Involvement of peroxiredoxin 2 in cumulus expansion and oocyte maturation in mice
You-Jee Jang A , Jin-Seon Kim B , Pu-Reum Yun B , Young-Woo Seo A , Tae-Hoon Lee C , Jae-Il Park A D and Sang-Young Chun
B D
+ Author Affiliations
- Author Affiliations
A Animal Facility of Aging Science, Korea Basic Science Institute, Gwangju 61186, Republic of Korea.
B School of Biological Sciences and Biotechnology, Faculty of Life Science, Chonnam National University, Gwangju 61186, Republic of Korea.
C Department of Oral Biochemistry, College of Dentistry, Chonnam National University, Gwangju 61186, Republic of Korea.
D Corresponding authors. Email: jaeil74@kbsi.re.kr; sychun@jnu.ac.kr
Reproduction, Fertility and Development 32(8) 783-791 https://doi.org/10.1071/RD19310
Submitted: 3 April 2019 Accepted: 30 November 2019 Published: 7 May 2020
Journal Compilation © CSIRO 2020 Open Access CC BY-NC-ND
Abstract
Peroxiredoxin 2 (Prdx2), an antioxidant enzyme, is expressed in the ovary during the ovulatory process. The aim of the present study was to examine the physiological role of Prdx2 during ovulation using Prdx2-knockout mice and mouse cumulus–oocyte complex (COC) from WT mice. Two days of treatment of immature mice (21–23 days old) with equine chorionic gonadotrophin and followed by treatment with human chorionic gonadotrophin greatly impaired cumulus expansion and oocyte maturation in Prdx2-knockout but not wild-type mice. Treatment of COCs in culture with conoidin A (50 µM), a 2-cys Prdx inhibitor, abolished epiregulin (EPI)-induced cumulus expansion. Conoidin A treatment also inhibited EPI-stimulated signal molecules, including signal transducer and activator of transcription-3, AKT and mitogen-activated protein kinase 1/2. Conoidin A treatment also reduced the gene expression of EPI-stimulated expansion-inducing factors (hyaluronan synthase 2 (Has2), pentraxin 3 (Ptx3), TNF-α induced protein 6 (Tnfaip6) and prostaglandin-endoperoxide synthase 2 (Ptgs2)) and oocyte-derived factors (growth differentiation factor 9 (Gdf9) and bone morphogenetic protein 15 (Bmp15)). Furthermore, conoidin A inhibited EPI-induced oocyte maturation and the activity of connexins 43 and 37. Together, these results demonstrate that Prdx2 plays a role in regulating cumulus expansion and oocyte maturation during the ovulatory process in mice, probably by modulating epidermal growth factor receptor signalling.
Additional keywords: cumulus cell, Graafian follicle, ovary, ovulation.
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