129 Polar body extrusion following exposure of pig oocytes to different in vitro maturation media
M. L. Mphaphathi A E , K. M. Honneysett B , T. T. Maduwa A C , K. G. Pohler D and T. L. Nedambale CA Animal Production, Agricultural Research Council, Irene, South Africa;
B Department of Animal and Wildlife Sciences, University of Pretoria, Pretoria, South Africa;
C Department of Animal Sciences, Faculty of Science, Tshwane University of Technology, Pretoria, South Africa;
D Department of Animal Science, Texas A&M University, College Station, TX, USA;
E Department of Animal, Wildlife and Grassland Sciences, University of the Free State, Bloemfontein, South Africa
Reproduction, Fertility and Development 33(2) 173-173 https://doi.org/10.1071/RDv33n2Ab129
Published: 8 January 2021
Abstract
The in vitro maturation (IVM) rate of pig oocytes is a critical step for subsequent in vitro embryo production. The objective of the present study was to compare different IVM media (NCSU 23, NCSU 37, TCM-199, pFF, BO-IVM commercial, and Bovine IVM) on polar body extrusion in pig oocytes following 44 h of maturation duration. Ovaries from sows were collected from the local slaughterhouse and transported in warmed (38°C) saline to the laboratory, within 2 h following slaughter. The slicing method was used to retrieve oocytes from the ovaries. Collected oocytes (n = 353) were selected and washed three times in TCM-199 + 10% fetal bovine serum. The oocytes were then subjected to IVM as per treatment groups for 44 h. Following maturation period, oocytes were removed from each maturation medium and denuded of granulosa cells by vortexing. The oocytes polar body extrusion was evaluated with the aid of OosightTM Imaging Sytem connected to an inverted research microscope. Treatment means were compared using Fisher’s protected least significant difference t-test. Polar body extrusion was 30.0, 18.5, 32.8, 22.1, 32.1, and 27.0% for BO IVM commercial, Bovine IVM, NCSU 23, NCSU 37, TCM-199, and pFF media, respectively (P > 0.05). The NCSU 23 and TCM-199 had numerically higher percentages of oocytes with extruded polar body compared with other treatments groups (P > 0.05). The lowest (18.5%) polar body extrusion was recorded in Bovine IVM medium. In conclusion, all the IVM media tested can be used during IVM of pig oocytes, with NCSU 23 and TCM-199 having numerically higher polar body extrusion percentages.
