121 Comparing Two Superstimulation Protocols Prior to Ovum Pick-Up in Dromedary Camel
T. Ararooti A , A. Niasari-Naslaji A B and K. Razavi BA Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran;
B Camel Advanced Reproductive Technologies Centre, Government of Dubai, Dubai, United Arab Emirates
Reproduction, Fertility and Development 30(1) 200-200 https://doi.org/10.1071/RDv30n1Ab121
Published: 4 December 2017
Abstract
The objective of this study was to investigate different superstimulation protocol before oum pick-up (OPU) to increase the number of follicles suitable for puncture in dromedary camel. Follicular waves were synchronized using two intramuscular administrations of gonadotropin-releasing hormone (GnRH) analogue (Alarelin, 25 µg, Vetaroline®, Aburaihan, Iran) 12 to 15 days apart. Two days after the second GnRH (Day 0 of experiment), donors were assigned into 2 groups (n = 6 in each group) receiving different amounts of eCG (Pregnecol®, Bioniche, Canada; intramuscular) and FSH (Folltropin-V®, Bioniche, Canada; intramuscular). Groups 1 and 2 received the respective amounts of eCG (3000 and 2000 IU) and FSH (200 and 160 mg). The eCG was administered as a single dose at the start of superstimulation and FSH was delivered twice daily in decreasing doses (group 1: 40, 30, 20, 10 mg; group 2: 30, 20, 20, 10 mg) between Days 0 and 3. On Day 4 of superstimulation, camel received 2 doses of prostaglandin F2α analogue (25 mg of dinoprost tromethamine; Lutalyse®, Pfizer, Belgium; intramuscular), 12 h apart and were examined by ultrasonography in the morning to record ovarian follicles (≥4 mm in diameter). Data were analysed using GLM procedure followed by Tukey in SAS (SAS Institute Inc., Cary, NC, USA). Data were presented as mean ± SEM. On Day 4 of superstimulation, the average follicle diameter was similar in group 1 (6.4 ± 0.16 mm) and group 2 (7.4 ± 0.33 mm; P > 0.05). The total number of follicles were different between group 1 (15.5 ± 1.75) and group 2 (4.7 ± 0.71; P < 0.001). Total number of follicles ≤6 was significantly greater in group 1 (6.2 ± 0.95) than in group 2 (1.2 ± 0.60; P < 0.05). Total number of follicles >6 mm in group 1 (9.3 ± 1.20) was greater than in group 2 (3.5 ± 0.76; P < 0.01). In conclusion, superstimulation with eCG (3000 IU) and FSH (200 mg) could provide a relatively good number of follicles for aspiration in OPU program of dromedary camel.
