345 INFLUENCE OF DIFFERENT MATURATION MEDIA ON IN VITRO SWINE EMBRYO DEVELOPMENT

Abstract

The aim of this work was to study the influence of maturation media NCSU23, Whitten, and TCM199 on in vitro development of swine embryos. Oocytes from slaughtered gilt ovaries were in vitro-matured (IVM) with NCSU23 (n = 148), Whitten (n = 151), and TCM199 (n = 170) media for 44 h. After IVM, all oocytes were fertilized in mTBM medium for 6 h and cultured in NCSU23 with 0.4% BSA for 5 days. After Day 5, all embryos were cultured in NCSU23 supplemented with 20% FCS for 4 days. Cleavage, blastocyst, and hatching rates were evaluated. Cleavage rates were 29% for NCSU23, 35.8% for Whitten, and 42.3% for TCM199 maturation media, with no significant difference among media (P > 0.05). Blastocyst rates were similar (P > 0.05), 18.9%, 21.8%, and 20%, respectively, for NCSU23, Whitten and TCM199 maturation media. Hatching rates were 16.1%, 34.3%, and 32.3%, respectively, for NCSU23, Whitten, and TCM199 maturation media, with no significant differences (P > 0.05). In conclusion, all three maturation media supported IVF, allowing embryo development from cleavage until blastocyst hatching.