Effect of active immunization against the amino-terminal peptide (alpha N) of the alpha 43 kDa subunit of inhibin (alpha 43) on fertility of ewes

Abstract

Immunization against the amino-terminal peptide (alpha N) of the alpha 43 subunit of inhibin was shown previously to reduce fertility in ewes. The aim of this study was to examine the effects of active immunization of ewes against alpha N on egg recovery and fertilization rates. Ewes were immunized against alpha N immunogen, and were given 800 I.U. of pregnant mare's serum gonadotrophin at the end of treatment with intravaginal progesterone to synchronize the oestrous cycles. Control ewes received adjuvant only. The ewes were run with fertile rams, and 4 days after withdrawal of the progesterone device the oviducts were flushed to recover eggs and luteal structures on the ovaries were recorded. Eggs were recovered from 17/19 (90%) control ewes compared with 4/16 treated ewes (25%) (P < 0.01), and the egg recovery rates were 76% (45/59) and 17% (7/42) respectively (P < 0.001). The mean number of corpora lutea (CL) per ewe were similar (3.1 +/- 1.4 v. 2.6 +/- 1.0) but several CL in the treated ewes did not appear to have ruptured, and 2 treated ewes had cystic follicles and no CL. There were no apparent differences in either the fertilization rates or the stages of development of fertilized eggs between treated and control ewes. Antibody binding levels in follicular fluid were approximately half those found in peripheral plasma. It is concluded that immunization of ewes against alpha N leads to lowered fertility by suppressing ovulation, implicating alpha N in the normal ovulatory process.