Elevated blood urea nitrogen alters the transcriptome of equine embryos
Yatta Linhares Boakari
A B , Hossam El-Sheikh Ali A C , Pouya Dini A D , Shavahn Loux A , Claudia Barbosa Fernandes A E , Kirsten Scoggin A , Alejandro Esteller-Vico A F , Laurie Lawrence G and Barry Ball A H
+ Author Affiliations
- Author Affiliations
A Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, 1400 Nicholasville Road, Lexington, KY 40546, USA.
B Department of Clinical Sciences, Auburn University College of Veterinary Medicine, 1010 Wire Road, Auburn, AL 36849, USA.
C Theriogenology Department, University of Mansoura, 25 El Gomhouria Street, Mansoura, 35516, Egypt.
D Faculty of Veterinary Medicine, Ghent University, Sint-Pietersnieuwstraat 33, Merelbeke, B-9820, Belgium.
E Department of Animal Reproduction, Rua da Reitoria, 374, University of São Paulo, São Paulo, 05508-270, Brazil.
F Department of Biomedical and Diagnostic Sciences, University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA.
G Department of Animal Science, University of Kentucky, 1400 Nicholasville Road, Lexington, KY 40546, USA.
H Corresponding author. Email: b.a.ball@uky.edu
Reproduction, Fertility and Development 32(16) 1239-1249 https://doi.org/10.1071/RD20088
Submitted: 1 April 2020 Accepted: 17 August 2020 Published: 28 October 2020
Abstract
High blood urea nitrogen (BUN) in cows and ewes has a negative effect on embryo development; however, no comparable studies have been published in mares. The aims of the present study were to evaluate the effects of high BUN on blastocoele fluid, systemic progesterone and Day 14 equine embryos. When a follicle with a mean (±s.e.m.) diameter of 25 ± 3 mm was detected, mares were administered urea (0.4 g kg−1) with sweet feed and molasses (n = 9) or sweet feed and molasses alone (control; n = 10). Blood samples were collected every other day. Mares were subjected to AI and the day ovulation was detected was designated as Day 0. Embryos were collected on Day 14 (urea-treated, n = 5 embryos; control, n = 7 embryos). There was an increase in systemic BUN in the urea-treated group compared with control (P < 0.05), with no difference in progesterone concentrations. There were no differences between the two groups in embryo recovery or embryo size. Urea concentrations in the blastocoele fluid tended to be higher in the urea-treated mares, with a strong correlation with plasma BUN. However, there was no difference in the osmolality or pH of the blastocoele fluid between the two groups. Differentially expressed genes in Day 14 embryos from urea-treated mares analysed by RNA sequencing were involved in neurological development, urea transport, vascular remodelling and adhesion. In conclusion, oral urea treatment in mares increased BUN and induced transcriptome changes in Day 14 equine embryos of genes important in normal embryo development.
Keywords: blastocoele fluid, embryo development, high-protein diet, horse.
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