315 THE REDUCTION OF MATURATIONAL COMPETENCE BY STREPTOMYCIN DURING IN VITRO MATURATION OF GOAT FOLLICULAR OOCYTES
J.K. Kang A , J.H. Yang A , K. Naruse A , C.S. Park A , K.S. Min B and D.I. Jin AA Research Center for Transgenic Cloned Pigs, Chungnam National University, Daijon, South Korea. email: dijin@cnu.ac.kr;
B Graduate School of Bio & Information Technology, Hankyong National University, Ansung, South Korea.
Reproduction, Fertility and Development 16(2) 277-277 https://doi.org/10.1071/RDv16n1Ab315
Submitted: 1 August 2003 Accepted: 1 October 2003 Published: 2 January 2004
Abstract
Antibiotics are commonly added to mammalian oocyte maturation media, but their effects on oocytes maturation have not been examined thoroughly. Goat follicular oocytes were used to investigate whether penicillin, streptomycin or gentamycin affect maturational competence of oocytes and subsequent parthenogenetic activation potential in vitro. Cumulus-oocyte complexes collected from a local abattoir were matured for 24 h in five treatments, and matured oocytes were cultured for 48 h in five treatments after parthenogenetic activation by treatment with ionomycin, followed by immediate exposure to 6-diethlaminopurine; (1) Control: TCM-199 medium with no antibiotics, (2) TCM-199 with 100 IU/mL−1 penicillin (P-4687, Sigma, St. Louis, MO, USA), (3) TCM-199 with 50 μg mL−1 streptomycin (S-1277, Sigma), (4) TCM-199 with 50 μg mL−1 gentamycin (G-1264, Sigma) and (5) TCM-199 with both 100 IU mL−1 penicillin and 50 μg mL−1 streptomycin. Maturation rates at 24 h post-in vitro maturation and parthenogenetic cleavage development at 48 h post-activation were evaluated. Data were analyzed by ANOVA and Student’s t-test. Penicillin and gentamicin treatment groups did not affect maturation rates and percentages of cleavage to 2–4 cell stage at 48 h post-chemical oocyte activation. However, when streptomycin was present in the maturation medium, the percentages of matured oocytes at 24 h post-in vitro maturation of immature goat oocytes were significantly lower than those from the other groups. However, among the five treatments, there was no significant difference in cleavage rates of matured oocytes at 48 h post-activation (Table 1). Therefore, streptomycin did interfere with the maturation of immature goat oocytes, but did not affect the subsequent development of matured goat oocytes. The mechanism by which streptomycin affects the maturation of goat follicular oocytes needs to be investigated further. We conclude that streptomycin in oocyte maturation medium can be detrimental during in vitro maturation of goat follicular oocytes.
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