206 BIOPSY ASSESSMENT OF BOVINE OVARIAN TISSUE XENOGRAFTED UNDER RENAL CAPSULE OF SEVERE COMBINED IMMUNODEFICIENCY MICE (PRELIMINARY RESULTS)
M. B. Bezerra A , M. F. Macedo A , M. C. Matos A , G. Z. Mingoti B , J. M. Garcia A , W. R. R. Vicente A and M. R. Pacheco AA Universidade Estadual Paulista, Jaboticabal, São Paulo, Brazil;
B Universidade Estadual Paulista, Ara¸atuba, São Paulo, Brazil;
C Universidade Federal Rural do Semi�Arido, Mossoro, Rio Grande do Norte, Brazil
Reproduction, Fertility and Development 22(1) 260-260 https://doi.org/10.1071/RDv22n1Ab206
Published: 8 December 2009
Abstract
Xenografting of ovarian tissue has been considered to be a promising technique for conservation of a wide range of endangered breeds and wild animals. However, this technique requires immunosuppressed animals as recipients. Reduction of their number is desirable according to ethical principles of animal experimentation and to the costs associated with the acquisition and maintenance of severe combined immunodeficiency (SCID) or nude mouse strains. This work aims to evaluate 4 grafts per animal biopsied under the left renal capsule of SCID mice on Days 18, 30, 45, and 60 post-grafting. Thus, 8 SCID male mice that were previously orchiectomized and xenografted with 4 pieces of bovine ovarian tissue under the renal capsule were anaesthetized intraperitoneally with 10 mL kg-1 of body weight of tribromoethanol (2.5%), shaved, and antiseptically submitted to a left sublumbar incision of the skin and muscle until the identification of the left kidney containing grafts and its exposure for a biopsy procedure. One graft per animal was gently punched with a 2-mm-diameter dermatologic needle, collected with iris forceps, and immediately immersed in Bouin’s solution for histology and staining with Masson’s trichrome. Our preliminary results indicated that animals submitted to xenografting of ovarian tissue and animals that reached the first observation day (2 animals from 8) presented small subcapsular bleeding during the biopsy procedure that was easily controlled by hemostasis with local compression using sterile gauzes. Biopsied grafts presented adequate dimensions for evaluation. Ovarian tissues interacted with the surrounding renal parenchyma, were well vascularized, and contained preantral follicles. The biopsy of 1 of 4 grafts in 2 mice is part of an ongoing study. However, the first attempts on Day 18 seemed encouraging.
This work was supported by a grant from the National Counsel of Technological and Scientific Development (CNPq-Brazil) and financial support of State of São Paulo Research Foundation. (FAPESP-07/04507-1)
