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RESEARCH ARTICLE
Contents Vol 36(4)

Lycopene inhibits apoptosis of mouse spermatocytes in varicocele via miR-23a/b-induced downregulation of PROK2

Hongqiang Wang A , Baojuan Zhu B , Tao Jing A , Lei Yu A , Kaishu Zhang A , Yujie Liu A and Hanshu Wang https://orcid.org/0000-0002-9996-5114 A *
+ Author Affiliations
- Author Affiliations

A Department of Andrology, The Affiliated Hospital of Qingdao University, No. 16 Jiangsu Road, Shinan District, Qingdao, Shandong Province 266000, China.

B Department of Hemodialysis Room, Nephrology, The Affiliated Hospital of Qingdao University, No. 16 Jiangsu Road, Shinan District, Qingdao, Shandong Province 266000, China.

* Correspondence to: wanghanshu@qdu.edu.cn

Handling Editor: Jessica Dunleavy

Reproduction, Fertility and Development 36, RD23136 https://doi.org/10.1071/RD23136
Submitted: 30 July 2023  Accepted: 15 January 2024  Published online: 2 February 2024

© 2024 The Author(s) (or their employer(s)). Published by CSIRO Publishing

Abstract

Context

The varicocele is the leading cause of male infertility and can impair sperm quality and testicular function through various mechanisms. In our previous study, we found that lycopene could attenuate hypoxia-induced testicular injury.

Aims

To illustrate the detailed mechanism of lycopene on spermatocytes.

Methods

The effect of lycopene on GC-2 cells under hypoxia were detected by flow cytometry and western blot assay. miR-seq was used to determine miRNA expression in varicocele rat model testes. The function of miR-23a/b were determined by flow cytometry and western blot assay.

Key results

We demonstrate that lycopene could alleviate hypoxia-induced GC-2 cell apoptosis and could elevate miR-23a/b expression of the hypoxia model in vivo and in vitro. The miR-23a and -23b mimics could reduce the hypoxia-induced GC-2 cell apoptosis. Both miR-23a and -23b could directly bind with prokineticin 2 (PROK2) mRNA and downregulate its expression.

Conclusions

Lycopene could attenuate hypoxia-induced spermatocyte injury through the miR-23a/b–PROK2 pathway.

Implications

Lycopene may be an effective treatment for varicocele to improve testicular impairment.

Keywords: apoptosis, GC-2 cell, hypoxia, lycopene, miR-23a, miR-23b, PROK2, varicocele.

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