea originating from the Camellia sinensis plant is considered a healthy drink because it is associated at prevention of cancer, cardiovascular disease, and osteoporosis. It has been also stated that its catechins have antioxidative, antibacterial, and antitumoral activity. Despite many potential benefits of black tea consumption, the catechins present in black tea can significantly inhibit proliferation, steroidogenesis, and vascular endothelial growth factor production by swine granulosa cells. Based on these statements, the aim of this work was to verify the influence of black tea in the ovulation rate and the ovary weight in superovulated rats. This study was approved by the ethics committee of the university. Wistar rats were distributed in two groups: treated group (BT): 30 rats drinking only black tea (2.5%) ad libitum; control group (CG): 30 rats drinking water ad libitum. The experiment lasted for 3 months. Ten animals of each group were superovulated monthly with 150 UI kg–1 of eCG (Folligon®, Intervet Schering-Plough) and 150 UI kg–1 of hCG (Vetecor®, Hertape Calier) and sacrificed 48 h later. Ovaries were collected and weighted, and the corpora lutea were dissected and counted. The obtained data were submitted to the unpaired t-test with Welch’s correction, and the effect of time was assessed by ANOVA (P < 0.05). It was observed that the animal of both groups responded to the hormonal treatment because superovulation was noticed for the number of corpora lutea in both groups. The mean and standard deviation values for ovary weight (g) and corpora lutea number in both groups were as follows: first month (CG = 0.30 ± 0.10, 61.10 ± 10.94 and BT = 0.30 ± 0.07, 55.00 ± 23.03); second month (CG = 0.19 ± 0.06, 64.90 ± 27.77 and BT = 0.17 ± 0.05, 50.60 ± 19.35); and third month (CG = 0.21 ± 0.05, 62.30 ± 20.17 and BT = 0.19 ± 0.07, 48.6 ± 10.59), respectively. No difference was observed between the groups in the time points. The chronic consumption of black tea for 3 months does not interfere with ovulation aornd weight of the ovaries in superovulated rats.
Financial support was provided by FAPESP 2010/20583-2 and 2010/20274-0.
