Cytoplasmic maturation of the marsupial oocyte during the periovulatory period

Abstract

During the period immediately before ovulation, the oocytes of most eutherian and marsupial mammals complete the first meiotic maturation division and extrude the first polar body. In marsupials, this phase of nuclear maturation is accompanied by an increase in size of the egg and maturation of cytoplasmic components. Oocytes from at least four marsupial species, Trichosurus vulpecula, Macropus eugenii, Bettongia penicillata and Monodelphis domestica, continue to grow after formation of the follicular antrum and, although the rate of growth slows in larger follicles, it continues into the period immediately before ovulation. The basis of this growth is unknown but may include accumulation of fluid and/or yolk-like material. Maturational changes within the cytoplasm of the oocyte also occur during the periovulatory period, including the accumulation of cortical granules. Differences in the structure of the zona pellucida are also evident between follicular and ovulated eggs; these differences are suggestive of compression of the zona pellucida, but may involve the addition of extra material. These findings suggest that the marsupial oocyte may not achieve complete cytoplasmic maturity until after ovulation; however, their relevance to fertilization and embryonic development require further investigation. Like those of eutherian mammals, marsupial oocytes undergo spontaneous nuclear maturation once removed from the follicular environment, suggesting a basically similar control system. It is not known whether the preovulatory cytoplasmic changes seen in marsupial oocytes matured in vivo also occur during maturation in vitro.